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Promega quantustm fluorometer

Quantustm Fluorometer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantustm+e6150+fluorometer/quantustm+fluorometer/pmc09356155-48-0-3
Average 90 stars, based on 1 article reviews
quantustm fluorometer - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "Protocol to estimate cell type proportions from bulk RNA-seq using DAISM-DNN XMBD"

Article Title: Protocol to estimate cell type proportions from bulk RNA-seq using DAISM-DNN XMBD

Journal: STAR Protocols

doi: 10.1016/j.xpro.2022.101587


Figure Legend Snippet:

Techniques Used: Recombinant, Saline, Flow Cytometry, Software, Sterility

Related Articles

Polymerase Chain Reaction:

Article Title: Insights into the interlinkages between rhizosphere soil extracellular enzymes and microbiome assemblages across soil profiles in grasslands
Article Snippet: Understanding the correlations between the turfgrass rhizosphere soil (RS) microbial community assembly and soil extracellular enzymes (EEs) across soil profiles can contribute to enhancing the soil C pool and improving urban grassland ecosystem services.. This study collected rhizosphere soil from non-native Poa pratensis (Poa L.) and native Carex leucochlora (Carex L.) at depths of 0–40 cm to investigate soil properties, EEs, and microbial communities.. Compared to Poa, Carex significantly increased RS soil properties (i.e., organic carbon (SOC), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and available NPK), C-, and Nacquisition (acq) enzymes, and soil ecosystem multifunction at 0–40 cm soil depths.

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Article Title: Spatiotemporal characterization of the dynamic changes in the intestinal microbiota of Taihe Silky Fowl.
Article Snippet: As instructed by the manufacturer, the PCR products were extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, CA, USA). .. The purified PCR products were subsequently quantified using a QuantusTM fluorometer (Promega, USA). .. After quantification, the purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina NextSeq 2000 platform (Illumina, San Diego, USA) according to the standard protocols of Majorbio Tech.

Purification:

Article Title: Insights into the interlinkages between rhizosphere soil extracellular enzymes and microbiome assemblages across soil profiles in grasslands
Article Snippet: Understanding the correlations between the turfgrass rhizosphere soil (RS) microbial community assembly and soil extracellular enzymes (EEs) across soil profiles can contribute to enhancing the soil C pool and improving urban grassland ecosystem services.. This study collected rhizosphere soil from non-native Poa pratensis (Poa L.) and native Carex leucochlora (Carex L.) at depths of 0–40 cm to investigate soil properties, EEs, and microbial communities.. Compared to Poa, Carex significantly increased RS soil properties (i.e., organic carbon (SOC), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and available NPK), C-, and Nacquisition (acq) enzymes, and soil ecosystem multifunction at 0–40 cm soil depths.

Article Title: Flavonoids of Mao Jian Green Tea Ameliorate Glycemic Metabolism in Type-2-Diabetic Rats via AMPK Signaling Pathways and Gut Microbiota Regulation.
Article Snippet: The hypervariable V3-V4 regions of bacterial 16S rRNA genes were amplified via PCR (ABI GeneAmp® 9700; Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) employing universal primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′- GGACTACHVGGGTWTCTAAT-3′). .. Amplicons were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc., Union City, CA, USA) and quantified with a QuantusTM Fluorometer (Promega (Beijing) Biotech Co., Ltd., Beijing, China). .. Sequencing libraries were constructed with the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific Corporation, Austin, TX, USA), and paired-end sequencing (2 × 300 bp) was performed on an Illumina MiSeq platform (Majorbio Bio-Pharm Technology Co., Shanghai, China).

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Article Title: The impact of rainfall changes on soil bacterial and fungal communities in Pinus yunnanensis
Article Snippet: PCR products from the same sample were mixed and recovered on a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), detected by electrophoresis on a 2% agarose gel, and quantified by QuantusTM Fluorometer (Promega, USA). .. The recovered products were purified by 2% agarose gel electrophoresis, detected and quantified by QuantusTM Fluorometer (Promega, USA). .. Library construction was performed using the VAHTS® Universal Plus DNA Library Prep Kit for MGI V2 (Vazyme Biotech Co., Ltd, China) on purified PCR products. (1) linker link; (2) Use magnetic beads to screen and remove adapter self-connecting fragments; (3) Enrichment of library templates using PCR amplification; (4) Magnetic bead recycling of PCR products; and (5) PCR product cyclization.

