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Promega quantustm fluorometer
Quantustm Fluorometer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

Polymerase Chain Reaction:

Article Title: Insights into the interlinkages between rhizosphere soil extracellular enzymes and microbiome assemblages across soil profiles in grasslands
Article Snippet: Understanding the correlations between the turfgrass rhizosphere soil (RS) microbial community assembly and soil extracellular enzymes (EEs) across soil profiles can contribute to enhancing the soil C pool and improving urban grassland ecosystem services.. This study collected rhizosphere soil from non-native Poa pratensis (Poa L.) and native Carex leucochlora (Carex L.) at depths of 0–40 cm to investigate soil properties, EEs, and microbial communities.. Compared to Poa, Carex significantly increased RS soil properties (i.e., organic carbon (SOC), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and available NPK), C-, and Nacquisition (acq) enzymes, and soil ecosystem multifunction at 0–40 cm soil depths.

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Article Title: Spatiotemporal characterization of the dynamic changes in the intestinal microbiota of Taihe Silky Fowl.
Article Snippet: As instructed by the manufacturer, the PCR products were extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, CA, USA). .. The purified PCR products were subsequently quantified using a QuantusTM fluorometer (Promega, USA). .. After quantification, the purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina NextSeq 2000 platform (Illumina, San Diego, USA) according to the standard protocols of Majorbio Tech.

Purification:

Article Title: Insights into the interlinkages between rhizosphere soil extracellular enzymes and microbiome assemblages across soil profiles in grasslands
Article Snippet: Understanding the correlations between the turfgrass rhizosphere soil (RS) microbial community assembly and soil extracellular enzymes (EEs) across soil profiles can contribute to enhancing the soil C pool and improving urban grassland ecosystem services.. This study collected rhizosphere soil from non-native Poa pratensis (Poa L.) and native Carex leucochlora (Carex L.) at depths of 0–40 cm to investigate soil properties, EEs, and microbial communities.. Compared to Poa, Carex significantly increased RS soil properties (i.e., organic carbon (SOC), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and available NPK), C-, and Nacquisition (acq) enzymes, and soil ecosystem multifunction at 0–40 cm soil depths.

Article Title: Flavonoids of Mao Jian Green Tea Ameliorate Glycemic Metabolism in Type-2-Diabetic Rats via AMPK Signaling Pathways and Gut Microbiota Regulation.
Article Snippet: The hypervariable V3-V4 regions of bacterial 16S rRNA genes were amplified via PCR (ABI GeneAmp® 9700; Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) employing universal primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′- GGACTACHVGGGTWTCTAAT-3′). .. Amplicons were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc., Union City, CA, USA) and quantified with a QuantusTM Fluorometer (Promega (Beijing) Biotech Co., Ltd., Beijing, China). .. Sequencing libraries were constructed with the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific Corporation, Austin, TX, USA), and paired-end sequencing (2 × 300 bp) was performed on an Illumina MiSeq platform (Majorbio Bio-Pharm Technology Co., Shanghai, China).

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Article Title: The impact of rainfall changes on soil bacterial and fungal communities in Pinus yunnanensis
Article Snippet: PCR products from the same sample were mixed and recovered on a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), detected by electrophoresis on a 2% agarose gel, and quantified by QuantusTM Fluorometer (Promega, USA). .. The recovered products were purified by 2% agarose gel electrophoresis, detected and quantified by QuantusTM Fluorometer (Promega, USA). .. Library construction was performed using the VAHTS® Universal Plus DNA Library Prep Kit for MGI V2 (Vazyme Biotech Co., Ltd, China) on purified PCR products. (1) linker link; (2) Use magnetic beads to screen and remove adapter self-connecting fragments; (3) Enrichment of library templates using PCR amplification; (4) Magnetic bead recycling of PCR products; and (5) PCR product cyclization.

