quickblock western blot blocking buffer (Beyotime)

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Beyotime quickblock western blot blocking buffer
Quickblock Western Blot Blocking Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickblock+western+blot+blocking+buffer/quickblocktm+blocking+buffer+for+western+blot/pm39828780-98-10-15
Average 90 stars, based on 1 article reviews

quickblock western blot blocking buffer - by Bioz Stars, 2026-09

90/100 stars

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Blocking Assay:

Article Title: A Single-Chain Variable Fragment Antibody Alleviates Inflammation and Apoptosis of Neurons by Inhibiting Tau Aggregation.
Article Snippet: .. Proteins in the gel were transferred to PVDF membranes (Millipore, Bayswater, Australia, Millipore Cat# IPVH00010), which were blocked with QuickBlockTM Blocking Buffer (Beyotime Cat# P0252) followed by overnight incubation with primary antibodies (anti-βactin, 1:1000, Abcam Cat# 8226; anti-GAPDH, 1:1000, Proteintech Cat# 10494-1-AP; anti-Flag (DYKDDDDK), 1:1000, Proteintech Cat# 20543-1-AP; anti-Bax, 1:1000, AbMART, Shanghai, China, Abmart Cat# T40051F; anti-Bcl-2, 1:1000, Abmart Cat# T40056F; anti-Caspase-3, 1:1000, Abmart Cat# T40044F; anti-NF-κB p65, 1:1000, Abmart Cat# T55034F; anti-p-NF-κB p65 (Ser529), 1:500, Abmart Cat# TP56371F and anti-Tau 4R antibody, 1:1000, Abcam Cat# AB218314) diluted in QuickBlockTM Primary Antibody Dilution Buffer (Beyotime Cat# P0256). .. The membranes were subsequently washed three times with TBST for 10 min each time and then incubated with HRP-conjugated secondary antibodies (goat anti-mouse HRP-conjugated, 1:7500, BBI Cat# D110087-0100; goat anti-rabbit HRP conjugated, 1:7500, proteintech Cat# SA00001-2) for 1 h at RT and washed three times with TBST for 10 min each time.

Article Title: Deliver CEBPE via cartilage targeting Lipid nanoparticle to block CEBPE-LTF-STAT3 positive feedback loop for efficient treatment of cartilage endplate degeneration
Article Snippet: Denatured proteins were separated using SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). .. The membranes were blocked with QuickBlockTM Western blocking buffer (Beyotime, Cat. No: P0252) at room temperature for 30 min, followed by overnight incubation at 4 °C with the following primary antibodies: CEBPE (1:1000, Santa Cruz, sc-515192), Collagen II (1:1000, Proteintech, 28459-1-AP), MMP13 (1:1000, Proteintech, 18165-1-AP), RUNX2 (1:1000, Abcam, ab236639), OPN (1:1000, Abcam, ab166709), IL-1β (1:1000, Proteintech, 26048-1-AP), TNF (1:1000, Proteintech, 17590-1-AP), JAK2 (1:1000, Abcam, ab108596), P-JAK2 (1:1000, Abcam, ab32101), STAT3 (1:1000, Abcam, ab68153), P-STAT3 (1:1000, Abcam, ab267373), α-Tubulin (1:10000, Proteintech, 11224-1-AP), and GAPDH (1:50000, Proteintech, 60004-1-Ig). .. Following incubation, the membranes underwent three washes with TBST buffer, then were incubated with the respective secondary antibodies at ambient temperature for a duration of 2 h. Detection of chemiluminescence was accomplished using an advanced chemiluminescent reagent (UElandy, Product Code: S6009M), and the resultant signals were captured using the Bio-Rad ChemiDoc Touch imaging system.

Article Title: Host cellular protein RAB33B facilitates influenza viral replication and modulates M2 trafficking by enhancing autophagy.
Article Snippet: .. The transferred membranes were blocked at room temperature for 10 to 30 min with QuickBlock Blocking Buffer for western blotting (Beyotime Biotechnology, P0252), then incubated overnight at 4 °C with the appropriate primary antibodies. ..