Article Title: Spatiotemporal characterization of the dynamic changes in the intestinal microbiota of Taihe Silky Fowl.
Article Snippet: As instructed by the manufacturer, the PCR products were extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, CA, USA). .. The purified PCR products were subsequently quantified using a QuantusTM fluorometer (Promega, USA). .. After quantification, the purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina NextSeq 2000 platform (Illumina, San Diego, USA) according to the standard protocols of Majorbio Tech.

Gel Extraction:

Article Title: Insights into the interlinkages between rhizosphere soil extracellular enzymes and microbiome assemblages across soil profiles in grasslands
Article Snippet: Understanding the correlations between the turfgrass rhizosphere soil (RS) microbial community assembly and soil extracellular enzymes (EEs) across soil profiles can contribute to enhancing the soil C pool and improving urban grassland ecosystem services.. This study collected rhizosphere soil from non-native Poa pratensis (Poa L.) and native Carex leucochlora (Carex L.) at depths of 0–40 cm to investigate soil properties, EEs, and microbial communities.. Compared to Poa, Carex significantly increased RS soil properties (i.e., organic carbon (SOC), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and available NPK), C-, and Nacquisition (acq) enzymes, and soil ecosystem multifunction at 0–40 cm soil depths.

Article Title: Flavonoids of Mao Jian Green Tea Ameliorate Glycemic Metabolism in Type-2-Diabetic Rats via AMPK Signaling Pathways and Gut Microbiota Regulation.
Article Snippet: The hypervariable V3-V4 regions of bacterial 16S rRNA genes were amplified via PCR (ABI GeneAmp® 9700; Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) employing universal primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′- GGACTACHVGGGTWTCTAAT-3′). .. Amplicons were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc., Union City, CA, USA) and quantified with a QuantusTM Fluorometer (Promega (Beijing) Biotech Co., Ltd., Beijing, China). .. Sequencing libraries were constructed with the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific Corporation, Austin, TX, USA), and paired-end sequencing (2 × 300 bp) was performed on an Illumina MiSeq platform (Majorbio Bio-Pharm Technology Co., Shanghai, China).

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

other:

Article Title: An acidic polysaccharide from Lycium barbarum L: Isolation, purification, structural characterization, and therapeutic effects on ulcerative colitis.
Article Snippet: Addressing microbial imbalance is a critical therapeutic strategy for ulcerative colitis (UC).. Natural polysaccharides have attracted increasing attention for their potential in UC prevention.. In this study, we purified and characterized an acidic heteropolysaccharide (LBP 2–1) from Lycium barbarum L. and systematically evaluated its therapeutic effects on UC.

Article Title: Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR
Article Snippet: The concentration of the final DNA library was determined by Quantus Fluorometer (Promega) and ranged between 19 and 64 nM.

DNA Sequencing:

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Amplification:

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Agarose Gel Electrophoresis:

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Article Title: The impact of rainfall changes on soil bacterial and fungal communities in Pinus yunnanensis
Article Snippet: PCR products from the same sample were mixed and recovered on a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), detected by electrophoresis on a 2% agarose gel, and quantified by QuantusTM Fluorometer (Promega, USA). .. The recovered products were purified by 2% agarose gel electrophoresis, detected and quantified by QuantusTM Fluorometer (Promega, USA). .. Library construction was performed using the VAHTS® Universal Plus DNA Library Prep Kit for MGI V2 (Vazyme Biotech Co., Ltd, China) on purified PCR products. (1) linker link; (2) Use magnetic beads to screen and remove adapter self-connecting fragments; (3) Enrichment of library templates using PCR amplification; (4) Magnetic bead recycling of PCR products; and (5) PCR product cyclization.



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