Article Title: Spatiotemporal characterization of the dynamic changes in the intestinal microbiota of Taihe Silky Fowl.
Article Snippet: As instructed by the manufacturer, the PCR products were extracted from a 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, CA, USA). .. The purified PCR products were subsequently quantified using a QuantusTM fluorometer (Promega, USA). .. After quantification, the purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina NextSeq 2000 platform (Illumina, San Diego, USA) according to the standard protocols of Majorbio Tech.

Gel Extraction:

Article Title: Insights into the interlinkages between rhizosphere soil extracellular enzymes and microbiome assemblages across soil profiles in grasslands
Article Snippet: Understanding the correlations between the turfgrass rhizosphere soil (RS) microbial community assembly and soil extracellular enzymes (EEs) across soil profiles can contribute to enhancing the soil C pool and improving urban grassland ecosystem services.. This study collected rhizosphere soil from non-native Poa pratensis (Poa L.) and native Carex leucochlora (Carex L.) at depths of 0–40 cm to investigate soil properties, EEs, and microbial communities.. Compared to Poa, Carex significantly increased RS soil properties (i.e., organic carbon (SOC), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and available NPK), C-, and Nacquisition (acq) enzymes, and soil ecosystem multifunction at 0–40 cm soil depths.

Article Title: Flavonoids of Mao Jian Green Tea Ameliorate Glycemic Metabolism in Type-2-Diabetic Rats via AMPK Signaling Pathways and Gut Microbiota Regulation.
Article Snippet: The hypervariable V3-V4 regions of bacterial 16S rRNA genes were amplified via PCR (ABI GeneAmp® 9700; Applied Biosystems, Thermo Fisher Scientific Inc., Waltham, MA, USA) employing universal primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′- GGACTACHVGGGTWTCTAAT-3′). .. Amplicons were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Scientific, Inc., Union City, CA, USA) and quantified with a QuantusTM Fluorometer (Promega (Beijing) Biotech Co., Ltd., Beijing, China). .. Sequencing libraries were constructed with the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific Corporation, Austin, TX, USA), and paired-end sequencing (2 × 300 bp) was performed on an Illumina MiSeq platform (Majorbio Bio-Pharm Technology Co., Shanghai, China).

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

other:

Article Title: An acidic polysaccharide from Lycium barbarum L: Isolation, purification, structural characterization, and therapeutic effects on ulcerative colitis.
Article Snippet: Addressing microbial imbalance is a critical therapeutic strategy for ulcerative colitis (UC).. Natural polysaccharides have attracted increasing attention for their potential in UC prevention.. In this study, we purified and characterized an acidic heteropolysaccharide (LBP 2–1) from Lycium barbarum L. and systematically evaluated its therapeutic effects on UC.

Article Title: Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR
Article Snippet: The concentration of the final DNA library was determined by Quantus Fluorometer (Promega) and ranged between 19 and 64 nM.

DNA Sequencing:

Article Title: Variations in salivary microbiota and metabolic phenotype related to oral lichen planus with psychiatric symptoms.
Article Snippet: The DNA was then amplified using primers 338 F and 806R targeting the 16S rRNA gene V3-V4 region on an ABI GeneAmp® 9700 PCR thermocycler (ABI, USA). .. PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified using a QuantusTM Fluorometer (Promega, USA), and used for library construction with the NEXTFLEX Rapid DNA-Seq Kit. .. Sequencing was conducted on the Illumina MiSeq PE300 platform (Illumina, USA), and raw data were processed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).

Amplification:

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Agarose Gel Electrophoresis:

Article Title: Faecalibacterium prausnitzii enhances intestinal IgA response by host-microbe derived inecalcitol in colitis.
Article Snippet: .. After PCR amplification, the product was extracted with 2% agarose gel, then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantusTM Fluorometer (Promega, USA). .. The amplicons were pooled in equal amounts and sequenced on MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA).

