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5x nebnext quick ligation reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 5x nebnext quick ligation reaction buffer
    5x Nebnext Quick Ligation Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quick+ligation+buffer/NEBNext+Quick+Ligation+RxBuff/pm41916977-384-23-24
    Average 98 stars, based on 597 article reviews
    5x nebnext quick ligation reaction buffer - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Ligation:

    Article Title: Aminoacylated tRNA tails trigger auxiliary bacterial immunity
    Article Snippet: .. For each sample, 300 ng polyadenylated RNA was ligated to 1 μl of 0.7 μM annealed RTA in a 10-μl reaction containing 2 μl 5× NEBNext Quick Ligation Reaction Buffer (New England Biolabs), 0.5 μl high-concentration T4 DNA ligase (New England Biolabs) and 0.2 μl RNasin. ..

    Article Title: Escape from replicative crisis and immortalization precedes complex genome evolution
    Article Snippet: .. Next, 5 μl of Native Adapter (ONT, EXP-NBA114) was ligated to 65 μl of the sample using 10 μl Salt-T4 DNA ligase in 1x NEBNext Quick ligation reaction buffer (NEB, B6058) at RT for 20 min followed by purification with 50 μl AMPure XP beads (0.5x v/v), as described above using 33 μl elution buffer (ONT) to elute the library. ..

    Article Title: Dynamic tRNA modification programs align codon usage with growth-phase-specific gene expression in Pseudomonas aeruginosa .
    Article Snippet: .. Finally, ONT RML sequencing adapters (DRS Kit, SQK- RNA004, Oxford Nanopore Technologies) were ligated to tRNAs at room temperature for 30 min in a total reaction volume of 40 μL [1× Quick Ligation Reaction buffer (NEB, B6058S), 3 μL of T4 DNA Ligase (NEB, M0202M, 2,000,000 μ/mL), 6 μL of ONT RMX adapters]. ..

    Article Title: Direct RNA sequencing-based multi-omics profiling reveals transcriptional and post-transcriptional remodeling in mice with diet-induced atherosclerosis
    Article Snippet: .. Briefly, 9 μL poly(A)+ RNA was combined with 3 μL NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. #B6058, United States), 1 μL RT Adapter (Oxford Nanopore Technologies, cat. #SQK-RNA004, United Kingdom), and 2 μL T4 DNA Ligase (New England Biolabs, cat. #M0202, United States), followed by incubation at 25 °C for 10 min to ligate RNA adapters. .. For reverse transcription, 8 μL of 5 × first-strand buffer (New England Biolabs, cat. #M0681, United States), 2 μL of 10 mM Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, cat. #N0447, United States), 9 μL of nuclease-free water, 4 μL of 0.1 M dithiothreitol, and 2 μL Induro Reverse Transcriptase (New England Biolabs, cat. #M0681, United States) were added, followed by incubation at 50 °C for 50 min and at 70 °C for 10 min.

    Article Title: Protocol for rolling circle amplification-based nanopore sequencing of eccDNA from cell and plasma samples.
    Article Snippet: .. The pooled barcoded sample 65 μL Native Adapter (NA) 5 μL NEBNext Quick Ligation Reaction Buffer (53) 20 μL Quick T4 DNA Ligase 10 μL Total 100 μL 18 STAR Protocols 7, 104865, December 18, 2026 ..

    Article Title: A direct RNA sequencing workflow for simultaneous profiling of tRNA abundance and modifications in mouse liver
    Article Snippet: Barcoded samples were pooled to 20 ng of total nucleic acid in 23 μL (equal amounts of up to six samples, quantified with the Qubit RNA Assay Kit, Thermo Fisher). .. The RNA ligation adapter was ligated in a 40 μL reaction containing NEBNext Quick Ligation Reaction Buffer, 6 μL of RNA Ligation Adapter (SQK-RNA004), and 3 μL of T4 DNA Ligase, at room temperature for 10 min. .. The library was purified with Agencourt RNA Clean XP beads (Beckman Coulter, A63987), washed, and eluted in 13 μL of RNA Elution Buffer.

    Article Title: Metagenomic analysis reveals recombinant Deformed Wing Viruses in winter-collapsed colonies
    Article Snippet: Equal amounts of barcoded DNA from each sample were pooled in a single Eppendorf DNA Lobind Tube (Eppendorf, Hamburg, Germany). .. Sequencing adapters were added to the combined sample using NEBNext Quick T4 DNA Ligase and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA). .. All DNA purification steps were performed using Agencourt AMPure XP beads (Beckman Coulter, Indianapolis, IN).

    Article Title: HIV-1 Infection Alters Post-Transcriptional Processing of HTLV-1 RNA in MT-2 Cells
    Article Snippet: Nanopore direct RNA sequencing libraries were prepared from 1 μg of total RNA using the Direct RNA Sequencing Kit (SQK-RNA004; Oxford Nanopore Technologies, Oxford, UK) according to the manufacturer’s protocol, with minor modifications [45–48]. .. Briefly, 1 μg of total RNA in 8 μL was ligated to the RT Adapter in a 15-μL reaction containing 3 μL of NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA; B6058), 1 μL of RNaseOUT Recombinant Ribonuclease Inhibitor, 1 μL of RT Adapter, 1.5 μL of T4 DNA Ligase (New England Biolabs; M0202), and 0.5 μL of nuclease-free water. .. The reaction was incubated at room temperature for 20 min. First-strand cDNA synthesis was performed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA; 18080044) at 50 ◦C for 50 min, followed by enzyme inactivation at 70 ◦C for 10 min.

    Purification:

    Article Title: Escape from replicative crisis and immortalization precedes complex genome evolution
    Article Snippet: .. Next, 5 μl of Native Adapter (ONT, EXP-NBA114) was ligated to 65 μl of the sample using 10 μl Salt-T4 DNA ligase in 1x NEBNext Quick ligation reaction buffer (NEB, B6058) at RT for 20 min followed by purification with 50 μl AMPure XP beads (0.5x v/v), as described above using 33 μl elution buffer (ONT) to elute the library. ..

    Sequencing:

    Article Title: Dynamic tRNA modification programs align codon usage with growth-phase-specific gene expression in Pseudomonas aeruginosa .
    Article Snippet: .. Finally, ONT RML sequencing adapters (DRS Kit, SQK- RNA004, Oxford Nanopore Technologies) were ligated to tRNAs at room temperature for 30 min in a total reaction volume of 40 μL [1× Quick Ligation Reaction buffer (NEB, B6058S), 3 μL of T4 DNA Ligase (NEB, M0202M, 2,000,000 μ/mL), 6 μL of ONT RMX adapters]. ..

    Article Title: Metagenomic analysis reveals recombinant Deformed Wing Viruses in winter-collapsed colonies
    Article Snippet: Equal amounts of barcoded DNA from each sample were pooled in a single Eppendorf DNA Lobind Tube (Eppendorf, Hamburg, Germany). .. Sequencing adapters were added to the combined sample using NEBNext Quick T4 DNA Ligase and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA). .. All DNA purification steps were performed using Agencourt AMPure XP beads (Beckman Coulter, Indianapolis, IN).

    Incubation:

    Article Title: Direct RNA sequencing-based multi-omics profiling reveals transcriptional and post-transcriptional remodeling in mice with diet-induced atherosclerosis
    Article Snippet: .. Briefly, 9 μL poly(A)+ RNA was combined with 3 μL NEBNext Quick Ligation Reaction Buffer (New England Biolabs, cat. #B6058, United States), 1 μL RT Adapter (Oxford Nanopore Technologies, cat. #SQK-RNA004, United Kingdom), and 2 μL T4 DNA Ligase (New England Biolabs, cat. #M0202, United States), followed by incubation at 25 °C for 10 min to ligate RNA adapters. .. For reverse transcription, 8 μL of 5 × first-strand buffer (New England Biolabs, cat. #M0681, United States), 2 μL of 10 mM Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, cat. #N0447, United States), 9 μL of nuclease-free water, 4 μL of 0.1 M dithiothreitol, and 2 μL Induro Reverse Transcriptase (New England Biolabs, cat. #M0681, United States) were added, followed by incubation at 50 °C for 50 min and at 70 °C for 10 min.

    Recombinant:

    Article Title: HIV-1 Infection Alters Post-Transcriptional Processing of HTLV-1 RNA in MT-2 Cells
    Article Snippet: Nanopore direct RNA sequencing libraries were prepared from 1 μg of total RNA using the Direct RNA Sequencing Kit (SQK-RNA004; Oxford Nanopore Technologies, Oxford, UK) according to the manufacturer’s protocol, with minor modifications [45–48]. .. Briefly, 1 μg of total RNA in 8 μL was ligated to the RT Adapter in a 15-μL reaction containing 3 μL of NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA; B6058), 1 μL of RNaseOUT Recombinant Ribonuclease Inhibitor, 1 μL of RT Adapter, 1.5 μL of T4 DNA Ligase (New England Biolabs; M0202), and 0.5 μL of nuclease-free water. .. The reaction was incubated at room temperature for 20 min. First-strand cDNA synthesis was performed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA; 18080044) at 50 ◦C for 50 min, followed by enzyme inactivation at 70 ◦C for 10 min.



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