Review



rabbit anti map2  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc rabbit anti map2
    Rabbit Anti Map2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/pmc13058276-4-0-3
    Average 86 stars, based on 1 article reviews
    rabbit anti map2 - by Bioz Stars, 2026-11
    86/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Mechanisms of inhibitory transmission at hippocampal synapses
    Article Snippet: Neurons were harvested at DIV17-18 by lysis and reverse transcribed to synthesize cDNA using a Power SYBR Green Cells-to-CT kit (Life Technologies). .. Antibodies used in the NLGN4X study were anti-NLGN4X (Sigma), anti-NLGN4X (abcam), anti-pan NLGN 4F9 (Synaptic Systems), anti-GST (Bethyl Laboratories), antiFLAG (Sigma), anti-HA rat (Roche), anti-HA rabbit (Abcam), anti GluA1 pS831 (Covance), anti-PSD-95 (Neuromab), anti-VGLUT1 (Millipore), anti-MAP2 (Cell Signaling), and anti-actin (ABM) and were used at a concentration of 1 μg/μL. ..

    Incubation:

    Article Title: Multilineage potential induced by reconstruction of three-dimensional structures in mouse fetal brain cells.
    Article Snippet: We examined whether the multilineage potential of mouse fetal brain cells is induced by the three-dimensional (3D) structural reconstruction.. Dissociated single cells from the cerebral hemispheres of mouse fetuses at embryonic day 14.5–15 (E14.5–15) rapidly aggregated into cell clusters, forming spherical cell aggregates (SCAs).. Immunofluorescence staining revealed that SCAs were positive for Map2 but negative for Nestin, GFAP, and markers of undifferentiated cells such as Oct4, SSEA-1, ALDH2, and CD133.

    Article Title: A Blood-Brain Barrier-Penetrant Ag(III) Corrole Compound Rescues Alzheimer's Disease Pathology by Targeting Aβ42-Induced Oxidative Stress.
    Article Snippet: .. They were then incubated overnight at 4 °C with rabbit anti-MAP2 (1:150, Cell Signaling Technology) or Chicken IgG MAP2 (1:1000, Invitrogen) and rabbit IgG Aβ42 (1:200, Invitrogen) antibody diluted in 5% BSA. .. After washing, Alexa 488-conjugated anti-rabbit IgG (1:1000, Cell Signaling Technology) was used as the secondary antibody.

    Article Title: A Blood‐Brain Barrier‐Penetrant Ag(III) Corrole Compound Rescues Alzheimer's Disease Pathology by Targeting Aβ42‐Induced Oxidative Stress
    Article Snippet: .. They were then incubated overnight at 4 °C with rabbit anti‐MAP2 (1:150, Cell Signaling Technology) or Chicken IgG MAP2 (1:1000, Invitrogen) and rabbit IgG Aβ42 (1:200, Invitrogen) antibody diluted in 5% BSA. .. After washing, Alexa 488‐conjugated anti‐rabbit IgG (1:1000, Cell Signaling Technology) was used as the secondary antibody.

    Marker:

    Article Title: Multilineage potential induced by reconstruction of three-dimensional structures in mouse fetal brain cells.
    Article Snippet: We examined whether the multilineage potential of mouse fetal brain cells is induced by the three-dimensional (3D) structural reconstruction.. Dissociated single cells from the cerebral hemispheres of mouse fetuses at embryonic day 14.5–15 (E14.5–15) rapidly aggregated into cell clusters, forming spherical cell aggregates (SCAs).. Immunofluorescence staining revealed that SCAs were positive for Map2 but negative for Nestin, GFAP, and markers of undifferentiated cells such as Oct4, SSEA-1, ALDH2, and CD133.

    other:

    Article Title: Ginsenoside Rb1 Prevents MPTP-Induced Changes in Hippocampal Memory via Regulation of the α-Synuclein/PSD-95 Pathway
    Article Snippet: Memory deficiency is a common non-motor symptom of Parkinson's disease (PD), and conventionally, α-synuclein is considered to be an important biomarker for both motor and cognitive characteristics attributed to PD.. However, the role of physiological α-synuclein in cognitive impairment remains undetermined.. Ginsenoside Rb1 has been shown to protect dopaminergic neurons (DA) from death and inhibit α-synuclein fibrillation and toxicity in vitro.

    Cell-Signaling:

    Article Title: A Blood‐Brain Barrier‐Penetrant Ag(III) Corrole Compound Rescues Alzheimer's Disease Pathology by Targeting Aβ42‐Induced Oxidative Stress
    Article Snippet: .. They were then incubated overnight at 4 °C with rabbit anti‐MAP2 (1:150, Cell Signaling Technology) or Chicken IgG MAP2 (1:1000, Invitrogen) and rabbit IgG Aβ42 (1:200, Invitrogen) antibody diluted in 5% BSA. .. After washing, Alexa 488‐conjugated anti‐rabbit IgG (1:1000, Cell Signaling Technology) was used as the secondary antibody.

