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AH Diagnostics quickchange method
Quickchange Method, supplied by AH Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickchange/quickchange+method/pm15670165-105-12-15
Average 90 stars, based on 1 article reviews
quickchange method - by Bioz Stars, 2026-10
90/100 stars

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Mutagenesis:

Article Title: Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity.
Article Snippet: .. Site-directed mutagenesis of the L. lactis pyrG gene was performed using the QuickChange method (Stratagene, AH Diagnostics, Aarhus, Denmark) and the complementary deoxy-oligonucleotides LL5-R359M; GGCTTTG GTCAAATGGGAACAGAAGGT, LL3-R359M; ACCTTC TGTTCCCATTTGACCAAAGCC, LL5-R359P; GGCTTT GGTCAACCGGGAACAGAAGGT, LL3-R359P; ACCTT CTGTTCCCGGTTGACCAAAGCC, LL5-G360A; TTTGG TCAACGTGCAACAGAAGGTAAG, LL3-G360A; CTTA CCTTCTGTTGCACGTTGACCAAA, LL5-G360P; TTTGG TCAACGTCCAACAGAAGGTAAG, LL3-G360P; CTTAC CTTCTGTTGGACGTTGACCAAA, LL5-E362Q; CAACG TGGAACACAAGGTAAGATTGCA and LL3-E362Q; TGCAATCTTACCTTGTGTTCCACGTTG for construction of the alleles encoding the R359M, R359P, G360A, G360P and E362Q enzymes, respectively. ..



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