Article Title: Lactoferrin prevents heat stroke-induced intestinal barrier damage by reducing ferroptosis via regulating MAPK signaling pathway: In vitro and in vivo studies.
Article Snippet: Samples were denatured and separated on 4–12 % SurePAGETM gels (GenScript, M00654) with 30 μg protein per lane, and proteins were transferred onto PVDF membranes using an eBlotTM L1 Fast Transfer System (GenScript, L00686C). .. Membranes were blocked with blocking buffer (Beyotime, P0252) for 1 h at room temperature, then incubated overnight with primary antibodies at 4 ◦C. ..

Article Title: Harnessing tissue-derived mitochondria-rich extracellular vesicles (Ti-mitoEVs) to boost mitochondrial biogenesis for regenerative medicine
Article Snippet: Equal particle numbers of EVs or equal amounts of EV protein were electrophoresed using sodium dodecyl sulfate–polyacrylamide gels, and then transferred to nitrocellulose membranes (Cytiva, Germany). .. The membranes were blocked in western blocking buffer (P0252, Beyotime) and incubated with anti-GM130 (A11408, ABClonal, Wuhan, China), anti-HSP70 (A0284, ABClonal), anti-TSG101 (14497-1-AP, Proteintech, Wuhan, China), anti-COXIV (11242-1-AP, Proteintech), anti-ATP5a (A5884, ABClonal), anti-VDAC (55259-1-AP, Proteintech), anti-TOM20 (no. 42406, Cell Signaling Technology, Beverly, MA, USA), anti-PDHB (14744-1-AP, Proteintech), and anti-pyruvate carboxylase (PC, 16588-1-AP, Proteintech) at 4°C overnight. ..

Article Title: Tankyrase activity is essential for asymmetric division and chromosome segregation in oocyte meiosis.
Article Snippet: The proteins were separated by electrophoresis on 10% SurePAGE (M00665, Nanjing GenScript Biotech Co., Nanjing City, China) at 140 V for 1 h, followed by transfer onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA) with eBlotTM L1 system (L00686C, Nanjing GenScript Biotech Co., Ltd., Nanjing City, China). .. The membranes were then blocked with QuickBlock Blocking Buffer (P0252, Beyotime BioTECH Co., Ltd., Shanghai, China) for 15 min at room temperature and subsequently incubated with anti- 14 β-Actin (1:2,000), anti-GAPDH (1:1,000), anti-Tubulin (1:2,000), anti-TNKS1 (1:500), anti-TNKS2 (1:500), anti-TNKS (1:200), anti-pT210-PLK1 (1:1,000), anti-Ac-Tubulin (1:2,000), anti-N-WASP (1:1,000), anti-Fascin (1:1,000), anti-ARP2 (1:1,000), antiRan (1:1,000), anti-pS19-Myosin II (1:1,000), anti-Rab11a (1:1,000), anti-Formin2 (1:1,000), anti-GRP78 (1:1,000), anti-Cyclin B1 (1:1,000), anti-Securin (1:1,000), antiCDC20 (1:500), anti-pT161 CDK1 (1:500), anti-pY15 CDK1 (1:500), anti-pT14 CDK1 (1:500), and anti-CDC25C (1:1,000) overnight at 4 °C. ..

Incubation:

Article Title: Trem2 regulates microglial migratory responses via type I interferon signaling during photoreceptor degeneration
Article Snippet: Ten micrograms of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (#1620177, Bio-Rad, Shanghai, China). .. Membranes were blocked with QuickBlockTM (#P0252, Beyotime) and incubated with primary and secondary antibodies (listed in Supplementary Table ). .. Protein detection was performed using Enhanced chemiluminescence (ECL) reagent, and bands were visualized using a ChemiDoc imaging system (#1705061, Bio-Rad).

Western Blot:

other:

Article Title: Immunopeptidomics-guided discovery and characterization of neoantigens for personalized cancer immunotherapy
Article Snippet: The NC membrane was blocked with rapid-blocking solution (Beyotime, catalog no. P0252, China) and incubated overnight at 4°C with primary antibodies against EFTUD2 (Proteintech, catalog no. 10208-1-AP, USA), NUP160 (Proteintech, catalog no. 16084-1-AP, USA), AKNA (Proteintech, catalog no. 23785-1-AP, USA), and β-actin (Proteintech, catalog no. 20536-1-AP, USA), followed by a 50-min incubation with secondary antibodies at room temperature.

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