p0260 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology p0260
P0260, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2002 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickblock+blocking+buffer+p0260/mouse+anti-goat+IgG-HRP/pmc05707017-283-22-26
Average 96 stars, based on 2002 article reviews

p0260 - by Bioz Stars, 2026-11

96/100 stars

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Cell-Signaling:

Article Title: CCNB1IP1 regulatory evolution underlies gradual increases in cortical size and folding in primates.
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies All primary antibodies were commercially obtained and used at the following dilutions: anti-Ki67 (Cell Signaling Technology, 9129S, rabbit monoclonal, 1:200); anti-GFP (Invitrogen, A10262, lot 2738237, chicken polyclonal, 1:500); anti-Pax6 (Invitrogen, 42-6600, rabbit polyclonal, 1:200); anti-TBR2/Eomes (Abcam, ab23345, rabbit polyclonal, 1:300); anti-SOX2 (Invitrogen, 53-9811-82, lot 3311132, rat monoclonal, 1:200); anti-TBR1 (Affinity Biosciences, DF2396, rabbit polyclonal, 1:200); anti-BRN2 (Santa Cruz Biotechnology, sc-393324, mouse monoclonal, 1:200);anti-NeuN (Millipore, ABN91, chicken polyclonal, 1:500); anti-SATB2 (Affinity Biosciences, DF2962, rabbit polyclonal, 1:200); anti-Ctip2 (Oasis Biofarm, OB-PGP012, guinea pig polyclonal, 1:200); anti-TBR2/ Eomes (Oasis Biofarm, OB-PGP022, lot N2204141-06, guinea pig polyclonal, 1:200); anti-Cyclin B1 (Cell Signaling Technology, 4138T,rabbit monoclonal, 1:1000); anti-CCNB1IP1 (Affinity Biosciences, DF4016,rabbit polyclonal, 1:500); anti-FLAG (Millipore, F1804,mouse monoclonal, 1:1000); anti-HA (Cell Signaling Technology, C29F4, rabbit monoclonal, 1:1000); anti-MYCN (Santa Cruz Biotechnology, sc-53993, mouse monoclonal, 1:200); anti-E2F1 (Santa Cruz Biotechnology, sc-251, rabbit polyclonal, 1:200); and antiα-Tubulin (Proteintech, 6603-1-Ig, mouse monoclonal, 1:5000). .. Secondary antibodies Secondary antibodies were used at the following dilutions: Alexa Fluor 594 Donkey Anti-Rabbit (Jackson ImmunoResearch, 711-585-152, polyclonal, 1:500); Alexa Fluor 488 Donkey Anti-Chicken (Jackson ImmunoResearch, 703-545-155, polyclonal, 1:500); Alexa Fluor 488 Donkey Anti-Rabbit (Jackson ImmunoResearch, 711-545-152, polyclonal, 1:500); Alexa Fluor 488 Donkey Anti-Rat (Jackson ImmunoResearch, 712-545-150, polyclonal, 1:500); Goat anti-Guinea pig IgG 594 (Oasis Biofarm, G-GP594, 1:500); Alexa Fluor 488 Donkey Anti-Mouse (Jackson ImmunoResearch, 715-545-151, polyclonal, 1:500); Alexa Fluor 594 Donkey Anti-Mouse (Jackson ImmunoResearch, 715-585-150, polyclonal, 1:500); Anti-rabbit IgG HRP-linked (Cell Signaling Technology, 7074, polyclonal, 1:2000); Anti-mouse IgG HRP-linked (Cell Signaling Technology, 7076, polyclonal, 1:2000); and Anti-Goat IgG HRP-linked (Santa Cruz Biotechnology, sc-2354, mouse monoclonal, 1:2000). ..

other:

Article Title: Progesterone-driven stabilization of hybrid E/M states in amniotic epithelial cells enhances regeneration and immune modulatory capacities
Article Snippet: Membranes were blocked with EveryBlot Blocking Buffer (#12010020; Bio-Rad Laboratories, Milan, Italy) for 5 minutes.

