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random hexanucleotide primers prime-a-gene  (Promega)

 
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    Structured Review

    Promega random hexanucleotide primers prime-a-gene
    Random Hexanucleotide Primers Prime A Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+hexanucleotide+primers/random+hexanucleotide+primers+prime+a+gene/pm08702559-78-12-16
    Average 90 stars, based on 1 article reviews
    random hexanucleotide primers prime-a-gene - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Fibronectin mRNA splice variant in articular cartilage lacks bases encoding the V, III-15, and I-10 protein segments.
    Article Snippet: .. Radiolabeled probes were prepared from gel-purified cDNA insert preps using [32P]dCTP and random hexanucleotide primers (Prime-a-Gene, Promega) and purified with Sephadex G-50 spin columns (Boehringer Mannheim). ..

    Article Title: Expression of p21CIP1/WAF1 in chondrocytes.
    Article Snippet: During endochondral ossification, proliferative activity of chondrocytes is arrested and the cells undergo terminal hypertrophic differentiation.. We examined the expression of the cyclin-dependent kinase inhibitor, p21 in permanent cartilage (xyphoid and articular cartilage) and in cartilage undergoing endochondral ossification (growth plate, epiphyseal ossification centers, and costochondral junctions) to determine if p21 is up-regulated in chondrocytes during hypertrophic differentiation.. Northern blot analyses demonstrated expression of p21 in chondrocytes undergoing endochondral ossification and from sites of permanent cartilage.

    Positive Control:

    Article Title: Expression of p21CIP1/WAF1 in chondrocytes.
    Article Snippet: During endochondral ossification, proliferative activity of chondrocytes is arrested and the cells undergo terminal hypertrophic differentiation.. We examined the expression of the cyclin-dependent kinase inhibitor, p21 in permanent cartilage (xyphoid and articular cartilage) and in cartilage undergoing endochondral ossification (growth plate, epiphyseal ossification centers, and costochondral junctions) to determine if p21 is up-regulated in chondrocytes during hypertrophic differentiation.. Northern blot analyses demonstrated expression of p21 in chondrocytes undergoing endochondral ossification and from sites of permanent cartilage.

    Negative Control:

    Article Title: Expression of p21CIP1/WAF1 in chondrocytes.
    Article Snippet: During endochondral ossification, proliferative activity of chondrocytes is arrested and the cells undergo terminal hypertrophic differentiation.. We examined the expression of the cyclin-dependent kinase inhibitor, p21 in permanent cartilage (xyphoid and articular cartilage) and in cartilage undergoing endochondral ossification (growth plate, epiphyseal ossification centers, and costochondral junctions) to determine if p21 is up-regulated in chondrocytes during hypertrophic differentiation.. Northern blot analyses demonstrated expression of p21 in chondrocytes undergoing endochondral ossification and from sites of permanent cartilage.

    Activity Assay:

    Article Title: Transplanted Oligodendrocyte Progenitor Cells Expressing a Dominant-Negative FGF Receptor Transgene Fail to Migrate In Vivo
    Article Snippet: Total RNA (10 μg) was separated on a 1.5% agarose gel containing 2.2 m formaldehyde, transferred overnight to a nylon membrane (Oncor, Gaithersburg, MD), and cross-linked under ultraviolet light using a Stratalinker (Stratagene, La Jolla, CA). .. Radiolabeled FGFR1 cDNA probes were prepared using random hexanucleotide primers (Prime-a-Gene; Promega, Madison, WI) with [α- 32 P]dCTP (specific activity, 3000 Ci/mmole; Amersham). .. The blot was prehybridized for 2 hr at 42°C in Hybrisol I (Oncor), hybridized with labeled probe for 14 hr at 42°C, washed at high stringency (0.2× SSC, 0.2% SDS; 67°C), and then exposed to Fuji RX film for autoradiography.

    Article Title: Transplanted Oligodendrocyte Progenitor Cells Expressing a Dominant-Negative FGF Receptor Transgene Fail to MigrateIn Vivo
    Article Snippet: Total RNA (10 mg) was separated on a 1.5% agarose gel containing 2.2 M formaldehyde, transferred overnight to a nylon membrane (Oncor, Gaithersburg, MD), and cross-linked under ultraviolet light using a Stratalinker (Stratagene, La Jolla, CA). .. Radiolabeled FGFR1 cDNA probes were prepared using random hexanucleotide primers (Prime-a-Gene; Promega, Madison, WI) with [a-32P]dCTP (specific activity, 3000 Ci/mmole; Amersham). .. The blot was prehybridized for 2 hr at 42°C in Hybrisol I (Oncor), hybridized with labeled probe for 14 hr at 42°C, washed at high stringency (0.23 SSC, 0.2% SDS; 67°C), and then exposed to Fuji RX film for autoradiography.



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