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BIOTAGE pyrosequencer id
Pyrosequencer Id, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing/pyrosequencer+id/pmc05339435-68-4-14
Average 90 stars, based on 1 article reviews
pyrosequencer id - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

DNA Methylation Assay:

Article Title: Frequency and characterization of DNA methylation defects in children born SGA
Article Snippet: Single-strands were prepared using the Vacuum Prep Tool (Biotage, Uppsala, Sweden), followed by a denaturation step at 85 °C for 2 min and final sequencing primer hybridization. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to quantify the ratio T:C (mC:C) at the analyzed CpG sites. .. Assays were validated using a commercial in vitro methylated DNA as positive control (Millipore, Hilden, Germany) and pooled DNA from 20 healthy donors (10 males, 10 females).

Article Title: Chromosomal imbalances and partial uniparental disomies in primary central nervous system lymphoma.
Article Snippet: Single strands were prepared using the VacuumPrep Tool (Biotage, Uppsala, Sweden), followed by a denaturation step at 85 1C for 2 min and final sequencing primer (50-CCCTCTACCCACCTAAAT-30) hybridization. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to quantify the ratio T:C (mC:C) at the CpG sites analyzed. .. Assays were validated using an in vitro methylated DNA (Millipore, Billerica, MA, USA) and pooled DNA was isolated from 20 peripheral blood samples from 10 men and 10 women.

Article Title: Genomic loss of the putative tumor suppressor gene E2A in human lymphoma
Article Snippet: For E2A amplification reactions, PyroMark PCR kit (QIAGEN) was used according to standard protocols. .. After initial denaturation (95°C for 15 min), PCR consisted of 45 cycles of 94°C for 30 s, annealing temperature for 30 s, and 72°C for 30 s, followed by a final synthesis at 72°C for 10 min. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to evaluate the ratio T:C (mC:C) at the CpG sites analyzed. .. All assays were optimized and validated using commercially available completely methylated DNA (Millipore) and pooled DNA isolated from peripheral blood of 10 healthy male and female controls, respectively.

Article Title: The tissue is the issue: improved methylome analysis from paraffin-embedded tissues by application of the HOPE technique.
Article Snippet: PCR products were verified by gel electrophoresis and single strands were prepared using the Vacuum Prep Tool (Biotage, Uppsala, Sweden). .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage). ..

Article Title: Polynucleotides for medical use
Article Snippet: Using the VacuumPrep Tool (Biotage) single strands were prepared followed by a denaturation step at 85° C. for two min and final sequencing primer hybridization. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to evaluate the ratio T:C (mC:C) at the CpG sites analyzed. .. All assays were optimized and validated using commercially available completely methylated DNA (Millipore) and pooled DNA isolated from peripheral blood of 10 healthy male and female controls, respectively.

Article Title: Androgen receptor mutations are associated with altered epigenomic programming as evidenced by HOXA5 methylation.
Article Snippet: Single strands were prepared using the Vacuum Prep Tool (Biotage, Uppsala, Sweden) followed by a denaturation step at 85 ° C for 2 min and final sequencing primer (5 -GGT TTT GAA AGT TGT G-3 ) hybridisation. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to quantify the ratio T:C (mC:C) at the analyzed CpG sites. .. Assays were validated using a commercial in vitro methylated DNA as positive control and whole genome amplification DNA as negative control (GE Healthcare, Munich, Germany).

Software:

Article Title: Frequency and characterization of DNA methylation defects in children born SGA
Article Snippet: Single-strands were prepared using the Vacuum Prep Tool (Biotage, Uppsala, Sweden), followed by a denaturation step at 85 °C for 2 min and final sequencing primer hybridization. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to quantify the ratio T:C (mC:C) at the analyzed CpG sites. .. Assays were validated using a commercial in vitro methylated DNA as positive control (Millipore, Hilden, Germany) and pooled DNA from 20 healthy donors (10 males, 10 females).

Article Title: Chromosomal imbalances and partial uniparental disomies in primary central nervous system lymphoma.
Article Snippet: Single strands were prepared using the VacuumPrep Tool (Biotage, Uppsala, Sweden), followed by a denaturation step at 85 1C for 2 min and final sequencing primer (50-CCCTCTACCCACCTAAAT-30) hybridization. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to quantify the ratio T:C (mC:C) at the CpG sites analyzed. .. Assays were validated using an in vitro methylated DNA (Millipore, Billerica, MA, USA) and pooled DNA was isolated from 20 peripheral blood samples from 10 men and 10 women.

Article Title: Genomic loss of the putative tumor suppressor gene E2A in human lymphoma
Article Snippet: For E2A amplification reactions, PyroMark PCR kit (QIAGEN) was used according to standard protocols. .. After initial denaturation (95°C for 15 min), PCR consisted of 45 cycles of 94°C for 30 s, annealing temperature for 30 s, and 72°C for 30 s, followed by a final synthesis at 72°C for 10 min. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to evaluate the ratio T:C (mC:C) at the CpG sites analyzed. .. All assays were optimized and validated using commercially available completely methylated DNA (Millipore) and pooled DNA isolated from peripheral blood of 10 healthy male and female controls, respectively.

Article Title: The tissue is the issue: improved methylome analysis from paraffin-embedded tissues by application of the HOPE technique.
Article Snippet: PCR products were verified by gel electrophoresis and single strands were prepared using the Vacuum Prep Tool (Biotage, Uppsala, Sweden). .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage). ..

Article Title: Polynucleotides for medical use
Article Snippet: Using the VacuumPrep Tool (Biotage) single strands were prepared followed by a denaturation step at 85° C. for two min and final sequencing primer hybridization. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to evaluate the ratio T:C (mC:C) at the CpG sites analyzed. .. All assays were optimized and validated using commercially available completely methylated DNA (Millipore) and pooled DNA isolated from peripheral blood of 10 healthy male and female controls, respectively.

Article Title: Androgen receptor mutations are associated with altered epigenomic programming as evidenced by HOXA5 methylation.
Article Snippet: Single strands were prepared using the Vacuum Prep Tool (Biotage, Uppsala, Sweden) followed by a denaturation step at 85 ° C for 2 min and final sequencing primer (5 -GGT TTT GAA AGT TGT G-3 ) hybridisation. .. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to quantify the ratio T:C (mC:C) at the analyzed CpG sites. .. Assays were validated using a commercial in vitro methylated DNA as positive control and whole genome amplification DNA as negative control (GE Healthcare, Munich, Germany).

Polymerase Chain Reaction:

Article Title: Genomic loss of the putative tumor suppressor gene E2A in human lymphoma
Article Snippet: For E2A amplification reactions, PyroMark PCR kit (QIAGEN) was used according to standard protocols. .. After initial denaturation (95°C for 15 min), PCR consisted of 45 cycles of 94°C for 30 s, annealing temperature for 30 s, and 72°C for 30 s, followed by a final synthesis at 72°C for 10 min. Pyrosequencing was performed using the Pyrosequencer ID and the DNA methylation analysis software Pyro Q-CpG 1.0.9 (Biotage), which was also used to evaluate the ratio T:C (mC:C) at the CpG sites analyzed. .. All assays were optimized and validated using commercially available completely methylated DNA (Millipore) and pooled DNA isolated from peripheral blood of 10 healthy male and female controls, respectively.



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