Review




Structured Review

Pyrosequencing Inc pyrosequencing-based dna methylation analysis
(A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by <t>a</t> <t>pyrosequencing</t> based methylation assay of repetitive <t>DNA</t> elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).
Pyrosequencing Based Dna Methylation Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/dna+methylation+pyrosequencing/pmc03579934-135-1-0
Average 90 stars, based on 1 article reviews
pyrosequencing-based dna methylation analysis - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant"

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

Journal: PLoS ONE

doi: 10.1371/journal.pone.0056931

(A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).
Figure Legend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

Techniques Used: Methylation

Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.
Figure Legend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

Techniques Used: DNA Methylation Assay, Methylation

Related Articles

other:

Article Title: Hypomethylation of LINE-1 retrotransposons is associated with cadmium-induced testicular injury.
Article Snippet: Retrotransposons, as vital regulator of male fertility, are essential for spermatogenesis.. Cadmium (Cd) is an environmental toxicant and endocrine disruptor, targeting the reproductive system.. Growing evidence shows that Cd exposure can induce male infertility in mammals.

Article Title: Effects of PM Exposure on the Methylation of Clock Genes in a Population of Subjects with Overweight or Obesity.
Article Snippet: We adopted a repeated-measures design as DNA methylation measurements were run in duplicate for each subject, and the Pyrosequencing-based DNA methylation analysis tested a variable number of CpG positions according to CpG density in the promoter assay.

Article Title: Relationships between serotonin 1A receptor DNA methylation, self-reported history of childhood abuse and gray matter volume in major depression.
Article Snippet: Background: Early life adversity is a risk factor for psychopathology and is associated with epigenetic alterations in the 5-HT1A receptor gene promoter.. The 5-HT1A receptor mediates neurotrophic effects, which could affect brain structure and function.. We examined relationships between self-reported early childhood abuse, 5-HT1A receptor promoter DNA methylation, and gray matter volume (GMV) in Major Depressive Disorder (MDD).

Article Title: Epigenomic and Metabolomic Integration Reveals Dynamic Metabolic Regulation in Bladder Cancer
Article Snippet: DNA Methylation Pyrosequencing techniques and comparative analysis of large databases allow us to discover DNA methylation events, also important in tumor metabolism.

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant
Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

DNA Methylation Assay:

Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications
Article Snippet: .. Reading was performed for three independent experiments. ( b ) DNA methylation pyrosequencing confirming that treatment with 1 μM 5-azacytdine (AZA) impairs DNA methylation deposition at the reporter. ( c ) Fraction of cellular population that is in either a ‘off’, ‘low’ or ‘high’ expression state following epigenetic editing +/− AZA. ( d ) Dot plot showing log 10 single-cell expression ( N = 500 ) upon specific programming of H3K27me3, H2AK119ub or both polycomb modifications together relative to control. ..

Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications.
Article Snippet: .. Reading was performed for three independent experiments. (b) DNA methylation pyrosequencing confirming that treatment with 1 μM 5-azacytdine (AZA) impairs DNA methylation deposition at the reporter. (c) Fraction of cellular population that is in either a ‘off’, ‘low’ or ‘high’ expression state following epigenetic editing +/− AZA. (d) Dot plot showing log10 single-cell expression (N = 500) upon specific programming of H3K27me3, H2AK119ub or both polycomb modifications together relative to control. ..

Article Title: Physical Activity and Epigenetic Aging in Breast Cancer Treatment.
Article Snippet: .. Telomere length was measured using qPCR, and the ELOVL2-based epigenetic clock was assessed via DNA methylation pyrosequencing of the ELOVL2 promoter region. ..

Expressing:

Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications
Article Snippet: .. Reading was performed for three independent experiments. ( b ) DNA methylation pyrosequencing confirming that treatment with 1 μM 5-azacytdine (AZA) impairs DNA methylation deposition at the reporter. ( c ) Fraction of cellular population that is in either a ‘off’, ‘low’ or ‘high’ expression state following epigenetic editing +/− AZA. ( d ) Dot plot showing log 10 single-cell expression ( N = 500 ) upon specific programming of H3K27me3, H2AK119ub or both polycomb modifications together relative to control. ..

Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications.
Article Snippet: .. Reading was performed for three independent experiments. (b) DNA methylation pyrosequencing confirming that treatment with 1 μM 5-azacytdine (AZA) impairs DNA methylation deposition at the reporter. (c) Fraction of cellular population that is in either a ‘off’, ‘low’ or ‘high’ expression state following epigenetic editing +/− AZA. (d) Dot plot showing log10 single-cell expression (N = 500) upon specific programming of H3K27me3, H2AK119ub or both polycomb modifications together relative to control. ..

