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HEKA Elektronik pulse acquisition software
Pulse Acquisition Software, supplied by HEKA Elektronik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pulse+software/pulse+acquisition+software/pmc06101210-98-20-23
Average 90 stars, based on 1 article reviews
pulse acquisition software - by Bioz Stars, 2026-11
90/100 stars

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Patch Clamp:

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patchclamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: The mitochondrial Ca 2+ uniporter MCU is required for normal glucose-stimulated insulin secretion in vitro and in vivo
Article Snippet: .. Electrophysiological recordings were performed on single β-cells in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32 °C with normal saline solution (135mM NaCl, 5mM KCl, 1mM MgCl 2 , 1mM CaCl 2 , 10mM HEPES). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of 140mM KCl, 5mM MgCl 2 , 3.8mM CaCl 2 , 10mM HEPES, 10mM EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patch- clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: Chronic d -serine supplementation impairs insulin secretion
Article Snippet: .. Electrophysiological recordings were performed in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik). β-cells were identified morphologically and by depolarization of the membrane potential in response to 16.7 mM glucose. β-cells were constantly perfused at 32–33 °C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl 2 , 1 CaCl 2 , 10 HEPES, and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8–10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl 2 , 3.8 CaCl 2 , 10 HEPES, 10 EGTA (pH 7.2), and 20–25 μg/ml amphotericin B (Sigma–Aldrich).

Article Title: The Mitochondrial Ca 2+ Uniporter MCU Is Essential for Glucose-Induced ATP Increases in Pancreatic β-Cells
Article Snippet: .. Electrophysiological recordings and stimulation were done in whole-cell perforated-patch configuration, using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (HEKA Elektronik). ..

Article Title: Aromatic interactions with membrane modulate human BK channel activation
Article Snippet: .. We used inside-out patches to record Ionic currents with an Axopatch 200-B patch-clamp amplifier (Molecular Devices, Sunnyvale, CA) and ITC-18 interface and Pulse acquisition software (HEKA Elektronik GmbH, Holliston, MA). ..

Software:

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patchclamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: The mitochondrial Ca 2+ uniporter MCU is required for normal glucose-stimulated insulin secretion in vitro and in vivo
Article Snippet: .. Electrophysiological recordings were performed on single β-cells in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32 °C with normal saline solution (135mM NaCl, 5mM KCl, 1mM MgCl 2 , 1mM CaCl 2 , 10mM HEPES). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of 140mM KCl, 5mM MgCl 2 , 3.8mM CaCl 2 , 10mM HEPES, 10mM EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: Murine transgenic iPS cell line for monitoring and selection of cardiomyocytes.
Article Snippet: Cell membrane capacitancewas determined on-line using the Pulse program (Heka Elektronik). .. APs of spontaneously beating CMs were recorded by the whole-cell current-clamp technique using an EPC-9 amplifier and operated through the PULSE acquisition software (HEKAElektronik, Lambrecht, Germany). .. Response of CMs to hormonal regulation was assessed by administering isoproterenol (Iso) and carbachol (CCh) (Sigma-Aldrich).

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patch- clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: Chronic d -serine supplementation impairs insulin secretion
Article Snippet: .. Electrophysiological recordings were performed in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik). β-cells were identified morphologically and by depolarization of the membrane potential in response to 16.7 mM glucose. β-cells were constantly perfused at 32–33 °C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl 2 , 1 CaCl 2 , 10 HEPES, and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8–10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl 2 , 3.8 CaCl 2 , 10 HEPES, 10 EGTA (pH 7.2), and 20–25 μg/ml amphotericin B (Sigma–Aldrich).

Article Title: The L-type Ca 2+ Channels Blocker Nifedipine Represses Mesodermal Fate Determination in Murine Embryonic Stem Cells
Article Snippet: .. All recordings were performed using an EPC 9 amplifier and the Pulse acquisition software (Heka Elektronik, Lambrecht, Germany). ..

Article Title: The Mitochondrial Ca 2+ Uniporter MCU Is Essential for Glucose-Induced ATP Increases in Pancreatic β-Cells
Article Snippet: .. Electrophysiological recordings and stimulation were done in whole-cell perforated-patch configuration, using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (HEKA Elektronik). ..

Article Title: Aromatic interactions with membrane modulate human BK channel activation
Article Snippet: .. We used inside-out patches to record Ionic currents with an Axopatch 200-B patch-clamp amplifier (Molecular Devices, Sunnyvale, CA) and ITC-18 interface and Pulse acquisition software (HEKA Elektronik GmbH, Holliston, MA). ..

Membrane:

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patchclamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: The mitochondrial Ca 2+ uniporter MCU is required for normal glucose-stimulated insulin secretion in vitro and in vivo
Article Snippet: .. Electrophysiological recordings were performed on single β-cells in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32 °C with normal saline solution (135mM NaCl, 5mM KCl, 1mM MgCl 2 , 1mM CaCl 2 , 10mM HEPES). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of 140mM KCl, 5mM MgCl 2 , 3.8mM CaCl 2 , 10mM HEPES, 10mM EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patch- clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: Chronic d -serine supplementation impairs insulin secretion
Article Snippet: .. Electrophysiological recordings were performed in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik). β-cells were identified morphologically and by depolarization of the membrane potential in response to 16.7 mM glucose. β-cells were constantly perfused at 32–33 °C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl 2 , 1 CaCl 2 , 10 HEPES, and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8–10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl 2 , 3.8 CaCl 2 , 10 HEPES, 10 EGTA (pH 7.2), and 20–25 μg/ml amphotericin B (Sigma–Aldrich).

Saline:

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patchclamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: The mitochondrial Ca 2+ uniporter MCU is required for normal glucose-stimulated insulin secretion in vitro and in vivo
Article Snippet: .. Electrophysiological recordings were performed on single β-cells in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32 °C with normal saline solution (135mM NaCl, 5mM KCl, 1mM MgCl 2 , 1mM CaCl 2 , 10mM HEPES). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of 140mM KCl, 5mM MgCl 2 , 3.8mM CaCl 2 , 10mM HEPES, 10mM EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: The type 2 diabetes gene product STARD10 is a phosphoinositide-binding protein that controls insulin secretory granule biogenesis
Article Snippet: .. Electrophysiological recordings were performed in perforated patch- clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik, Pfalz, Germany). β-cells were identified morphologically and by depolarisation of the membrane potential in response to 17 mM glucose. β-cells were constantly perfused at 32°C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl2, 1 CaCl2, 10 HEPES and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8-10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl2, 3.8 CaCl2, 10 HEPES, 10 EGTA (pH 7.2) and 20–25 μg/ml amphotericin B (Sigma-Aldrich).

Article Title: Chronic d -serine supplementation impairs insulin secretion
Article Snippet: .. Electrophysiological recordings were performed in perforated patch-clamp configuration using an EPC9 patch-clamp amplifier controlled by Pulse acquisition software (Heka Elektronik). β-cells were identified morphologically and by depolarization of the membrane potential in response to 16.7 mM glucose. β-cells were constantly perfused at 32–33 °C with normal saline solution (mM): 135 NaCl, 5 KCl, 1 MgCl 2 , 1 CaCl 2 , 10 HEPES, and 16.7 glucose (pH 7.4). .. Recording electrodes had resistances of 8–10 MΩ and were filled with a solution comprised of (mM): 140 KCl, 5 MgCl 2 , 3.8 CaCl 2 , 10 HEPES, 10 EGTA (pH 7.2), and 20–25 μg/ml amphotericin B (Sigma–Aldrich).



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