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pyrosequencing-based bisulfite pcr analysis  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc pyrosequencing-based bisulfite pcr analysis
    Pyrosequencing Based Bisulfite Pcr Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyrosequencing-based+analysis/bisulfite+pyrosequencing/pm39414799-158-1-0
    Average 90 stars, based on 1 article reviews
    pyrosequencing-based bisulfite pcr analysis - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Methylation:

    Article Title: Translational Fidelity Decline in the Aging Oocyte and Embryo Development
    Article Snippet: Duncan, et al. 2017 [ ] , Mouse follicles/oocytes , Aging , Proteostasis, nucleolus, ribosome metabolism , Single-follicle RNA-seq, nucleolar markers , Aging alters nucleolar organization and protein metabolism pathways in highly translational oocytes. .. Potabattula, et al. 2022 [ ] , Human + mouse GV oocytes , Aging , rDNA methylation , Single-oocyte bisulfite pyrosequencing , rDNA methylation increases with age; heterogeneity between oocytes suggests variable epigenetic aging. .. Reduced translational gatekeeping , CPEB1 network , Lower CPEB1 reduces repression and mistimes activation , Premature cell-cycle translation, altered meiotic timing.

    Article Title: A Bioinformatics and Wet-Lab-Based Pipeline Identifies CLDN10 and GJB2 as Epigenetically Silenced Tumor Suppressor Genes in Cutaneous Melanoma.
    Article Snippet: DNA from primary tissue was isolated by the NucleoSpin Tissue Kit (MACHEREY-NAGEL 740,952.250, Aachen, Germany). .. Promoter methylation was analyzed by combining bisulfite restriction analysis (CoBRA) and bisulfite pyrosequencing. .. For bisulfite treatment, we used the EZ DNA Methylation Kit (Zymo Research D5001, Zymo Research Europe GmbH, Freiburg, Germany).

    Article Title: A Bioinformatics and Wet-Lab-Based Pipeline Identifies CLDN10 and GJB2 as Epigenetically Silenced Tumor Suppressor Genes in Cutaneous Melanoma
    Article Snippet: DNA from primary tissue was isolated by the NucleoSpin Tissue Kit (MACHEREY-NAGEL 740,952.250, Aachen, Germany). .. Promoter methylation was analyzed by combining bisulfite restriction analysis (CoBRA) and bisulfite pyrosequencing. .. For bisulfite treatment, we used the EZ DNA Methylation Kit (Zymo Research D5001, Zymo Research Europe GmbH, Freiburg, Germany).

    Combined Bisulfite Restriction Analysis Assay:

    Article Title: A Bioinformatics and Wet-Lab-Based Pipeline Identifies CLDN10 and GJB2 as Epigenetically Silenced Tumor Suppressor Genes in Cutaneous Melanoma.
    Article Snippet: DNA from primary tissue was isolated by the NucleoSpin Tissue Kit (MACHEREY-NAGEL 740,952.250, Aachen, Germany). .. Promoter methylation was analyzed by combining bisulfite restriction analysis (CoBRA) and bisulfite pyrosequencing. .. For bisulfite treatment, we used the EZ DNA Methylation Kit (Zymo Research D5001, Zymo Research Europe GmbH, Freiburg, Germany).

    Article Title: A Bioinformatics and Wet-Lab-Based Pipeline Identifies CLDN10 and GJB2 as Epigenetically Silenced Tumor Suppressor Genes in Cutaneous Melanoma
    Article Snippet: DNA from primary tissue was isolated by the NucleoSpin Tissue Kit (MACHEREY-NAGEL 740,952.250, Aachen, Germany). .. Promoter methylation was analyzed by combining bisulfite restriction analysis (CoBRA) and bisulfite pyrosequencing. .. For bisulfite treatment, we used the EZ DNA Methylation Kit (Zymo Research D5001, Zymo Research Europe GmbH, Freiburg, Germany).

    other:

    Article Title: An epigenetic framework for understanding contextual influences on neural and socio-emotional development: Narrative review and case-study of the oxytocin receptor.
    Article Snippet: When assaying OXTRm using bisulfite pyrosequencing, variance across three technical replicates is often close to 2%.



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    (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by <t>a</t> <t>pyrosequencing</t> based methylation assay of repetitive <t>DNA</t> elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).
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    Image Search Results


    (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

    Journal: PLoS ONE

    Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

    doi: 10.1371/journal.pone.0056931

    Figure Lengend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

    Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

    Techniques: Methylation

    Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

    Journal: PLoS ONE

    Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

    doi: 10.1371/journal.pone.0056931

    Figure Lengend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

    Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

    Techniques: DNA Methylation Assay, Methylation