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quantiblue assay reagent  (InvivoGen)


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    Structured Review

    InvivoGen quantiblue assay reagent
    Quantiblue Assay Reagent, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1988 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantiblue/QUANTI-Blue+Solution/pmc12916478-252-0-5
    Average 97 stars, based on 1988 article reviews
    quantiblue assay reagent - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNA.
    Article Snippet: The HEK-Blue hTLR9 reporter assay (InvivoGen, hkb-htlr9) was used to assess the immunogenicity of SONAR ssDNA according to the manufacturer’s instructions.

    Transferring:

    Article Title: Distinct pro-inflammatory responses to pristine and microbially contaminated PET nanoplastics in a human alveolar cell co-culture
    Article Snippet: Purified lipoteichoic acid (LTA) from S. aureus (#tlrl-psltam, Invivogen, France) and ultra-purified lipopolysaccharide (LPS) from E. coli (#tlrl-3pelps, Invivogen, France) at a final concentration of 100 ng/mL were used as positive controls for activation of TLR2 and TLR4, respectively. .. The cells were then left to incubate for another 24 h before transferring 20 μL of the supernatant to a new 96-well plate and adding 180 μL of QUANTI-BlueTM Solution (#rep-qbs, Invivogen, France) to each of the wells. .. Following a 3-h incubation at 37 °C, the color development was measured at an absorbance of 649 nm using a BioTek Synergy Neo2 multi-mode microplate reader with Gen four software (BioTek Instruments, USA).

    Sterility:

    Article Title: HIV tri-specific killer engagers directly enhance NK cell function and safely expand NK cells in vivo.
    Article Snippet: .. On day 2, QUANTI-blue Solution (InvivoGen, Cat# rep-qbs3) was prepared by mixing 100ul of QUANTIblue and 100 μl of QB buffer in 9.8 mL of sterile H2O. ..

    Incubation:

    Article Title: Acetylation and accessibility of Cryptococcus neoformans cell wall chitosans influence the strength of host immune responses
    Article Snippet: .. Following incubation, 20 μL of culture supernatant was collected to measure SEAP activity using Quanti-Blue (InvivoGen) as the substrate. ..

    Activity Assay:

    Article Title: Acetylation and accessibility of Cryptococcus neoformans cell wall chitosans influence the strength of host immune responses
    Article Snippet: .. Following incubation, 20 μL of culture supernatant was collected to measure SEAP activity using Quanti-Blue (InvivoGen) as the substrate. ..

    Article Title: Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization.
    Article Snippet: After 48 h, cells were stimulated with purified EVs at different doses (from 5 x 105 to 5 x 107 particles/mL for HEK-Blue hTLR2 and from 7.5 x 108 to 5.0 x 1010 particles/mL for HEK-Blue hTLR7) for 24 h. 10 ng/mL of FSL-1 (tlrl-fsl, INVIVOGEN) or 100 ng/mL of R848 (tlrl-r848-1, INVIVOGEN) treated cells were used as a positive control for HEK-Blue hTLR2 and HEK-Blue hTLR7 respectively. .. Cell culture supernatants were assayed for SEAP activity by incubating 20 μL of supernatant with 180 μL of QUANTI-Blue solution (INVIVOGEN) at 37°C, according to the manufacturer’s instructions. .. SEAP activity was measured at 630 nm with the AGILENT BioTek Synergy H1 microplate reader.

    Cell Culture:

    Article Title: Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization.
    Article Snippet: After 48 h, cells were stimulated with purified EVs at different doses (from 5 x 105 to 5 x 107 particles/mL for HEK-Blue hTLR2 and from 7.5 x 108 to 5.0 x 1010 particles/mL for HEK-Blue hTLR7) for 24 h. 10 ng/mL of FSL-1 (tlrl-fsl, INVIVOGEN) or 100 ng/mL of R848 (tlrl-r848-1, INVIVOGEN) treated cells were used as a positive control for HEK-Blue hTLR2 and HEK-Blue hTLR7 respectively. .. Cell culture supernatants were assayed for SEAP activity by incubating 20 μL of supernatant with 180 μL of QUANTI-Blue solution (INVIVOGEN) at 37°C, according to the manufacturer’s instructions. .. SEAP activity was measured at 630 nm with the AGILENT BioTek Synergy H1 microplate reader.



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