Immunopeptidomics:

Article Title: Lymphangiogenesis driven by VEGF-C reshapes tumor immune landscape and enables tumor eradication by viral immunotherapy in mouse melanoma models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa FluorTM 488 Goat anti-Chicken IgY (H + L) Thermo Fisher Scientific Cat# A-11039; RRID: AB_2534096 Alexa Fluor 488 F(ab’)2-goat anti-rabbit IgG Thermo Fisher Scientific Cat# A-11070; RRID: AB_2534114 Alexa Fluor 555 goat anti-rat IgG Thermo Fisher Scientific Cat# A-21434; RRID: AB_2535855 Alexa Fluor 594 Donkey anti-goat IgG Jackson ImmunoResearch Labs Cat# 705–586-147; RRID: AB_2340434 Anti-human VEGF-C R and D Systems Cat# AF752; RRID: AB_2241406 Anti-human VEGFR3 R and D Systems Cat# AF743; RRID: AB_355563 Anti-mouse CD8α, clone 53-6.7 Biolegend Cat# 100701; RRID: AB_312740 Anti-mouse CD4, clone RM4-5 Biolegend Cat# 100505; RRID: AB_312708 Anti-mouse/human CD11b, clone M1/70 Biolegend Cat# 101201; RRID: AB_312784 Anti-mouse F4/80, clone BM8 Biolegend Cat# 123102; RRID: AB_893504 Anti-mouse CD11c, clone N418 Biolegend Cat# 117301; RRID: AB_313770 Anti-mouse Ly-6C, clone HK1.4 Biolegend Cat# 128001; RRID: AB_1134213 Anti-mouse Ly-6G, clone 1A8 Biolegend Cat# 127601; RRID: AB_1089179 Anti-mouse LYVE-1 Angiobio Cat# 11–034; RRID: AB_2813732 Anti-mouse CD8α, clone D4W2Z Cell Signaling Technology Cat# 60168; RRID: AB_3699108 Anti-mouse CD4, clone D7D2Z Cell Signaling Technology Cat# 25229; RRID: AB_2798898 Anti-mouse Endomucin, clone V.7C7 Santa Cruz Biotechnology Cat# sc-65495; RRID: AB_2100037 Anti-mouse CD45 Abcam Cat# ab10558; RRID: AB_442810 Anti-mouse Iba1 FUJIFILM Wako Pure Chemical Corporation Cat# 019–19741; RRID: AB_839504 Anti-mouse CD45R PE-CF594, clone RA3-6B2 BD Biosciences Cat# 562313; RRID: AB_11154399 Anti-mouse CD192 (CCR2) APC/FireTM 750, clone SA203G11 Biolegend Cat# 150629; RRID: AB_2810416 Anti-mouse CD103 PE, clone 2E7 Biolegend Cat# 121405; RRID: AB_535948 Anti-mouse/human CD11b APC, clone M1/70 Biolegend Cat# 101211; RRID: AB_312794 Anti-mouse CD11c PerCP, clone N418 Biolegend Cat# 117325; RRID: AB_893236 Anti-mouse CD25 APC, clone PC61 Biolegend Cat# 102011; RRID: AB_312860 Anti-mouse CD28 Alexa Fluor 488, clone E18 Biolegend Cat# 122017; RRID: AB_604065 Anti-human CD3 BV750, clone SK7 BD Bioscience Cat# 747058; RRID: AB_2871816 Anti-mouse CD4 PerCP-eFluorTM 710, clone GK1.5 Thermo Fisher Scientific Cat# 46–0041-82; RRID: AB_11150050 Anti-mouse/human CD44 APC/FireTM 750, clone IM7 Biolegend Cat# 103061; RRID: AB_2616726 Anti-mouse CD45 BV480, clone 30-F11 BD Biosciences Cat# 566168; RRID: AB_2739565 Anti-mouse CD49b Alexa Fluor 647, clone HMα2 Biolegend Cat# 103511; RRID: AB_528830 Anti-mouse CD62L Brilliant Violet 421TM, clone MEL-14 Biolegend Cat# 104435; RRID: AB_10900082 Anti-mouse CD64 PerCP/Cy5.5, clone X545/7.1 Biolegend Cat# 139307; RRID: AB_2561962 (Continued on next page) Cell Reports 45, 118130, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse CD8a