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Structured Review

Affinity Biosciences rabbit polyclonal anti tnf α antibody
Immunohistochemical localization <t>of</t> <t>TNF-α</t> in skin flaps of experimental groups. Representative sections <t>showing</t> <t>TNF-α</t> expression in the control (A), low-dose EMF (B), and high-dose EMF (C) groups. Black arrows indicate TNF-α immunoreactivity in keratinocytes, fibroblasts, and inflammatory cells. Stronger cytoplasmic immunoreactivity was observed in the control and high-dose EMF groups compared with the low-dose EMF group. Scale bar: 50 μm; magnification × 40.
Rabbit Polyclonal Anti Tnf α Antibody, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal/anti+tnf+%CE%B1/pmc13280331-76-8-12
Average 86 stars, based on 1 article reviews
rabbit polyclonal anti tnf α antibody - by Bioz Stars, 2026-11
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Images

1) Product Images from "Effects of extremely low-frequency sinusoidal electromagnetic field therapy on survival and vascularization in a rat random-pattern skin flap model"

Article Title: Effects of extremely low-frequency sinusoidal electromagnetic field therapy on survival and vascularization in a rat random-pattern skin flap model

Journal: JPRAS Open

doi: 10.1016/j.jpra.2026.05.040

Immunohistochemical localization of TNF-α in skin flaps of experimental groups. Representative sections showing TNF-α expression in the control (A), low-dose EMF (B), and high-dose EMF (C) groups. Black arrows indicate TNF-α immunoreactivity in keratinocytes, fibroblasts, and inflammatory cells. Stronger cytoplasmic immunoreactivity was observed in the control and high-dose EMF groups compared with the low-dose EMF group. Scale bar: 50 μm; magnification × 40.
Figure Legend Snippet: Immunohistochemical localization of TNF-α in skin flaps of experimental groups. Representative sections showing TNF-α expression in the control (A), low-dose EMF (B), and high-dose EMF (C) groups. Black arrows indicate TNF-α immunoreactivity in keratinocytes, fibroblasts, and inflammatory cells. Stronger cytoplasmic immunoreactivity was observed in the control and high-dose EMF groups compared with the low-dose EMF group. Scale bar: 50 μm; magnification × 40.

Techniques Used: Immunohistochemical staining, Expressing, Control

Related Articles

other:

Article Title: Self-traceable, Self-protective, and self-sufficient combinational catalytic therapy of inflammatory bowel disease by a smart Nanozyme-DNAzyme platform
Article Snippet: DNAzyme-based gene silencing therapies are promising candidates for the treatment of inflammatory bowel disease (IBD).. However, DNAzyme’s unique substrate, structural instability, and conditional catalytic mechanism necessitate delivery systems enabling concurrent integration of precision (gene silencing surveillance), durability (degradation resistance), and efficacy (sustained catalytic output).. Herein, we propose a smart photoacoustic imaging (PAI)-guided nanozyme-DNAzyme platform for combinational catalytic therapy (NDCT) of IBD by introducing the antioxidant iridium nanozyme (IrNZ) as a protector for DNAzyme and in-situ amplifier of PAI signal.

Article Title: Hydrogen gas (H 2 ) pretreatment improves lipopolysaccharide-induced acute liver injury in mice by inhibiting NLRP3 inflammasome activation and pyroptosis signaling
Article Snippet: Tumor necrosis factor α (TNF-α) antibody , #AF7014 , 1:1000 , Affinity Biosciences (Changzhou, Jiangsu, China).

Incubation:

Article Title: Effects of extremely low-frequency sinusoidal electromagnetic field therapy on survival and vascularization in a rat random-pattern skin flap model
Article Snippet: .. Sections were incubated overnight at 4 °C with rabbit polyclonal anti-TNF-α antibody (Affinity Biosciences, catalog no AF7014; dilution 1:100). ..

Article Title: Effects of very early arthritis on pain, behavior, and hippocampal TNF/BDNF in rats.
Article Snippet: .. After blocking endogenous peroxidase activity, sections were incubated overnight at 4 °C with primary antibodies: anti-TNF-α (AF7014; Affinity Biosciences, Cincinnati, OH) and anti-BDNF (25699-1-AP; Proteintech, Rosemont, IL). ..