Article Title: The impact of rainfall changes on soil bacterial and fungal communities in Pinus yunnanensis
Article Snippet: PCR products from the same sample were mixed and recovered on a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), detected by electrophoresis on a 2% agarose gel, and quantified by QuantusTM Fluorometer (Promega, USA). .. The recovered products were purified by 2% agarose gel electrophoresis, detected and quantified by QuantusTM Fluorometer (Promega, USA). .. Library construction was performed using the VAHTS® Universal Plus DNA Library Prep Kit for MGI V2 (Vazyme Biotech Co., Ltd, China) on purified PCR products. (1) linker link; (2) Use magnetic beads to screen and remove adapter self-connecting fragments; (3) Enrichment of library templates using PCR amplification; (4) Magnetic bead recycling of PCR products; and (5) PCR product cyclization.



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The glycosylation of EphB2 is indispensable for ephrin-A5/B2 binding specificity (A) Top: western blot and Coomassie brilliant blue staining results of purified EphB2 protein before/after PNGase F treatment. Bottom: western blot results of endogenous EphB2 from adult and P10 mice hippocampus before/after PNGase F enzyme treatment. GAPDH served as a loading control. (B) Flow diagram of FCM assay. EphB2-expressing cells bound by pre-clustered ephrin-A5 or ephrin-B2 were recorded by flow cytometry and translated to the relative <t>MESF</t> values. (C) The FCM results are analyzed by the “one-site specific binding” model to fit the saturation binding curves and obtain the K D values. The K D of EphB2 WT to ephrin-B2 and ephrin-A5 are 3.526 nM and 111.9 nM; the K D of EphB2 4Q to ephrin-B2 and ephrin-A5 are 29.91 nM and 32.29 nM. (D and E) Representative confocal microscopy images of ligand-induced ligand/receptor colocalization assays upon stimulating with ephrin ligands. Green represents cells expressing EphB2 4Q, and red represents 647-labeled pre-clustered ephrin ligands. Scale bar: 10 μm. Averaged ratios of cells with EphB2 4Q/ephrins colocalization after stimulation are plotted as (E). Statistical significance was performed by two-way ANOVA; Tukey’s post-hoc multiple comparisons test; ns, not significant; ( n = 3). (F) Representative western blot results of immunoprecipitated C-terminal 3 × FLAG EphB2 WT or EphB2 4Q in HEK293T cells after ephrin-A5 or ephrin-B2 stimulation. (G) Measurement of relative EphB2 tyrosine phosphorylation, correlated with (F). Statistical significance was performed by one-way ANOVA; Tukey’s post-hoc multiple comparisons test, ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ( n = 3). All data are represented as mean ± SD.
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Low levels of CD123 are sufficient for SL-401-mediated cytotoxicity. (A) Correlation between CD123 levels and SL-401 induced cytotoxicity in patient AML cells. <t>CD123-MESF</t> on vehicle-treated AML samples (N=13) and viability after 48 hr of SL-401 (1 μg/ml) treatment were used for correlation analysis. (B) Changes in CD123 molecules in AML blasts after SL-401 treatment. CD123-MESF was determined on viable AML blasts after 24 hours of treatment with vehicle or SL-401 (N=13). Mean difference = 11086 (95% CI for mean difference: 2006, 20166), P =0.021. (C-D) CD123 expression and effect of SL-401 on umbilical cord blood derived CD34 + cells. (C) CD34 positive selected cells were used for multicolor flow cytometry analysis and colony formation assays. Colonies were counted 10–14 days after plating CD34 + cells with continuous presence of vehicle or SL-401(1 μg/ml) P =0.019. (D) Effect of SL-401 on umbilical cord blood liquid cultures. Non-enriched Cord blood samples (N=4 CB) were ficoll processed to obtain mononuclear cells and cultured in RPMI media with 20% FBS and GM-CSF, SCF and IL-3 (10 ng/ml) in presence of SL-401 and the cells were counted and immunophenotyped after 48 hours. Live CD34 + cell counts per ml of culture are shown in the graph. Vehicle vs . SL-401 10ng/ml not significant; Vehicle vs . SL-401 100ng/ml; P =0.0356 and Vehicle vs . SL-401 1 μg/ml; P =0.0089.
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Image Search Results