Brilliant Violet 510TM, clone 53-6.7 Biolegend Cat# 100751; RRID: AB_2561389 Anti-mouse CD80 V450, clone 16-10A1 BD Biosciences Cat# 560523; RRID: AB_1727515 Anti-mouse CD83 Brilliant Violet 650TM, clone Michel-19 Biolegend Cat# 121515; RRID: AB_11203713 Anti-mouse CD86 FITC, clone GL-1 Biolegend Cat# 105005; RRID: AB_313148 Anti-mouse CD152 APC-R700, clone UC10-4F10-11 BD Biosciences Cat# 565778; RRID: AB_2739350 Anti-mouse CD183 BV650,clone CXCR3-173 BD Biosciences Cat# 740630; RRID: AB_2740325 Anti-mouse F4/80 Brilliant Violet 421TM, clone BM8 Biolegend Cat# 123131; RRID: AB_10901171 Anti-mouse Foxp3 V450, clone MF23 BD Biosciences Cat# 561293; RRID: AB_10611728 Anti-human/mouse Granzyme B FITC, clone GB11 Biolegend Cat# 515403; RRID: AB_2114575 Anti-mouse IFN-γ Brilliant Violet 711TM, clone XMG1.2 Biolegend Cat# 505835; RRID: AB_11219588 Anti-mouse KLRG1 V450, clone 2F1 BD Biosciences Cat# 561625; RRID: AB_10892825 Anti-mouse Ly-6C Alexa Fluor 700, clone HK1.4 Biolegend Cat# 128023; RRID: AB_10640119 Anti-mouse Ly-6G PE/Cy7, clone 1A8 Biolegend Cat# 127617; RRID: AB_1877262 Anti-mouse MHCII Brilliant Violet 785TM, clone M5/114.15.2 Biolegend Cat# 107645; RRID: AB_2565977 Anti-mouse NK-1.1 Brilliant Violet 650TM, clone PK136 Biolegend Cat# 108735; RRID: AB_11147949 Anti-mouse CD274 (PD-L1) BV711, clone 10F.9G2 BD Biosciences Cat# 568562; RRID: AB_3684373 Anti-mouse CD279 (PD-1) Brilliant Violet 605TM, clone 29F.1A12 Biolegend Cat# 135219; RRID: AB_11125371 Anti-mouse TNF BB700, clone MP6-XT22 BD Biosciences Cat# 566510; RRID: AB_2869775 Anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2354; RRID: AB_628490 Anti-mouse CD11c PerCP-Cy5.5 Biolegend Cat# 117328; RRID: AB_2129641 Anti-mouse CD11b PE BD Biosciences Cat# 553311; RRID: AB_394775 Anti-mouse NKp46 PE-Dazzle594 Biolegend Cat# 137630; RRID: AB_2616666 Anti-mouse Ly6G PE-Cy7 Biolegend Cat# 127618; RRID: AB_1877261 Anti-mouse MHC-II eFluor450 Thermo Fisher Scientific Cat# 48–5321-82; RRID: AB_1272204 Anti-mouse CD4 BV510 BD Biosciences Cat# 563106; RRID: AB_2687550 Anti-mouse Ly6C BV605 BD Biosciences Cat# 563011; RRID: AB_2737949 Anti-mouse CD3 BV650 BD Biosciences Cat# 564378; RRID: AB_2738779 Anti-mouse CD45 BV750 BD Biosciences Cat# 746947; RRID: AB_2871734 Bacterial and virus strains NDV LaSota-L289A Adolfo Garcia-Sastre Lab N/A APMV-4 Duck/Hong Kong/D3/1975 Adolfo Garcia-Sastre Lab N/A Critical commercial assays Amicon Ultra-15 Centrifugal Filter Unit Sigma Millipore Cat# UFC900324 iScriptTM cDNA Synthesis Kit Biorad Cat# 1708890 Human VEGF-C Quantikine ELISA Kit R and D Systems Cat# DVEC00 Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA R and D Systems Cat# DY466 RNeasy mini KIT QIAGEN Cat# 74104 (Continued on next page) 20 Cell Reports 45, 118130, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glass slides ICN Biomedical Cat# 6041805 Mouse Tumor Dissociation Kit Miltenyi Cat# 130–096-730 FOXP3 Transcription Factor Staining Buffer