    Article Title: DCPS modulates TDP-43-linked neurodegeneration through P-body-mediated RNA decay
    Article Snippet: Rabbit anti-FLAG , Proteintech , Cat #20543–1-AP; RRID: AB_11232216. .. Rabbit anti-MAP2 , Cell Signaling Technology , Cat #8707; RRID: AB_2722660. .. Chicken anti-MAP2 , Biosensis , Cat #C-1382–50; RRID: AB_2492335.



    Similar Products

    rabbit anti map2  (Proteintech)
    96
    Proteintech rabbit anti map2
    Rabbit Anti Map2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+Antibody/pm42849603-53-26-29
    Average 96 stars, based on 1 article reviews
    rabbit anti map2 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    map2  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology map2
    Map2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+Rabbit+pAb/pm42822280-58-9-52
    Average 94 stars, based on 1 article reviews
    map2 - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    map2 anti rabbit  (Synaptic Systems)
    94
    Synaptic Systems map2 anti rabbit
    Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker <t>MAP2,</t> and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.
    Map2 Anti Rabbit, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/188+003/pmc13415681-9-0-4
    Average 94 stars, based on 1 article reviews
    map2 anti rabbit - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    fitc conjugated rabbit anti mouse map2 primary antibody  (Wuhan Fine Biotech)
    86
    Wuhan Fine Biotech fitc conjugated rabbit anti mouse map2 primary antibody
    Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker <t>MAP2,</t> and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.
    Fitc Conjugated Rabbit Anti Mouse Map2 Primary Antibody, supplied by Wuhan Fine Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/anti+conjugated+goat+hrp+igg+rabbit/pm42056801-140-1-7
    Average 86 stars, based on 1 article reviews
    fitc conjugated rabbit anti mouse map2 primary antibody - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    protein 2  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology protein 2
    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of <t>MAP2,</t> PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.
    Protein 2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+Rabbit+pAb/pmc13153083-46-4-24
    Average 94 stars, based on 1 article reviews
    protein 2 - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    rabbit anti map2  (Novus Biologicals)
    93
    Novus Biologicals rabbit anti map2
    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of <t>MAP2,</t> PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.
    Rabbit Anti Map2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+Antibody/pmc13104655-145-64-68
    Average 93 stars, based on 1 article reviews
    rabbit anti map2 - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    rabbit anti map2 polyclonal  (Proteintech)
    96
    Proteintech rabbit anti map2 polyclonal
    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of <t>MAP2,</t> PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.
    Rabbit Anti Map2 Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+Antibody/pmc13045046-1-0-4
    Average 96 stars, based on 1 article reviews
    rabbit anti map2 polyclonal - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    cell signalling technology 8707  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc cell signalling technology 8707
    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of <t>MAP2,</t> PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.
    Cell Signalling Technology 8707, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+XP+Rabbit+mAb/pm41915835-200-53-53
    Average 96 stars, based on 1 article reviews
    cell signalling technology 8707 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    resource source identifier antibodies map2 d5g1 xp rabbit mab cell signalling technology  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc resource source identifier antibodies map2 d5g1 xp rabbit mab cell signalling technology
    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of <t>MAP2,</t> PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.
    Resource Source Identifier Antibodies Map2 D5g1 Xp Rabbit Mab Cell Signalling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/pm41915835-215-2-11
    Average 86 stars, based on 1 article reviews
    resource source identifier antibodies map2 d5g1 xp rabbit mab cell signalling technology - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    anti map2  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti map2
    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of <t>MAP2,</t> PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.
    Anti Map2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+map2/MAP2+XP+Rabbit+mAb/bio_rxiv__64898__2026__03__28__714989-72-26-28
    Average 96 stars, based on 1 article reviews
    anti map2 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker MAP2, and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.

    Journal: Cell Reports

    Article Title: Striatal neuron dysfunction in C9ORF72-FTD/ALS is driven by AIS and potassium channel dysregulation

    doi: 10.1016/j.celrep.2026.117672

    Figure Lengend Snippet: Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker MAP2, and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.

    Article Snippet: MAP2 anti-Rabbit (1:1000) , Synaptic Systems , Cat# 188 003; RRID AB_2281442.