The glycosylation of EphB2 is indispensable for ephrin-A5/B2 binding specificity (A) Top: western blot and Coomassie brilliant blue staining results of purified EphB2 protein before/after PNGase F treatment. Bottom: western blot results of endogenous EphB2 from adult and P10 mice hippocampus before/after PNGase F enzyme treatment. GAPDH served as a loading control. (B) Flow diagram of FCM assay. EphB2-expressing cells bound by pre-clustered ephrin-A5 or ephrin-B2 were recorded by flow cytometry and translated to the relative MESF values. (C) The FCM results are analyzed by the “one-site specific binding” model to fit the saturation binding curves and obtain the K D values. The K D of EphB2 WT to ephrin-B2 and ephrin-A5 are 3.526 nM and 111.9 nM; the K D of EphB2 4Q to ephrin-B2 and ephrin-A5 are 29.91 nM and 32.29 nM. (D and E) Representative confocal microscopy images of ligand-induced ligand/receptor colocalization assays upon stimulating with ephrin ligands. Green represents cells expressing EphB2 4Q, and red represents 647-labeled pre-clustered ephrin ligands. Scale bar: 10 μm. Averaged ratios of cells with EphB2 4Q/ephrins colocalization after stimulation are plotted as (E). Statistical significance was performed by two-way ANOVA; Tukey’s post-hoc multiple comparisons test; ns, not significant; ( n = 3). (F) Representative western blot results of immunoprecipitated C-terminal 3 × FLAG EphB2 WT or EphB2 4Q in HEK293T cells after ephrin-A5 or ephrin-B2 stimulation. (G) Measurement of relative EphB2 tyrosine phosphorylation, correlated with (F). Statistical significance was performed by one-way ANOVA; Tukey’s post-hoc multiple comparisons test, ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ( n = 3). All data are represented as mean ± SD.

Journal: iScience

Article Title: Ligand preference of EphB2 receptor is selectively regulated by N-glycosylation

doi: 10.1016/j.isci.2025.112386

Figure Lengend Snippet: The glycosylation of EphB2 is indispensable for ephrin-A5/B2 binding specificity (A) Top: western blot and Coomassie brilliant blue staining results of purified EphB2 protein before/after PNGase F treatment. Bottom: western blot results of endogenous EphB2 from adult and P10 mice hippocampus before/after PNGase F enzyme treatment. GAPDH served as a loading control. (B) Flow diagram of FCM assay. EphB2-expressing cells bound by pre-clustered ephrin-A5 or ephrin-B2 were recorded by flow cytometry and translated to the relative MESF values. (C) The FCM results are analyzed by the “one-site specific binding” model to fit the saturation binding curves and obtain the K D values. The K D of EphB2 WT to ephrin-B2 and ephrin-A5 are 3.526 nM and 111.9 nM; the K D of EphB2 4Q to ephrin-B2 and ephrin-A5 are 29.91 nM and 32.29 nM. (D and E) Representative confocal microscopy images of ligand-induced ligand/receptor colocalization assays upon stimulating with ephrin ligands. Green represents cells expressing EphB2 4Q, and red represents 647-labeled pre-clustered ephrin ligands. Scale bar: 10 μm. Averaged ratios of cells with EphB2 4Q/ephrins colocalization after stimulation are plotted as (E). Statistical significance was performed by two-way ANOVA; Tukey’s post-hoc multiple comparisons test; ns, not significant; ( n = 3). (F) Representative western blot results of immunoprecipitated C-terminal 3 × FLAG EphB2 WT or EphB2 4Q in HEK293T cells after ephrin-A5 or ephrin-B2 stimulation. (G) Measurement of relative EphB2 tyrosine phosphorylation, correlated with (F). Statistical significance was performed by one-way ANOVA; Tukey’s post-hoc multiple comparisons test, ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ( n = 3). All data are represented as mean ± SD.

Article Snippet: Quantumtm MESF microsphere kits , Bangs Laboratories , PSD818.

