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prime-it rmt random primer labeling kit  (Promega)

 
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    Promega prime-it rmt random primer labeling kit
    Prime It Rmt Random Primer Labeling Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+primer+labelling+method/prime+it+rmt+random+primer+labeling+kit/pm09027499-57-22-28
    Average 90 stars, based on 1 article reviews
    prime-it rmt random primer labeling kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Genomic organization and expression of the human fatty aldehyde dehydrogenase gene (FALDH).
    Article Snippet: .. The probes were radioactively labeled with [a-32P]dCTP and sequenced from both ends with the fmol Cycle Sequencing kit (3000 Ci/mmol) using the Prime-It RmT Random Primer Labeling kit (Promega Corp.) to determine the location of the exon/intron junction. and hybridized with the blotted YAC DNA in ExpressHyb solution. ..

    Sequencing:

    Article Title: Genomic organization and expression of the human fatty aldehyde dehydrogenase gene (FALDH).
    Article Snippet: .. The probes were radioactively labeled with [a-32P]dCTP and sequenced from both ends with the fmol Cycle Sequencing kit (3000 Ci/mmol) using the Prime-It RmT Random Primer Labeling kit (Promega Corp.) to determine the location of the exon/intron junction. and hybridized with the blotted YAC DNA in ExpressHyb solution. ..



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    Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

    Journal: Journal of Diabetes

    Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

    doi: 10.1111/1753-0407.13471

    Figure Lengend Snippet: Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

    Article Snippet: These circRNAs were amplified and transcribed into fluorescent cRNA using a random primer method (Arraystar Super RNA Labeling Kit; Arraystar). labeled cRNAs that were hybridized to Arraystar Rat circRNA Array V2.0 (8 × 15 K) with a total of 14 145 circRNA probes on the chip.

    Techniques: Quantitative Proteomics

    Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

    Journal: Journal of Diabetes

    Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

    doi: 10.1111/1753-0407.13471

    Figure Lengend Snippet: Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

    Article Snippet: These circRNAs were amplified and transcribed into fluorescent cRNA using a random primer method (Arraystar Super RNA Labeling Kit; Arraystar). labeled cRNAs that were hybridized to Arraystar Rat circRNA Array V2.0 (8 × 15 K) with a total of 14 145 circRNA probes on the chip.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot, Flow Cytometry