quantitative methylation detection (agena bioscience)

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agena bioscience quantitative methylation detection

Alcohol + CI administration aggravated pancreatic histopathological damage and reduced HIF-1α <t>methylation</t> in alcoholic AP rats. A HE staining was applied to assess pancreatic pathology injuries at various dosages of CI in rats (magnification 200×, scale bar: 100 μm), n = 3. B ELISA assay was performed for serum expression of HIF-1α and VEGF, n = 6. ▲ P < 0.05 and ▲▲ P < 0.01 vs. Control group; ★ P < 0.05 and ★★ P < 0.01 vs. Alcohol group. Note: AP: Acute Pancreatitis; CI: Cerulein; HE: Hematoxylin and Eosin; ELISA: Enzyme-Linked Immunosorbent Assay; HIF-1α: Hypoxia-inducible factor 1 alpha; VEGF: Vascular Endothelial Growth Factor

Quantitative Methylation Detection, supplied by agena bioscience, used in various techniques. Bioz Stars score: 96/100, based on 2219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+sequenom+massarray+assay/Methylation/pmc12465335-160-20-33
Average 96 stars, based on 2219 article reviews

quantitative methylation detection - by Bioz Stars, 2026-11

96/100 stars

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1) Product Images from "In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics"

Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics

Journal: Journal of Inflammation (London, England)

doi: 10.1186/s12950-025-00463-9

Alcohol + CI administration aggravated pancreatic histopathological damage and reduced HIF-1α methylation in alcoholic AP rats. A HE staining was applied to assess pancreatic pathology injuries at various dosages of CI in rats (magnification 200×, scale bar: 100 μm), n = 3. B ELISA assay was performed for serum expression of HIF-1α and VEGF, n = 6. ▲ P < 0.05 and ▲▲ P < 0.01 vs. Control group; ★ P < 0.05 and ★★ P < 0.01 vs. Alcohol group. Note: AP: Acute Pancreatitis; CI: Cerulein; HE: Hematoxylin and Eosin; ELISA: Enzyme-Linked Immunosorbent Assay; HIF-1α: Hypoxia-inducible factor 1 alpha; VEGF: Vascular Endothelial Growth Factor

Figure Legend Snippet: Alcohol + CI administration aggravated pancreatic histopathological damage and reduced HIF-1α methylation in alcoholic AP rats. A HE staining was applied to assess pancreatic pathology injuries at various dosages of CI in rats (magnification 200×, scale bar: 100 μm), n = 3. B ELISA assay was performed for serum expression of HIF-1α and VEGF, n = 6. ▲ P < 0.05 and ▲▲ P < 0.01 vs. Control group; ★ P < 0.05 and ★★ P < 0.01 vs. Alcohol group. Note: AP: Acute Pancreatitis; CI: Cerulein; HE: Hematoxylin and Eosin; ELISA: Enzyme-Linked Immunosorbent Assay; HIF-1α: Hypoxia-inducible factor 1 alpha; VEGF: Vascular Endothelial Growth Factor

Techniques Used: Methylation, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Control

Alcohol + CI exacerbated hepatic and pancreatic injury and inflammatory response through HIF-1α methylation in alcoholic AP rats. A , B The serum content of lipase and amylase was examined by ELISA assay, n = 6; C - H Biochemical analysis was performed to measure the serum levels of AST, ALT, GGT, BUN, ALP, and CREA, n = 6; I Western blot analysis for TNF-α, IL-6, and IL-1β expression in pancreas of rats was made, n = 3; J MSP was used to evaluate HIF-1α methylation in pancreas of rats, n = 3; K Western blot analysis was employed to assess the expression of mTOR, p-mTOR, HIF-1α, and VEGF in pancreas of rats, n = 3. ▲ P < 0.05 and ▲▲ P < 0.01 vs. Control group; ★ P < 0.05 and ★★ P < 0.01 vs. Alcohol group. Note: AP: Acute Pancreatitis; CI: Cerulein; AST: Aspartate Aminotransferase; ALT: Alanine Aminotransferase; GGT: Gamma-Glutamyl Transferase; BUN: Blood Urea Nitrogen; ALP: Alkaline Phosphatase; CREA: Creatinine; TNF-α: Tumor Necrosis Factor-alpha; IL-6: Interleukin 6; IL-1β: Interleukin 1 beta; MSP: Methylation-Specific PCR (Polymerase Chain Reaction); mTOR: Mammalian Target of Rapamycin; p-Mtor: Phosphorylated Mammalian Target of Rapamycin; HIF-1α: Hypoxia-Inducible Factor 1 Alpha, VEGF: Vascular Endothelial Growth Factor

Figure Legend Snippet: Alcohol + CI exacerbated hepatic and pancreatic injury and inflammatory response through HIF-1α methylation in alcoholic AP rats. A , B The serum content of lipase and amylase was examined by ELISA assay, n = 6; C - H Biochemical analysis was performed to measure the serum levels of AST, ALT, GGT, BUN, ALP, and CREA, n = 6; I Western blot analysis for TNF-α, IL-6, and IL-1β expression in pancreas of rats was made, n = 3; J MSP was used to evaluate HIF-1α methylation in pancreas of rats, n = 3; K Western blot analysis was employed to assess the expression of mTOR, p-mTOR, HIF-1α, and VEGF in pancreas of rats, n = 3. ▲ P < 0.05 and ▲▲ P < 0.01 vs. Control group; ★ P < 0.05 and ★★ P < 0.01 vs. Alcohol group. Note: AP: Acute Pancreatitis; CI: Cerulein; AST: Aspartate Aminotransferase; ALT: Alanine Aminotransferase; GGT: Gamma-Glutamyl Transferase; BUN: Blood Urea Nitrogen; ALP: Alkaline Phosphatase; CREA: Creatinine; TNF-α: Tumor Necrosis Factor-alpha; IL-6: Interleukin 6; IL-1β: Interleukin 1 beta; MSP: Methylation-Specific PCR (Polymerase Chain Reaction); mTOR: Mammalian Target of Rapamycin; p-Mtor: Phosphorylated Mammalian Target of Rapamycin; HIF-1α: Hypoxia-Inducible Factor 1 Alpha, VEGF: Vascular Endothelial Growth Factor

Techniques Used: Methylation, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control, Polymerase Chain Reaction

Polymerase Chain Reaction:

Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions.
Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [82,83]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [81,84]; (iii) bisulfite sequencing PCR [76]; (iv) bisulfite pyrosequencing [85–87]; (v) droplet digital PCR (ddPCR) [88–90]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [91]. ..

Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions
Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [ , ]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [ , ]; (iii) bisulfite sequencing PCR [ ]; (iv) bisulfite pyrosequencing [ , , ]; (v) droplet digital PCR (ddPCR) [ , , ]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [ ]. ..

Methylation:

Article Title: Differential miRNA expression during replicative senescence of dental pulp stem cells with potential for forensic age assessment.
Article Snippet: .. Freire-Aradas A, Phillips C, Mosquera-Miguel A, Girón-Santamaría L, Gómez-Tato A, Casares De Cal M, et al. Development of a methylation marker set for forensic age estimation using analysis of public methylation data and the Agena Bioscience EpiTYPER system. ..

Methylation Sequencing:

Digital PCR:

Marker:

other:

Article Title: Chronic heart failure and GPX3 promoter methylation: A clinical-epigenetic analysis.
Article Snippet: Background: Selenoprotein GPX3 is linked to Chronic Heart Failure (CHF), but its promoter methylation patterns in CHF remain unclear.. Objective: To explore CpG methylation in the GPX3 promoter region and its association with clinical parameters in CHF.. Methods: Twenty CHF patients and twenty healthy controls were included.

DNA Methylation Assay:

Article Title: ADAMTS9-AS2 acts as an epigenetic brake to constrain DNMT3B-mediated CADM2 silencing in esophageal squamous cell carcinoma metastasis
Article Snippet: .. Targeted DNA methylation validation was performed using the MassARRAY EpiTYPER platform (Agena Bioscience) on 24 paired ESCC tumor and adjacent normal tissues. .. Genomic DNA underwent bisulfite conversion (EZ DNA Methylation-Lightning Kit, Zymo Research), followed by PCR amplification of the CADM2 locus flanking the cg03455765 region.

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