Article Title: Effects of Extremely Low-Frequency Sinusoidal Electromagnetic Field Therapy on Survival and Vascularization in a Rat Random-Pattern Skin Flap Model
Article Snippet: .. Sections were incubated overnight at 4°C with rabbit polyclonal anti-TNF-α antibody (Affinity Biosciences, catalog no. AF7014; dilution 1:100). ..

Blocking Assay:

Article Title: Effects of very early arthritis on pain, behavior, and hippocampal TNF/BDNF in rats.
Article Snippet: .. After blocking endogenous peroxidase activity, sections were incubated overnight at 4 °C with primary antibodies: anti-TNF-α (AF7014; Affinity Biosciences, Cincinnati, OH) and anti-BDNF (25699-1-AP; Proteintech, Rosemont, IL). ..

Activity Assay:

Article Title: Effects of very early arthritis on pain, behavior, and hippocampal TNF/BDNF in rats.
Article Snippet: .. After blocking endogenous peroxidase activity, sections were incubated overnight at 4 °C with primary antibodies: anti-TNF-α (AF7014; Affinity Biosciences, Cincinnati, OH) and anti-BDNF (25699-1-AP; Proteintech, Rosemont, IL). ..



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( A–C ) Fura-2 calcium imaging assay measuring SOCE in cells treated with Scr shRNA or different shRNA sequences against SNAP23 ( A ) SNAP25 ( B ) SNAP29 ( C ). Average traces from 30 to 40 cells per group are shown. ( D ) Western blot images of co-IPs to test the association of Orai1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with Orai1-Myc and untagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2) ( E ) Western blot images of co-IP to test the association of Stim1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with YFP-Stim1 and Myc-tagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2). Figure 2—figure supplement 3—source data 1. Numerical data underlying the quantitative analyses represented in  .  Figure 2—figure supplement 3—source data 2. Folder containing labeled PDF files of the original western blots for  .  Figure 2—figure supplement 3—source data 3. Folder containing the original images of western blots shown in  .
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Image Search Results


( A–C ) Fura-2 calcium imaging assay measuring SOCE in cells treated with Scr shRNA or different shRNA sequences against SNAP23 ( A ) SNAP25 ( B ) SNAP29 ( C ). Average traces from 30 to 40 cells per group are shown. ( D ) Western blot images of co-IPs to test the association of Orai1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with Orai1-Myc and untagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2) ( E ) Western blot images of co-IP to test the association of Stim1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with YFP-Stim1 and Myc-tagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2). Figure 2—figure supplement 3—source data 1. Numerical data underlying the quantitative analyses represented in  .  Figure 2—figure supplement 3—source data 2. Folder containing labeled PDF files of the original western blots for  .  Figure 2—figure supplement 3—source data 3. Folder containing the original images of western blots shown in  .

Journal: eLife

Article Title: Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking

doi: 10.7554/eLife.111335

Figure Lengend Snippet: ( A–C ) Fura-2 calcium imaging assay measuring SOCE in cells treated with Scr shRNA or different shRNA sequences against SNAP23 ( A ) SNAP25 ( B ) SNAP29 ( C ). Average traces from 30 to 40 cells per group are shown. ( D ) Western blot images of co-IPs to test the association of Orai1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with Orai1-Myc and untagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2) ( E ) Western blot images of co-IP to test the association of Stim1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with YFP-Stim1 and Myc-tagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2). Figure 2—figure supplement 3—source data 1. Numerical data underlying the quantitative analyses represented in . Figure 2—figure supplement 3—source data 2. Folder containing labeled PDF files of the original western blots for . Figure 2—figure supplement 3—source data 3. Folder containing the original images of western blots shown in .

Article Snippet: Antibody , Rabbit polyclonal anti-SNAP23 , SYSY , 111203 , WB (1:1500).

Techniques: Imaging, shRNA, Western Blot, Transfection, Co-Immunoprecipitation Assay, Labeling

Journal: eLife

Article Title: Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking

doi: 10.7554/eLife.111335

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-SNAP23 , SYSY , 111203 , WB (1:1500).

Techniques: