Review




Structured Review

Promega quick ligasing buffer
Quick Ligasing Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quick+ligase+buffer/quick+ligasing+buffer/pmc02863166-173-41-44
Average 90 stars, based on 1 article reviews
quick ligasing buffer - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Mutagenesis:

Article Title: cAMP/CREB-mediated Transcriptional Regulation of Ectonucleoside Triphosphate Diphosphohydrolase 1 (CD39) Expression
Article Snippet: The 4555-bp insert was subcloned into pCR-XL-TOPO vector (Invitrogen) using the TOPO XL PCR cloning kit (Invitrogen) and sequenced to confirm identity. .. Construction of Mouse Cd39 Promoter-Luciferase Reporter Plasmids and Site-directed Mutagenesis PCR was used to amplify mouse Cd39 promoter-luciferase reporter constructs representing 5′-deleted Cd39 upstream sequences, and these PCR products were subcloned into the pGL3-basic vector (Promega) using a T4 ligase and Quick Ligasing buffer (Promega). ..

Polymerase Chain Reaction:

Article Title: cAMP/CREB-mediated Transcriptional Regulation of Ectonucleoside Triphosphate Diphosphohydrolase 1 (CD39) Expression
Article Snippet: The 4555-bp insert was subcloned into pCR-XL-TOPO vector (Invitrogen) using the TOPO XL PCR cloning kit (Invitrogen) and sequenced to confirm identity. .. Construction of Mouse Cd39 Promoter-Luciferase Reporter Plasmids and Site-directed Mutagenesis PCR was used to amplify mouse Cd39 promoter-luciferase reporter constructs representing 5′-deleted Cd39 upstream sequences, and these PCR products were subcloned into the pGL3-basic vector (Promega) using a T4 ligase and Quick Ligasing buffer (Promega). ..

Article Title: cAMP/CREB-mediated Transcriptional Regulation of Ectonucleoside Triphosphate Diphosphohydrolase 1 (CD39) Expression
Article Snippet: The 4555-bp insert was subcloned into pCR-XL-TOPO vector (Invitrogen) using the TOPO XL PCR cloning kit (Invitrogen) and sequenced to confirm identity. .. PCR was used to amplify mouse Cd39 promoter-luciferase reporter constructs representing 5′-deleted Cd39 upstream sequences, and these PCR products were subcloned into the pGL3-basic vector (Promega) using a T4 ligase and Quick Ligasing buffer (Promega). ..

Construct:

Article Title: cAMP/CREB-mediated Transcriptional Regulation of Ectonucleoside Triphosphate Diphosphohydrolase 1 (CD39) Expression
Article Snippet: The 4555-bp insert was subcloned into pCR-XL-TOPO vector (Invitrogen) using the TOPO XL PCR cloning kit (Invitrogen) and sequenced to confirm identity. .. Construction of Mouse Cd39 Promoter-Luciferase Reporter Plasmids and Site-directed Mutagenesis PCR was used to amplify mouse Cd39 promoter-luciferase reporter constructs representing 5′-deleted Cd39 upstream sequences, and these PCR products were subcloned into the pGL3-basic vector (Promega) using a T4 ligase and Quick Ligasing buffer (Promega). ..

Article Title: cAMP/CREB-mediated Transcriptional Regulation of Ectonucleoside Triphosphate Diphosphohydrolase 1 (CD39) Expression
Article Snippet: The 4555-bp insert was subcloned into pCR-XL-TOPO vector (Invitrogen) using the TOPO XL PCR cloning kit (Invitrogen) and sequenced to confirm identity. .. PCR was used to amplify mouse Cd39 promoter-luciferase reporter constructs representing 5′-deleted Cd39 upstream sequences, and these PCR products were subcloned into the pGL3-basic vector (Promega) using a T4 ligase and Quick Ligasing buffer (Promega). ..

Plasmid Preparation:

Article Title: cAMP/CREB-mediated Transcriptional Regulation of Ectonucleoside Triphosphate Diphosphohydrolase 1 (CD39) Expression
Article Snippet: The 4555-bp insert was subcloned into pCR-XL-TOPO vector (Invitrogen) using the TOPO XL PCR cloning kit (Invitrogen) and sequenced to confirm identity. .. Construction of Mouse Cd39 Promoter-Luciferase Reporter Plasmids and Site-directed Mutagenesis PCR was used to amplify mouse Cd39 promoter-luciferase reporter constructs representing 5′-deleted Cd39 upstream sequences, and these PCR products were subcloned into the pGL3-basic vector (Promega) using a T4 ligase and Quick Ligasing buffer (Promega). ..

Article Title: cAMP/CREB-mediated Transcriptional Regulation of Ectonucleoside Triphosphate Diphosphohydrolase 1 (CD39) Expression
Article Snippet: The 4555-bp insert was subcloned into pCR-XL-TOPO vector (Invitrogen) using the TOPO XL PCR cloning kit (Invitrogen) and sequenced to confirm identity. .. PCR was used to amplify mouse Cd39 promoter-luciferase reporter constructs representing 5′-deleted Cd39 upstream sequences, and these PCR products were subcloned into the pGL3-basic vector (Promega) using a T4 ligase and Quick Ligasing buffer (Promega). ..



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