Single Cell:

Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications
Article Snippet: .. Reading was performed for three independent experiments. ( b ) DNA methylation pyrosequencing confirming that treatment with 1 μM 5-azacytdine (AZA) impairs DNA methylation deposition at the reporter. ( c ) Fraction of cellular population that is in either a ‘off’, ‘low’ or ‘high’ expression state following epigenetic editing +/− AZA. ( d ) Dot plot showing log 10 single-cell expression ( N = 500 ) upon specific programming of H3K27me3, H2AK119ub or both polycomb modifications together relative to control. ..

Control:

Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications
Article Snippet: .. Reading was performed for three independent experiments. ( b ) DNA methylation pyrosequencing confirming that treatment with 1 μM 5-azacytdine (AZA) impairs DNA methylation deposition at the reporter. ( c ) Fraction of cellular population that is in either a ‘off’, ‘low’ or ‘high’ expression state following epigenetic editing +/− AZA. ( d ) Dot plot showing log 10 single-cell expression ( N = 500 ) upon specific programming of H3K27me3, H2AK119ub or both polycomb modifications together relative to control. ..

Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications.
Article Snippet: .. Reading was performed for three independent experiments. (b) DNA methylation pyrosequencing confirming that treatment with 1 μM 5-azacytdine (AZA) impairs DNA methylation deposition at the reporter. (c) Fraction of cellular population that is in either a ‘off’, ‘low’ or ‘high’ expression state following epigenetic editing +/− AZA. (d) Dot plot showing log10 single-cell expression (N = 500) upon specific programming of H3K27me3, H2AK119ub or both polycomb modifications together relative to control. ..

Real-time Polymerase Chain Reaction:

Article Title: Physical Activity and Epigenetic Aging in Breast Cancer Treatment.
Article Snippet: .. Telomere length was measured using qPCR, and the ELOVL2-based epigenetic clock was assessed via DNA methylation pyrosequencing of the ELOVL2 promoter region. ..



Similar Products

86
Pyrosequencing Inc pyrosequencing based analysis
Pyrosequencing Based Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/16s+pyrosequencing+rrna/pm42128334-658-12-12
Average 86 stars, based on 1 article reviews
pyrosequencing based analysis - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

90
Pyrosequencing Inc pyrosequencing-based methylation analysis
Pyrosequencing Based Methylation Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/pyrosequencing+based+methylation+analysis/pm40647502-43-0-0
Average 90 stars, based on 1 article reviews
pyrosequencing-based methylation analysis - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Pyrosequencing Inc pyrosequencing-based bisulfite pcr analysis
Pyrosequencing Based Bisulfite Pcr Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/bisulfite+pyrosequencing/pm39414799-158-1-0
Average 90 stars, based on 1 article reviews
pyrosequencing-based bisulfite pcr analysis - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Pyrosequencing Inc pyrosequencing-based analysis
Pyrosequencing Based Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/pyrosequencingtm/pmc11485332-63-2-2
Average 90 stars, based on 1 article reviews
pyrosequencing-based analysis - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Pyrosequencing Inc pyrosequencing-based analysis of bacterial 16 s rrna (v3-v5 region)
Pyrosequencing Based Analysis Of Bacterial 16 S Rrna (V3 V5 Region), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/pyrosequencing+based+analysis+of+bacterial+16s+rrna++v3+v5+region+/pm37390712-146-22-16
Average 90 stars, based on 1 article reviews
pyrosequencing-based analysis of bacterial 16 s rrna (v3-v5 region) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Pyrosequencing Inc pyrosequencing-based analysis of bacterial 16s rdna
Pyrosequencing Based Analysis Of Bacterial 16s Rdna, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/pyrosequencing+based+analysis+of+bacterial+16s+rdna/pm36535571-175-18-12
Average 90 stars, based on 1 article reviews
pyrosequencing-based analysis of bacterial 16s rdna - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Pyrosequencing Inc pyrosequencing-based dna methylation analysis
(A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by <t>a</t> <t>pyrosequencing</t> based methylation assay of repetitive <t>DNA</t> elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).
Pyrosequencing Based Dna Methylation Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing-based+analysis/dna+methylation+pyrosequencing/pmc03579934-135-1-0
Average 90 stars, based on 1 article reviews
pyrosequencing-based dna methylation analysis - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


(A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: Methylation

Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: DNA Methylation Assay, Methylation