    Techniques: Staining, Marker

    OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of MAP2, PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: OMT reversed the neurons damage and mitophagy reduction in the hippocampal of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of MAP2, PSD95 and SYN, n = 5; (C) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (D) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (E,F) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Immunofluorescence, Expressing, Electron Microscopy, Control

    OMT restored the mitophagy reduction and the SIRT1 inhibition in the hippocampus of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of PINK1and PARKIN, n = 5; (C) The mRNA expression of PINK1and PARKIN, n = 6; (D) Binding energy of OMT with downstream targets and binding sites of OMT to SIRT1; (E,F) Representative immunofluorescence images and quantitative analysis (n = 5), mRNA expression (n = 6) of SIRT1; (G,H) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: OMT restored the mitophagy reduction and the SIRT1 inhibition in the hippocampus of CCH mice. (A,B) Representative immunofluorescence images and quantitative analysis of PINK1and PARKIN, n = 5; (C) The mRNA expression of PINK1and PARKIN, n = 6; (D) Binding energy of OMT with downstream targets and binding sites of OMT to SIRT1; (E,F) Representative immunofluorescence images and quantitative analysis (n = 5), mRNA expression (n = 6) of SIRT1; (G,H) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Inhibition, Immunofluorescence, Expressing, Binding Assay, Control

    OMT reversed the OGD/R induced HT22 cell dysfunction, mitophagy inhibition and decreased SIRT1 expression. (A) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (B) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (C) The mRNA expression of PINK1, PARKIN and SIRT1, n = 6; (D) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3; (E) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (F) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (G) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: OMT reversed the OGD/R induced HT22 cell dysfunction, mitophagy inhibition and decreased SIRT1 expression. (A) The mRNA expression of MAP2, PSD95 and SYN, n = 6; (B) The protein expression and quantitative analysis of MAP2, PSD95 and SYN, n = 3; (C) The mRNA expression of PINK1, PARKIN and SIRT1, n = 6; (D) The protein expression and quantitative analysis of PINK1, PARKIN and SIRT1, n = 3; (E) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (F) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (G) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Inhibition, Expressing, Fluorescence, Microscopy, Control

    SIRT1 and mitophagy mediate the protective effect of OMT on OGD/R HT22 cells. (A) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (B,C) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3; (D) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (E) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (F) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: SIRT1 and mitophagy mediate the protective effect of OMT on OGD/R HT22 cells. (A) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (B,C) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3; (D) Detection of JC-1 aggregates (red) and monomers (green) by confocal fluorescence microscopy, 1000×, n = 3; (E) Colocalization of lysosome (green) and mitochondria (red) by confocal fluorescence microscopy, 400×, 1000×, n = 3; (F) Representative ROS images, 100×, n = 5. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Expressing, Fluorescence, Microscopy, Control

    SIRT1 mediates the neuroprotective effect and mitophagy of OMT on the hippocampus of CCH mice. (A,B) The representative traces and quantitative analysis in OFT, Y-maze and NOR test, n = 6; (C,D) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6; (E,F) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (G,H) Representative immunofluorescence images and quantitative analysis of MAP2 and PINK1, n = 5; (I,J) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Journal: Frontiers in Pharmacology

    Article Title: Oxymatrine alleviates cognitive dysfunction in mice caused by chronic cerebral hypoperfusion by promoting SIRT1/ PINK1-mediated mitophagy

    doi: 10.3389/fphar.2026.1736022

    Figure Lengend Snippet: SIRT1 mediates the neuroprotective effect and mitophagy of OMT on the hippocampus of CCH mice. (A,B) The representative traces and quantitative analysis in OFT, Y-maze and NOR test, n = 6; (C,D) The mass of mitochondria and the number of mitophagosomes observed by electron microscopy, n = 6; (E,F) The mRNA expression of MAP2, PSD95, SYN PINK1 and PARKIN, n = 6; (G,H) Representative immunofluorescence images and quantitative analysis of MAP2 and PINK1, n = 5; (I,J) The protein expression and quantitative analysis of MAP2, PSD95, SYN PINK1 and PARKIN, n = 3. Means ± S.E.M. * P < 0.05, ** P < 0.01 vs. the corresponding control. CCH, chronic cerebral hypoperfusion; OMT, oxymatrine; MAP2, microtubule-associated protein 2; PSD95, postsynaptic density protein-95; SYN, synaptophysin; PINK1, PTEN-induced kinase 1; PARKIN, parkinson disease protein 2; SIRT1, Sirtuin 1.

    Article Snippet: The antibodies of microtubule-associated protein 2 (MAP2, A2572), postsynaptic density protein-95 (PSD95, A6194), synaptophysin (SYN, A6344) and sirtuin 1 (SIRT1, A11267) were sourced from Abclonal Technology Co., Ltd., Wuhan, China.

    Techniques: Electron Microscopy, Expressing, Immunofluorescence, Control