qscript flex cdna synthesis kit (Quanta Biosciences)

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Quanta Biosciences qscript flex cdna synthesis kit

RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 <t>cDNA,</t> transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

Qscript Flex Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qscript+flex+cdna+kit/qScript+Flex+cDNA+Synthesis+Kit/pmc12975130-260-6-11
Average 96 stars, based on 293 article reviews

qscript flex cdna synthesis kit - by Bioz Stars, 2026-11

96/100 stars

Images

1) Product Images from "EPB41L4A-AS1 long noncoding RNA acts in both cis - and trans -acting transcriptional regulation and controls nucleolar biology"

Article Title: EPB41L4A-AS1 long noncoding RNA acts in both cis - and trans -acting transcriptional regulation and controls nucleolar biology

Journal: eLife

doi: 10.7554/eLife.106846

Figure Legend Snippet: RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 cDNA, transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

Techniques Used: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, Control, Standard Deviation

Generated:

Article Title: Cold activates a BH2 metabolic network promoting longevity and proteostasis.
Article Snippet: qPCR Total RNA was isolated from synchronized day-5 adult worms using RNAbee (Tel-Test, CS501-B). .. We then generated cDNA from isolated RNA using the qScript Flex cDNA synthesis kit (Quantabio). .. Quantitative PCR (qPCR) assays were performed with SYBR Green and a 1:20 dilution of cDNA on the CFX384 Real-Time System (Bio-Rad).

Article Title: SIR-2.3/SIRT4 loss enhances proteostasis and neuronal resilience via AMPK-induced autophagy in Huntington’s disease models
Article Snippet: Total RNA of human cell cultures was extracted using RNAbee (Tel-Test Inc.). .. Complementary DNA (cDNA) was generated using qScript Flex cDNA synthesis kit (Quantabio). .. SybrGreen real-time quantitative PCR (qPCR) experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad) following the manufacturer’s instructions.

Article Title: Aging alters synergistic microRNA networks in exosomes to stimulate repair in bleomycin-induced lung injury and skin wound healing
Article Snippet: The following 451 Jo urn al Pr e-p roo f 22 day, the slides were rinsed and incubated for 30 min with MACH2 goat anti-rabbit IgG-452 HRP polymer conjugate (Cat. RHRP520, Biocare, Concord, CA), followed by the TSA 453 fluorophore VIVID 650 (Cat. 7527, Bio-Techne, Minneapolis, MN; 1:1,000) for 15 min. 454 Slides were mounted in Vectashield VIBRANCE Plus DAPI (Cat. H-1800, Vector Labs). .. For microRNA analyses, cDNA was 461 generated using qScriptTM microDNA cDNA Synthesis Kit (Quanta Biosciences, Beverly, 462 MA) according to manufacturer’s instructions (23). .. Amplification of all microRNAs was 463 performed using specific primers, let-7b, miR-10a-5p, miR-29a-5p, miR-34a-5p, miR-464 125a-5p, miR-146a-5p and miR-181a-5p (IDT, Coralville, IA) using Real-Time SYBR 465 Green qRT-PCR Amplification kit (Quanta Biosciences, Beverly, MA).

Isolation:

cDNA Synthesis:

Article Title: Identifying intra-hospital Norovirus GII transmission using whole-genome sequencing.
Article Snippet: Whole-genome amplification and next-generation sequencing Primers were synthesized by Integrated DNA Technologies (Coralville, Iowa, USA) using standard desalting. .. QuantaBio qScript Ultra Flex Kit was used to perform first strand cDNA synthesis with 4 μL of 5X qScript Ultra Reaction Mix, 2 μL 10X GSP Enhancer, 2 μL Tx30SXN (1μM), 7 μl nuclease free water, and 5 μL of viral RNA per reaction. .. The samples were processed in a Verity 96-well thermal cycler (ThermoFisher Scientific, Waltham, Massachusetts, USA) at 55°C for 10 minutes and then 85°C for 5 minutes and infinite hold at 4°C.

Article Title: EPB41L4A-AS1 long noncoding RNA acts in both cis - and trans -acting transcriptional regulation and controls nucleolar biology
Article Snippet: The isolated RNA was then resuspended in DNase/RNase-free H 2 O and DNase-treated using the Baseline-ZERO DNase (Biosearch Technologies), following the manufacturer’s recommendations and incubating the RNAs for 1 hr at 37°C. .. RNA was then retrotranscribed using the qScript Flex cDNA Synthesis Kit (Quanta Bio), following standard conditions as suggested by the manufacturer. .. The resulting cDNA was finally diluted 1:5-1:10 with DNase/RNase-free H 2 O and used as input for Quantitative Real-Time PCR using the Fast SYBR Green Master Mix (Applied Biosystems).

Article Title: Behavioral and epileptic phenotypes in a CHD2-related developmental delay model.
Article Snippet: 1Department of Human Molecular Genetics and Biochemistry, Gray Faculty of Medical & Health Sciences, Goldschleger Eye Research Institute, Tel Aviv University, Tel Aviv, Israel 2Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel 3Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel 4Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel

Synthesized:

Article Title: AGS compounds as a novel therapeutic approach in a mouse model of Alzheimer’s disease
Article Snippet: .. RNA was extracted using Bio Tri RNA (Bio-Labs) according to the manufacturer’s instructions, and cDNA was synthesized with the qScript cDNA kit (QuantaBio). ..

Reverse Transcription:

Article Title: Effect of LPS and IFN-γ transient inflammatory priming on periodontal ligament cells and Schwann cells
Article Snippet: Total mRNA was extracted using the PureLink RNA kit (Thermo Fisher Scientific, Waltham, MA, USA) following the TRIzol protocol. .. Subsequently, 0.37 μg of total RNA was reverse transcribed using the qScript cDNA kit (Quantabio, Beverly, USA). .. The obtained cDNA was used for qPCR with SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, USA).

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