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BIOTAGE pyrosequencing vacuum prep tool
Pyrosequencing Vacuum Prep Tool, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing/pyrosequencing+vacuum+prep+tool/pm29185345-50-29-32
Average 90 stars, based on 1 article reviews
pyrosequencing vacuum prep tool - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures.
Article Snippet: .. Following this, the PCR product was made single stranded to act as a template in a pyrosequencing reaction by washing with ethanol 70% and denaturation buffer using a Pyrosequencing Vacuum Prep Tool (Biotage AB, Uppsala, Sweden). .. The pyrosequencing runs were performed to obtain a pyrogram from each PCR reaction, using software for analysis in allele-quantification mode, in a total volume of 40 μl per well, including 38.4 μl of annealing buffer and 1.6 μl of 10 pM sequencing primer with suspended beads containing the sample DNA.

Article Title: Mitochondrial aggregation caused by cytochalasin B compromises the efficiency and safety of three-parent embryo.
Article Snippet: .. Briefly, single-stranded biotinylated DNA was prepared from the PCR product with Pyrosequencing Vacuum Prep Tool (Biotage AB, Uppsala, Sweden). .. After mixing with 40 ll annealing buffer and 1.5 ll sequencing primer, samples were denaturated at 85 C for 2 min. Then pyrosequencing was performed on a PyroMark Q96 ID platform (Qiagen, Hilden, Germany).

Article Title: Welcome to the 10th volume of Epigenomics.
Article Snippet: .. Following this, the PCR product was made single stranded to act as a template in a pyrosequencing reaction by washing with ethanol 70% and denaturation buffer using a Pyrosequencing Vacuum Prep Tool (Biotage AB, Uppsala, Sweden). .. The pyrosequencing runs were performed to obtain a pyrogram from each PCR reaction, using software for analysis in allele-quantification mode, in a total volume of 40 μl per well, including 38.4 μl of annealing buffer and 1.6 μl of 10 pM sequencing primer with suspended beads containing the sample DNA.

Article Title: Matching Mitochondrial DNA Haplotypes for Circumventing Tissue-Specific Segregation Bias
Article Snippet: .. Single-stranded biotinylated PCR products were prepared for sequencing by Pyrosequencing Vacuum Prep Tool (Biotage AB) according the protocol of PyroMark Q96 ID platform (Qiagen). .. Primer used for pyrosequencing was 5’-GAATAAACCCAGAAGAGAGT-3’.

Article Title: Pharmacogenetic Approach to Toxicity in Breast Cancer Patients Treated with Taxanes.
Article Snippet: All PCR reactions were performed in a final volume of 50 μl containing 40 ng of genomic DNA, 10 pmol of each primer, 0.2 m dNTPs, the appropriate concentration of MgCl2 (Table I), PCR buffer and 1.5 U of Taq DNA polymerase (Takara Bio Inc. Otsu, Japan). .. Single-stranded DNA was isolated from the PCR reaction using the Pyrosequencing Vacuum Prep Workstation (Biotage) and Streptavidin SepharoseTM High Performance beads (Amersham Biosciences, Uppsala, Sweden) that bind to the biotinylated primer. ..

Article Title: Resveratrol Modulates SIRT1 and DNMT Functions and Restores LINE-1 Methylation Levels in ARPE-19 Cells under Oxidative Stress and Inflammation
Article Snippet: .. The biotinylated PCR product was purified and made single-stranded using the Pyrosequencing Vacuum PrepTool (Biotage, Inc., Charlottesville, VA, USA). .. The biotinylated single-stranded product was annealed to the pyrosequencing primer (5′-AGTTAGGTGTGGGATATAGT-3′) and then subjected to sequencing using an automatically generated nucleotide dispensation order.

Article Title: Epigenetic Inactivation of Notch-Hes Pathway in Human B-Cell Acute Lymphoblastic Leukemia
Article Snippet: The final biotin labeled PCR product was captured by Streptavidin Sepharose HP (Amersham Biosciences, Uppsala, Sweden). .. PCR products bound to the bead were purified and made single-stranded using a Pyrosequencing Vacuum Prep Tool (Biotage Inc, Uppsala,, Sweden). .. The sequencing primer was annealed to the single stranded PCR product and pyrosequencing was done using the PSQ HS 96 Pyrosequencing System (Biotage Inc, Uppsala, Sweden).