Set eBioscience Cat# 00–5523-00 UltraComp beads eBioscience Cat# 01–2222-41 Fixable Yellow Dead Cell Stain Kit Invitrogen Cat# L34968 BondTM 10X Wash Solution Leica Biosystems Cat# AR9590 BondTM Dewax Solution Leica Biosystems Cat# AR9222 BondTM Epitope Retrieval 1 (citrate, pH6) Leica Biosystems Cat# AR9961 BondTM Polymer Refine Detection System Leica Biosystems Cat# DS9800 BondTM Polymer Refine Red Detection System Leica Biosystems Cat# DS9390 BondTM Primary Antibody Diluent Leica Biosystems Cat# AR9352 Deposited data TCGA-SKCM RNA-seq data NIH/NCI RRID: SCR_003193 Code in Github Github https://github.com/rkarlic/vegfc-ndv- analysis Code in Zenodo Zenodo https://doi.org/10.5281/zenodo.22713153 Experimental models: Cell lines B16F10 - EV Melody Swartz Lab RRID: CVCL_0159 B16F10 - VEGF-C Melody Swartz Lab RRID: CVCL_0159 M4 (B2905) Glenn Merlino Lab RRID: CVCL_B0CG M4 - EV Mihaela Skobe Lab RRID: CVCL_B0CG M4 - VEGF-C Mihaela Skobe Lab RRID: CVCL_B0CG Vero Adolfo Garcia-Sastre Lab RRID: CVCL_0059 Experimental models: Organisms/strains C57Bl/6J The Jackson Laboratory RRID: IMSR_JAX:000664 C57Bl/6N Charles River RRID: IMSR_CRL:027 Oligonucleotides Primers for RT-qPCR, see Table S4 This paper N/A Recombinant DNA pD2109-EV ATUMbio pD2109-CMV-03 pD2109- VEGF-C ATUMbio N/A pMD2.G Addgene RRID:Addgene_12259 psPAX2 Addgene RRID:Addgene_12260 VB210401 Vector Builder N/A Software and algorithms FCS Express (v. 7.0) De Novo Software RRID: SCR_016431 Fiji (1.53q) NIH RRID: SCR_002285 NDP viewer 2 Hamamatsu https://www.hamamatsu.com/ GraphPad Prism v.11 Dotmatic RRID: SCR_002798 QuPath N/A RRID: SCR_018257 FlowJo V10.10.0 N/A RRID: SCR_008520 R statistical environment Free Software Foundation RRID: SCR_001905 Other Aurora Spectral Analyzer Cytek Bioscience RRID: SCR_019826 Attune CytPix Flow Cytometer Thermoscientific RRID: SCR_028356 StepOneTM Real-Time PCR System Applied Biosystems RRID: SCR_023455 (Continued on next page) Cell Reports 45, 118130, October 27, 2026 21

Virus:

cDNA Synthesis:

Enzyme-linked Immunosorbent Assay:

Incubation:

Article Title: Loss of WT1 Drives Adaptive Plasticity in CCDC6-RET Selpercatinib-Resistant Papillary Thyroid Cancer
Article Snippet: The protein transfer onto membranes was carried out using Trans-Blot Turbo Transfer Packs (Bio-Rad, Hercules, CA, USA) for 3 min at 2.5 A and 25 V with the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA). .. The membranes were washed in T-TBS and incubated for 1 h at room temperature with the following secondary antibodies: mouse anti-rabbit IgG-HRP (sc-2357, Santa Cruz Biotechnology), mouse anti-goat IgG-HRP (sc2354, Santa Cruz Biotechnology), m-IgGk BP-HRP (sc-516102, Santa Cruz Biotechnology). .. After blocking in Tris-buffered saline containing 0.05% Tween-20 (T-TBS) supplemented with 1× PierceTM Clear Milk Blocking Buffer (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at room temperature.

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