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Avantor qscript flex cdna kit
Qscript Flex Cdna Kit, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qscript+flex+cdna+kit/qscript+flex+cdna+kit/us08889356-1253-7-11
Average 90 stars, based on 1 article reviews
qscript flex cdna kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: DNA targeting specificity of RNA-guided Cas9 nucleases
Article Snippet: The maximum upper bounds and minimum lower bounds for each biological replicate are listed in Supplementary Tables 5–8 . qRT-PCR analysis of relative Cas9 and sgRNA expression 72 h post-transfection, total RNA from 293FT cells was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers ( Supplementary Table 2 ). qPCR analysis was done with Fast SYBR Green Master Mix (Life Technologies) and custom primers ( Supplementary Table 2 ), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

SYBR Green Assay:

Article Title: DNA targeting specificity of RNA-guided Cas9 nucleases
Article Snippet: The maximum upper bounds and minimum lower bounds for each biological replicate are listed in Supplementary Tables 5–8 . qRT-PCR analysis of relative Cas9 and sgRNA expression 72 h post-transfection, total RNA from 293FT cells was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers ( Supplementary Table 2 ). qPCR analysis was done with Fast SYBR Green Master Mix (Life Technologies) and custom primers ( Supplementary Table 2 ), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Control:

Article Title: DNA targeting specificity of RNA-guided Cas9 nucleases
Article Snippet: The maximum upper bounds and minimum lower bounds for each biological replicate are listed in Supplementary Tables 5–8 . qRT-PCR analysis of relative Cas9 and sgRNA expression 72 h post-transfection, total RNA from 293FT cells was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers ( Supplementary Table 2 ). qPCR analysis was done with Fast SYBR Green Master Mix (Life Technologies) and custom primers ( Supplementary Table 2 ), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

Article Title: CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen). .. Reverse-strand synthesis for sgRNAs was performed with qScript Flex cDNA kit (VWR) and custom first-strand synthesis primers (Tables J and K). qPCR analysis was performed with Fast SYBR Green Master Mix (Life Technologies) and custom primers (Tables J and K), using GAPDH as an endogenous control. ..

other:

Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
Article Snippet: 72 hours post-transfection, total RNA was harvested with miRNeasy Micro Kit (Qiagen).



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RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 <t>cDNA,</t> transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).
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Image Search Results


RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 cDNA, transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

Journal: eLife

Article Title: EPB41L4A-AS1 long noncoding RNA acts in both cis - and trans -acting transcriptional regulation and controls nucleolar biology

doi: 10.7554/eLife.106846

Figure Lengend Snippet: RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 cDNA, transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

Article Snippet: RNA was then retrotranscribed using the qScript Flex cDNA Synthesis Kit (Quanta Bio), following standard conditions as suggested by the manufacturer.

Techniques: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, Control, Standard Deviation