affinity purification (Miltenyi Biotec)
95
Structured Review
Miltenyi Biotec
affinity purification
Affinity Purification, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/purification+kit/%C2%B5MACS+GFP+Isolation+Kit/pm42393076-378-1-11
Average 95 stars, based on 123 article reviews
Affinity Purification, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/purification+kit/%C2%B5MACS+GFP+Isolation+Kit/pm42393076-378-1-11
Average 95 stars, based on 123 article reviews
affinity purification - by Bioz Stars,
2026-11
95/100 stars
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Isolation:Article Title: Autosomal recessive A20 zinc finger 7 mutation is associated with early-onset lupus-like disease Article Snippet: Transfected HEK293T cells were harvested and lysed with RIPA buffer (Thermo Fisher Scientific #89901) supplemented with protease and phosphatase inhibitor (Thermo Fisher Scientific #1861280). .. The overexpressed proteins were isolated using the μMACS Article Title: The OsMYB4 transcription factor enables coordinated improvement of grain yield and quality in rice. Article Snippet: .. The supernatants were subjected to immunoprecipitation using the Article Title: DDX39A unwinds viral RNA G-quadruplexes to limit α-Synuclein amyloidogenesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 3000 Invitrogen L3000-008 Lipofectamine 2000 Invitrogen 11668500 Lipofectamine RNAiMAX Invitrogen 13778100 4′,6-Diamidino-2-Phenylindole, Dilactate (DAPI) Molecular Probes D3571 Antifade Mounting Medium Vectashield H-1000 Trypsin recombinant, Proteomics Grade Roche 3708969001 ZipTip with 0.6 μL C18 resin Merck Millipore ZTC185960 Ni-NTA agarose Invitrogen R901-15 Poly-L-lysine hydrobromide Sigma-Aldrich P5899 L-Glutamine solution Sigma-Aldrich G7513 NeurobasalTM Plus Medium Gibco A3582901 B-27TM Plus Supplement (50×) Gibco A3582801 Thioflavin T Sigma-Aldrich T3516-5G NHS-Rhodamine Thermo Scientific 46406 Alexa Fluor 488 C5 Maleimide Thermo Scientific A10254 Critical commercial Incubation:Article Title: The autophagic and non-autophagic functions of the S. cerevisiae PROPPIN Hsv2. Article Snippet: Atg18, Atg21 and Hsv2 are homologous proteins that fulfill macroautophagic/autophagic and nonautophagic functions.. We now found that Atg21 interacts with Pep8/Vps26, Vps29 and Vps35, the components of the cargo selective complex of the retromer.. We identified Atg21 residues required for retromer binding and focused on two of them. Immunoprecipitation:Article Title: The OsMYB4 transcription factor enables coordinated improvement of grain yield and quality in rice. Article Snippet: .. The supernatants were subjected to immunoprecipitation using the Modification:Article Title: The OsMYB4 transcription factor enables coordinated improvement of grain yield and quality in rice. Article Snippet: .. The supernatants were subjected to immunoprecipitation using the other:Article Title: VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair. Article Snippet: The pLenti expression construct encoding Lyso-pHluorin (Addgene, 70113) was described in Rost et al.50. Recombinant:Article Title: DDX39A unwinds viral RNA G-quadruplexes to limit α-Synuclein amyloidogenesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 3000 Invitrogen L3000-008 Lipofectamine 2000 Invitrogen 11668500 Lipofectamine RNAiMAX Invitrogen 13778100 4′,6-Diamidino-2-Phenylindole, Dilactate (DAPI) Molecular Probes D3571 Antifade Mounting Medium Vectashield H-1000 Trypsin recombinant, Proteomics Grade Roche 3708969001 ZipTip with 0.6 μL C18 resin Merck Millipore ZTC185960 Ni-NTA agarose Invitrogen R901-15 Poly-L-lysine hydrobromide Sigma-Aldrich P5899 L-Glutamine solution Sigma-Aldrich G7513 NeurobasalTM Plus Medium Gibco A3582901 B-27TM Plus Supplement (50×) Gibco A3582801 Thioflavin T Sigma-Aldrich T3516-5G NHS-Rhodamine Thermo Scientific 46406 Alexa Fluor 488 C5 Maleimide Thermo Scientific A10254 Critical commercial Western Blot:Article Title: DDX39A unwinds viral RNA G-quadruplexes to limit α-Synuclein amyloidogenesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 3000 Invitrogen L3000-008 Lipofectamine 2000 Invitrogen 11668500 Lipofectamine RNAiMAX Invitrogen 13778100 4′,6-Diamidino-2-Phenylindole, Dilactate (DAPI) Molecular Probes D3571 Antifade Mounting Medium Vectashield H-1000 Trypsin recombinant, Proteomics Grade Roche 3708969001 ZipTip with 0.6 μL C18 resin Merck Millipore ZTC185960 Ni-NTA agarose Invitrogen R901-15 Poly-L-lysine hydrobromide Sigma-Aldrich P5899 L-Glutamine solution Sigma-Aldrich G7513 NeurobasalTM Plus Medium Gibco A3582901 B-27TM Plus Supplement (50×) Gibco A3582801 Thioflavin T Sigma-Aldrich T3516-5G NHS-Rhodamine Thermo Scientific 46406 Alexa Fluor 488 C5 Maleimide Thermo Scientific A10254 Critical commercial Liquid Chromatography:Article Title: DDX39A unwinds viral RNA G-quadruplexes to limit α-Synuclein amyloidogenesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 3000 Invitrogen L3000-008 Lipofectamine 2000 Invitrogen 11668500 Lipofectamine RNAiMAX Invitrogen 13778100 4′,6-Diamidino-2-Phenylindole, Dilactate (DAPI) Molecular Probes D3571 Antifade Mounting Medium Vectashield H-1000 Trypsin recombinant, Proteomics Grade Roche 3708969001 ZipTip with 0.6 μL C18 resin Merck Millipore ZTC185960 Ni-NTA agarose Invitrogen R901-15 Poly-L-lysine hydrobromide Sigma-Aldrich P5899 L-Glutamine solution Sigma-Aldrich G7513 NeurobasalTM Plus Medium Gibco A3582901 B-27TM Plus Supplement (50×) Gibco A3582801 Thioflavin T Sigma-Aldrich T3516-5G NHS-Rhodamine Thermo Scientific 46406 Alexa Fluor 488 C5 Maleimide Thermo Scientific A10254 Critical commercial Tissue:Article Title: DDX39A unwinds viral RNA G-quadruplexes to limit α-Synuclein amyloidogenesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 3000 Invitrogen L3000-008 Lipofectamine 2000 Invitrogen 11668500 Lipofectamine RNAiMAX Invitrogen 13778100 4′,6-Diamidino-2-Phenylindole, Dilactate (DAPI) Molecular Probes D3571 Antifade Mounting Medium Vectashield H-1000 Trypsin recombinant, Proteomics Grade Roche 3708969001 ZipTip with 0.6 μL C18 resin Merck Millipore ZTC185960 Ni-NTA agarose Invitrogen R901-15 Poly-L-lysine hydrobromide Sigma-Aldrich P5899 L-Glutamine solution Sigma-Aldrich G7513 NeurobasalTM Plus Medium Gibco A3582901 B-27TM Plus Supplement (50×) Gibco A3582801 Thioflavin T Sigma-Aldrich T3516-5G NHS-Rhodamine Thermo Scientific 46406 Alexa Fluor 488 C5 Maleimide Thermo Scientific A10254 Critical commercial |