Techniques: Glycoproteomics, Binding Assay, Western Blot, Staining, Purification, Control, Expressing, Flow Cytometry, Confocal Microscopy, Labeling, Immunoprecipitation, Phospho-proteomics

Low levels of CD123 are sufficient for SL-401-mediated cytotoxicity. (A) Correlation between CD123 levels and SL-401 induced cytotoxicity in patient AML cells. CD123-MESF on vehicle-treated AML samples (N=13) and viability after 48 hr of SL-401 (1 μg/ml) treatment were used for correlation analysis. (B) Changes in CD123 molecules in AML blasts after SL-401 treatment. CD123-MESF was determined on viable AML blasts after 24 hours of treatment with vehicle or SL-401 (N=13). Mean difference = 11086 (95% CI for mean difference: 2006, 20166), P =0.021. (C-D) CD123 expression and effect of SL-401 on umbilical cord blood derived CD34 + cells. (C) CD34 positive selected cells were used for multicolor flow cytometry analysis and colony formation assays. Colonies were counted 10–14 days after plating CD34 + cells with continuous presence of vehicle or SL-401(1 μg/ml) P =0.019. (D) Effect of SL-401 on umbilical cord blood liquid cultures. Non-enriched Cord blood samples (N=4 CB) were ficoll processed to obtain mononuclear cells and cultured in RPMI media with 20% FBS and GM-CSF, SCF and IL-3 (10 ng/ml) in presence of SL-401 and the cells were counted and immunophenotyped after 48 hours. Live CD34 + cell counts per ml of culture are shown in the graph. Vehicle vs . SL-401 10ng/ml not significant; Vehicle vs . SL-401 100ng/ml; P =0.0356 and Vehicle vs . SL-401 1 μg/ml; P =0.0089.

Journal: Haematologica

Article Title: The interleukin-3 receptor CD123 targeted SL-401 mediates potent cytotoxic activity against CD34 + CD123 + cells from acute myeloid leukemia/myelodysplastic syndrome patients and healthy donors

doi: 10.3324/haematol.2018.188193

Figure Lengend Snippet: Low levels of CD123 are sufficient for SL-401-mediated cytotoxicity. (A) Correlation between CD123 levels and SL-401 induced cytotoxicity in patient AML cells. CD123-MESF on vehicle-treated AML samples (N=13) and viability after 48 hr of SL-401 (1 μg/ml) treatment were used for correlation analysis. (B) Changes in CD123 molecules in AML blasts after SL-401 treatment. CD123-MESF was determined on viable AML blasts after 24 hours of treatment with vehicle or SL-401 (N=13). Mean difference = 11086 (95% CI for mean difference: 2006, 20166), P =0.021. (C-D) CD123 expression and effect of SL-401 on umbilical cord blood derived CD34 + cells. (C) CD34 positive selected cells were used for multicolor flow cytometry analysis and colony formation assays. Colonies were counted 10–14 days after plating CD34 + cells with continuous presence of vehicle or SL-401(1 μg/ml) P =0.019. (D) Effect of SL-401 on umbilical cord blood liquid cultures. Non-enriched Cord blood samples (N=4 CB) were ficoll processed to obtain mononuclear cells and cultured in RPMI media with 20% FBS and GM-CSF, SCF and IL-3 (10 ng/ml) in presence of SL-401 and the cells were counted and immunophenotyped after 48 hours. Live CD34 + cell counts per ml of culture are shown in the graph. Vehicle vs . SL-401 10ng/ml not significant; Vehicle vs . SL-401 100ng/ml; P =0.0356 and Vehicle vs . SL-401 1 μg/ml; P =0.0089.

Article Snippet: CD123 Molecules of Equivalent Soluble Fluorochrome (MESF) on AML cells was calculated using QuantumTM MESF microsphere kit (Bangs Laboratories, Fishers, IN) and CD123 antibody (Clone: SSDCLY107D2; Beckman Coulter) after establishing calibration curve for each experiment per the manufacturer’s instructions.

Techniques: Expressing, Derivative Assay, Flow Cytometry, Cell Culture