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quickchange site directed mutagenesis kit  (Qiagen)


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    Structured Review

    Qiagen quickchange site directed mutagenesis kit
    Quickchange Site Directed Mutagenesis Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickchange+kit/quikchange+site+directed+mutagenesis+kit/pmc12273560-151-11-16
    Average 90 stars, based on 1 article reviews
    quickchange site directed mutagenesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: The Leucine-Rich Repeat Kinase 2 Variant LRRK2 G2019S Up-Regulates L-Type (CaV1.3) Calcium Channel via the Ca V β 3 Subunit: Possible Role in the Pathogenesis of Parkinson’s Disease
    Article Snippet: Mutagenic oligonucleotides were designed to introduce point mutations into the sequence of both subunits of the Ca V 1.3 channels (Ca V α 1 and Ca V β 3 ) using the commercial QuikChange XL Site-Directed Mutagenesis (QIAGEN, Hilden, Germany) kit following the manufacturer’s instructions.

    Article Title: Structural basis of transcriptional regulation by UrtR in response to uric acid.
    Article Snippet: The full-length pecS gene was mutated via the QuikChange site-directed mutagenesis protocol (Qiagen) to generate alanine mutant, glycine mutant, and deletion mutant proteins.

    Article Title: Supporting Information for Tryptophan Extends the Life of Cytochrome P450
    Article Snippet: Site-directed mutagenesis was carried out utilizing the QuikChange Site-Directed Mutagenesis kit from Qiagen, with primers designed to introduce the desired mutations, sourced from Invitrogen.

    Article Title: The Leucine-Rich Repeat Kinase 2 Variant LRRK2 G2019S Up-Regulates L-Type (CaV1.3) Calcium Channel via the Ca V β 3 Subunit: Possible Role in the Pathogenesis of Parkinson's Disease.
    Article Snippet: Mutagenic oligonucleotides were designed to introduce point mutations into the sequence of both subunits of the CaV1.3 channels (CaVα1 and CaVβ3) using the commercial QuikChange XL Site-Directed Mutagenesis (QIAGEN, Hilden, Germany) kit following the manufacturer’s instructions.

    Mutagenesis:

    Article Title: A versatile fluorescence polarization-based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim)
    Article Snippet: The USP2 catalytic domain expression construct as used for the structure determination of a covalent Ubiquitin-USP2 complex (PDB code: 2IBI ) was a gift from Cheryl Arrowsmith (Addgene plasmid # 36894; http://n2t.net/addgene:36894 ; RRID: Addgene 36,894). .. The active site cysteine USP2 C276A mutation was generated by the QuickChange site directed mutagenesis kit (Qiagen). .. The JOSD2 (residues 12–186) gene was obtained by gene synthesis from GenScript and subcloned into pET21d using forward primer AATTTTGTTTAACTTTAAGAAGGAGATATACCATGACCGTGTACCACGAACGGC and reverse primer CTCAGTGGTGGTGGTGGTGGTGGTCTGTCCGCAGCCAGCTG.

    Article Title: PUFA stabilizes a conductive state of the selectivity filter in IKs channels
    Article Snippet: .. Site-directed mutagenesis was performed using the QuikChange II XL Mutagenesis Kit (QIAGEN Sciences) for mutations in KCNQ1. .. Primer sequences for mutagenesis are (mutated residues are shown in bold and altered bases are in capital letters): 50 ng of complementary RNA was injected into defolliculated Xenopus laevis oocytes (Ecocyte Bioscience, Austin; Xenopus 1 Corp, Dexter) for channel expression.

    Article Title: A versatile fluorescence polarization based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim).
    Article Snippet: The USP2 catalytic domain expression construct as used for the structure determination of a covalent Ubiquitin-USP2 complex (PDB code: 2IBI) was a gift from Cheryl Arrowsmith (Addgene plasmid # 36894; http://n2t.net/addgene:36894; RRID: Addgene 36894). .. The active site cysteine USP2C276A mutation was generated by the QuickChange site directed mutagenesis kit (Qiagen). .. The JOSD2 (residues 12-186) gene was obtained by gene synthesis from GenScript and sub-cloned into pET21d using forward primer AATTTTGTTTAACTTTAAGAAGGAGATATACCATGACCGTGTACCACGAACGGC and reverse primer CTCAGTGGTGGTGGTGGTGGTGGTCTGTCCGCAGCCAGCTG.

    Article Title: Mutations of single residues in the complexin N-terminus exhibit distinct phenotypes in synaptic vesicle fusion
    Article Snippet: The release of neurotransmitters (NTs) at central synapses is dependent on a cascade of protein interactions, specific to the presynaptic compartment.. Among those dedicated molecules, the cytosolic complexins play an incompletely defined role as synaptic transmission regulators.. Complexins are multidomain proteins that bind soluble N-ethylmaleimide sensitive factor attachment protein receptor complexes, conferring both inhibitory and stimulatory functions.

    Generated:

    Article Title: A versatile fluorescence polarization-based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim)
    Article Snippet: The USP2 catalytic domain expression construct as used for the structure determination of a covalent Ubiquitin-USP2 complex (PDB code: 2IBI ) was a gift from Cheryl Arrowsmith (Addgene plasmid # 36894; http://n2t.net/addgene:36894 ; RRID: Addgene 36,894). .. The active site cysteine USP2 C276A mutation was generated by the QuickChange site directed mutagenesis kit (Qiagen). .. The JOSD2 (residues 12–186) gene was obtained by gene synthesis from GenScript and subcloned into pET21d using forward primer AATTTTGTTTAACTTTAAGAAGGAGATATACCATGACCGTGTACCACGAACGGC and reverse primer CTCAGTGGTGGTGGTGGTGGTGGTCTGTCCGCAGCCAGCTG.

    Article Title: A versatile fluorescence polarization based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim).
    Article Snippet: The USP2 catalytic domain expression construct as used for the structure determination of a covalent Ubiquitin-USP2 complex (PDB code: 2IBI) was a gift from Cheryl Arrowsmith (Addgene plasmid # 36894; http://n2t.net/addgene:36894; RRID: Addgene 36894). .. The active site cysteine USP2C276A mutation was generated by the QuickChange site directed mutagenesis kit (Qiagen). .. The JOSD2 (residues 12-186) gene was obtained by gene synthesis from GenScript and sub-cloned into pET21d using forward primer AATTTTGTTTAACTTTAAGAAGGAGATATACCATGACCGTGTACCACGAACGGC and reverse primer CTCAGTGGTGGTGGTGGTGGTGGTCTGTCCGCAGCCAGCTG.



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