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LGC Biosearch unc-5 probe with quasar 570 dye
Unc 5 Probe With Quasar 570 Dye, supplied by LGC Biosearch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quasar+program/egfp+mrna+probes+conjugated+to+quasar+670+dye+vsmf+1015+5/pm36563525-265-89-92
Average 90 stars, based on 1 article reviews
unc-5 probe with quasar 570 dye - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Protocol for single-molecule FISH in the developing mouse retinal vasculature.
Article Snippet: See Table S1 LGC Biosearch Technologies SMF-1063-5 smFISH probe: pre-designed Gapdh Quasar 670, 1 nmol LGC Biosearch Technologies SMF-3140-1 (Continued on next page) STAR Protocols 5, 103510, December 20, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Fiji Schindelin et al.6 https://imagej.net/software/fiji/downloads Imaris Oxford Instruments https://imaris.oxinst.com/ RS-FISH Bahry et al.7 https://github.com/PreibischLab/RS-FISH) Other Confocal microscope Leica Microsystems Stellaris 5 Dissection microscope Olympus SZX7 See-saw shaker Stuart SSL4 Hybridization oven/shaker Amersham Biosciences RPN2511 ll OPEN ACCESS Protocol MATERIALS AND EQUIPMENT 23 SSC buffer Reagent Final concentration Amount 203 SSC Buffer 23 5 mL Nuclease-Free water N/A 45 mL Total volume N/A 50 mL Prepare fresh 23 SSC buffer for each round of experiment.

Article Title: Protocol for single-molecule FISH in the developing mouse retinal vasculature
Article Snippet: smFISH probe: pre-designed Gapdh Quasar 670, 1 nmol , LGC Biosearch Technologies , SMF-3140-1.

Article Title: Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription
Article Snippet: Embryos were fixed in 10% formaldehyde/heptane under blue light or in the dark as appropriate for 25 min with shaking before a methanol quench and stored at −20°C in methanol before use. smFISH probes targeting twi conjugated to Quasar-580 (LGC Biosearch Technologies) were designed and used as previously described ( ). smFISH probes targeting the 5′ region of the sog transcript were designed using Oligostan (Integrated DNA Technologies) ( ).

Labeling:

Article Title: DEAD box 1 (DDX1) protein binds to and protects cytoplasmic stress response mRNAs in cells exposed to oxidative stress.
Article Snippet: .. BRCA1 and DDX3X probe sets were labeled with CALFluor Red 590 and purchased from LGC Biosearch Technologies together with the predesigned smFISH probe set against GAPDH (SMF-2026). ..

Article Title: M 6 A reduction relieves FUS-associated ALS granules.
Article Snippet: For the quantification of “FUS-only” granules in recovery experiments, the G3BP1 signal was used to create a mask and then removed from the FUS signal before the quantification of the number and volumes of FUS-only granules using the ImageJ tool “3D Object Counter”. smFISH FISH Probes against CALM1, HUWE1, and CLSNT1 transcripts were designed by utilizing the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, see Supplementary Data 4) and labeled with ddUTP-Atto633 (AD 633-31 and ATTO-TEC) following ref. 49. .. FISH probes for GAPDH mRNA, labeled with Quasar 570 Dye (SMF-2026-1), were purchased from LGC Biosearch Technologies. ..

Fluorescence In Situ Hybridization:

Article Title: M 6 A reduction relieves FUS-associated ALS granules.
Article Snippet: For the quantification of “FUS-only” granules in recovery experiments, the G3BP1 signal was used to create a mask and then removed from the FUS signal before the quantification of the number and volumes of FUS-only granules using the ImageJ tool “3D Object Counter”. smFISH FISH Probes against CALM1, HUWE1, and CLSNT1 transcripts were designed by utilizing the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, see Supplementary Data 4) and labeled with ddUTP-Atto633 (AD 633-31 and ATTO-TEC) following ref. 49. .. FISH probes for GAPDH mRNA, labeled with Quasar 570 Dye (SMF-2026-1), were purchased from LGC Biosearch Technologies. ..



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