Purification:

Article Title: Impact of Chromosomal Rearrangement upon DNA Methylation Patterns in Leukemia
Article Snippet: In brief, 10 μL PCR products for each sequencing reaction were immobilized onto streptavidin-coated beads (Streptavidin Sepharose HP, GE Healthcare Biosciences, Pittsburgh, PA, USA) in binding buffer (10 mM Tris-HCl, pH 7.6, 2 M NaCl, 1 mM EDTA, 0.1% Tween 20) for 10 min. .. The biotinylated template was purified with the Pyrosequencing vacuum prep tool (Biotage, Uppsala, Sweden) and incubated with 10pmol/reaction individually with each sequencing primer in annealing buffer (20 mM Tris-acetate, pH 7.6 and 2 mM MgAc2). ..

Article Title: Resveratrol Modulates SIRT1 and DNMT Functions and Restores LINE-1 Methylation Levels in ARPE-19 Cells under Oxidative Stress and Inflammation
Article Snippet: .. The biotinylated PCR product was purified and made single-stranded using the Pyrosequencing Vacuum PrepTool (Biotage, Inc., Charlottesville, VA, USA). .. The biotinylated single-stranded product was annealed to the pyrosequencing primer (5′-AGTTAGGTGTGGGATATAGT-3′) and then subjected to sequencing using an automatically generated nucleotide dispensation order.

Article Title: Epigenetic Inactivation of Notch-Hes Pathway in Human B-Cell Acute Lymphoblastic Leukemia
Article Snippet: The final biotin labeled PCR product was captured by Streptavidin Sepharose HP (Amersham Biosciences, Uppsala, Sweden). .. PCR products bound to the bead were purified and made single-stranded using a Pyrosequencing Vacuum Prep Tool (Biotage Inc, Uppsala,, Sweden). .. The sequencing primer was annealed to the single stranded PCR product and pyrosequencing was done using the PSQ HS 96 Pyrosequencing System (Biotage Inc, Uppsala, Sweden).

Incubation:

Article Title: Impact of Chromosomal Rearrangement upon DNA Methylation Patterns in Leukemia
Article Snippet: In brief, 10 μL PCR products for each sequencing reaction were immobilized onto streptavidin-coated beads (Streptavidin Sepharose HP, GE Healthcare Biosciences, Pittsburgh, PA, USA) in binding buffer (10 mM Tris-HCl, pH 7.6, 2 M NaCl, 1 mM EDTA, 0.1% Tween 20) for 10 min. .. The biotinylated template was purified with the Pyrosequencing vacuum prep tool (Biotage, Uppsala, Sweden) and incubated with 10pmol/reaction individually with each sequencing primer in annealing buffer (20 mM Tris-acetate, pH 7.6 and 2 mM MgAc2). ..

Sequencing:

Article Title: Impact of Chromosomal Rearrangement upon DNA Methylation Patterns in Leukemia
Article Snippet: In brief, 10 μL PCR products for each sequencing reaction were immobilized onto streptavidin-coated beads (Streptavidin Sepharose HP, GE Healthcare Biosciences, Pittsburgh, PA, USA) in binding buffer (10 mM Tris-HCl, pH 7.6, 2 M NaCl, 1 mM EDTA, 0.1% Tween 20) for 10 min. .. The biotinylated template was purified with the Pyrosequencing vacuum prep tool (Biotage, Uppsala, Sweden) and incubated with 10pmol/reaction individually with each sequencing primer in annealing buffer (20 mM Tris-acetate, pH 7.6 and 2 mM MgAc2). ..

Article Title: Matching Mitochondrial DNA Haplotypes for Circumventing Tissue-Specific Segregation Bias
Article Snippet: .. Single-stranded biotinylated PCR products were prepared for sequencing by Pyrosequencing Vacuum Prep Tool (Biotage AB) according the protocol of PyroMark Q96 ID platform (Qiagen). .. Primer used for pyrosequencing was 5’-GAATAAACCCAGAAGAGAGT-3’.

Isolation:

Article Title: Pharmacogenetic Approach to Toxicity in Breast Cancer Patients Treated with Taxanes.
Article Snippet: All PCR reactions were performed in a final volume of 50 μl containing 40 ng of genomic DNA, 10 pmol of each primer, 0.2 m dNTPs, the appropriate concentration of MgCl2 (Table I), PCR buffer and 1.5 U of Taq DNA polymerase (Takara Bio Inc. Otsu, Japan). .. Single-stranded DNA was isolated from the PCR reaction using the Pyrosequencing Vacuum Prep Workstation (Biotage) and Streptavidin SepharoseTM High Performance beads (Amersham Biosciences, Uppsala, Sweden) that bind to the biotinylated primer. ..



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