Review



sgrna expression vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Addgene inc sgrna expression vector
    Sgrna Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pmc12911573-45-5-9
    Average 95 stars, based on 209 article reviews
    sgrna expression vector - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Construct:

    Article Title: TRIM56 Aggravates Cerebral Ischemia-Reperfusion Injury via Inhibiting KLF4-Activated Ferroptosis Signaling.
    Article Snippet: .. The pUC57-sgRNA (51132, Addgene) was used as the skeleton vector to construct the Trim56-sgRNA expression vector. .. First, mix the purified products of the Cas9 expression vector pST1374-Cas9 (44758, Addgene) and the sgRNA expression vector in vitro.

    Article Title: TRIM56 Aggravates Cerebral Ischemia‐Reperfusion Injury via Inhibiting KLF4‐Activated Ferroptosis Signaling
    Article Snippet: .. The pUC57‐sgRNA (51132, Addgene) was used as the skeleton vector to construct the Trim56‐sgRNA expression vector. .. First, mix the purified products of the Cas9 expression vector pST1374‐Cas9 (44758, Addgene) and the sgRNA expression vector in vitro.

    Expressing:

    Article Title: TRIM56 Aggravates Cerebral Ischemia-Reperfusion Injury via Inhibiting KLF4-Activated Ferroptosis Signaling.
    Article Snippet: .. The pUC57-sgRNA (51132, Addgene) was used as the skeleton vector to construct the Trim56-sgRNA expression vector. .. First, mix the purified products of the Cas9 expression vector pST1374-Cas9 (44758, Addgene) and the sgRNA expression vector in vitro.

    Article Title: SMYD2-mediated methylation of STAT1 protects against hepatic ischaemia/reperfusion injury by blocking JAK-STAT1 signalling pathways
    Article Snippet: .. The in vitro transcripts of sgRNA expression vector (pUC57-sgRNA, Addgene, 51132) and another expression vector (pST1374-Cas9, Addgene 44758) were purified, mixed and injected into single-cell fertilised eggs of C57BL/6 mice using the FemtoJet 5247 microinjection system. ..

    Article Title: RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis
    Article Snippet: SpyCas9 protein was obtained from IDT (Alt-R Cas9 Nuclease v3) or purified in-house as His-MBP-SpyCas9 (Supporting Information Figure S2 ). .. Immediately before use, the protein was diluted in PBS (Merk, Sigma-Aldrich). sgRNAs were designed, amplified, in vitro transcribed, and purified using the Precision gRNA Synthesis Kit (ThermoFisher Scientific, Invitrogen) as described in Supporting Information Scramble control sgRNA (Scr) was amplified before in vitro transcription from pUC57-sgRNA expression vector, a gift from Xingxu Huang (Addgene plasmid # 51132; http://n2t.net/addgene:51132 ; RRID:Addgene_51132) .. To be able to measure the change in melting curves before and after cleavage, we designed short synthetic dsDNA oligonucleotides (IDT) containing a target sequence as reporters of Cas9 activity.

    Article Title: TRIM56 Aggravates Cerebral Ischemia‐Reperfusion Injury via Inhibiting KLF4‐Activated Ferroptosis Signaling
    Article Snippet: .. The pUC57‐sgRNA (51132, Addgene) was used as the skeleton vector to construct the Trim56‐sgRNA expression vector. .. First, mix the purified products of the Cas9 expression vector pST1374‐Cas9 (44758, Addgene) and the sgRNA expression vector in vitro.

    CRISPR:

    Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication
    Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the BbsI sites of pUC19-sgRNA vector (Addgene #51132) that carries the guide-RNA scaffold sequence; Cas9 was expressed from pcDNA3.1-Cas9 (Addgene #41815, sequence-verified). .. The RFP cassette (714 bp) was amplified from pmRFP-C1 (Clontech) with primers RFP-fwd 5′-AATAGTAATCAATTACGGGGTCATT-3′ and RFP-rev 5′-AGATACATTGATGAGTTTGGACAAACCA-3′ (annealing 58 °C, 30 s) using Q5 HF polymerase (NEB).

    Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication.
    Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the BbsI sites of pUC19-sgRNA vector (Addgene #51132) that carries the guide-RNA scaffold sequence; Cas9 was expressed from pcDNA3.1-Cas9 (Addgene #41815, sequenceverified). .. The RFP cassette (714 bp) was amplified from pmRFP-C1 (Clontech) with primers RFP-fwd 5′-AATAGTAATCAATTACGGGG TCATT-3′ and RFP-rev 5′-AGATACATTGATGAGTTTGGACAAA CCA-3′ (annealing 58 °C, 30 s) using Q5 HF polymerase (NEB).

    Cloning:

    Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication
    Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the BbsI sites of pUC19-sgRNA vector (Addgene #51132) that carries the guide-RNA scaffold sequence; Cas9 was expressed from pcDNA3.1-Cas9 (Addgene #41815, sequence-verified). .. The RFP cassette (714 bp) was amplified from pmRFP-C1 (Clontech) with primers RFP-fwd 5′-AATAGTAATCAATTACGGGGTCATT-3′ and RFP-rev 5′-AGATACATTGATGAGTTTGGACAAACCA-3′ (annealing 58 °C, 30 s) using Q5 HF polymerase (NEB).

    Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication.
    Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the BbsI sites of pUC19-sgRNA vector (Addgene #51132) that carries the guide-RNA scaffold sequence; Cas9 was expressed from pcDNA3.1-Cas9 (Addgene #41815, sequenceverified). .. The RFP cassette (714 bp) was amplified from pmRFP-C1 (Clontech) with primers RFP-fwd 5′-AATAGTAATCAATTACGGGG TCATT-3′ and RFP-rev 5′-AGATACATTGATGAGTTTGGACAAA CCA-3′ (annealing 58 °C, 30 s) using Q5 HF polymerase (NEB).

    Sequencing:

    Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication
    Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the BbsI sites of pUC19-sgRNA vector (Addgene #51132) that carries the guide-RNA scaffold sequence; Cas9 was expressed from pcDNA3.1-Cas9 (Addgene #41815, sequence-verified). .. The RFP cassette (714 bp) was amplified from pmRFP-C1 (Clontech) with primers RFP-fwd 5′-AATAGTAATCAATTACGGGGTCATT-3′ and RFP-rev 5′-AGATACATTGATGAGTTTGGACAAACCA-3′ (annealing 58 °C, 30 s) using Q5 HF polymerase (NEB).

    Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication.
    Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the BbsI sites of pUC19-sgRNA vector (Addgene #51132) that carries the guide-RNA scaffold sequence; Cas9 was expressed from pcDNA3.1-Cas9 (Addgene #41815, sequenceverified). .. The RFP cassette (714 bp) was amplified from pmRFP-C1 (Clontech) with primers RFP-fwd 5′-AATAGTAATCAATTACGGGG TCATT-3′ and RFP-rev 5′-AGATACATTGATGAGTTTGGACAAA CCA-3′ (annealing 58 °C, 30 s) using Q5 HF polymerase (NEB).

    Plasmid Preparation:

    Article Title: The ultra-conserved lncRNA Crnde regulates neural differentiation by targeting Gbx2 during embryonic development of the thalamus.
    Article Snippet: 27 The thalamus regulates sensory processing, cognition, and sleep, yet the molecular 28 mechanisms underlying its development remain incompletely understood.. Long 29 noncoding RNAs (lncRNAs), particularly evolutionarily conserved ones, are highly 30 enriched in the brain.. Using public mRNA databases, we screen for lncRNAs with 31 embryonic brain expression and harboring ultraconserved non-coding elements 32 (UCNEs) in humans and mice, identifying colorectal neoplasia differentially 33 expressed (Crnde).

    Article Title: SMYD2-mediated methylation of STAT1 protects against hepatic ischaemia/reperfusion injury by blocking JAK-STAT1 signalling pathways
    Article Snippet: .. The in vitro transcripts of sgRNA expression vector (pUC57-sgRNA, Addgene, 51132) and another expression vector (pST1374-Cas9, Addgene 44758) were purified, mixed and injected into single-cell fertilised eggs of C57BL/6 mice using the FemtoJet 5247 microinjection system. ..

    Article Title: RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis
    Article Snippet: SpyCas9 protein was obtained from IDT (Alt-R Cas9 Nuclease v3) or purified in-house as His-MBP-SpyCas9 (Supporting Information Figure S2 ). .. Immediately before use, the protein was diluted in PBS (Merk, Sigma-Aldrich). sgRNAs were designed, amplified, in vitro transcribed, and purified using the Precision gRNA Synthesis Kit (ThermoFisher Scientific, Invitrogen) as described in Supporting Information Scramble control sgRNA (Scr) was amplified before in vitro transcription from pUC57-sgRNA expression vector, a gift from Xingxu Huang (Addgene plasmid # 51132; http://n2t.net/addgene:51132 ; RRID:Addgene_51132) .. To be able to measure the change in melting curves before and after cleavage, we designed short synthetic dsDNA oligonucleotides (IDT) containing a target sequence as reporters of Cas9 activity.

    Clone Assay:

    Article Title: The ultra-conserved lncRNA Crnde regulates neural differentiation by targeting Gbx2 during embryonic development of the thalamus.
    Article Snippet: 27 The thalamus regulates sensory processing, cognition, and sleep, yet the molecular 28 mechanisms underlying its development remain incompletely understood.. Long 29 noncoding RNAs (lncRNAs), particularly evolutionarily conserved ones, are highly 30 enriched in the brain.. Using public mRNA databases, we screen for lncRNAs with 31 embryonic brain expression and harboring ultraconserved non-coding elements 32 (UCNEs) in humans and mice, identifying colorectal neoplasia differentially 33 expressed (Crnde).

    In Vitro:

    Article Title: SMYD2-mediated methylation of STAT1 protects against hepatic ischaemia/reperfusion injury by blocking JAK-STAT1 signalling pathways
    Article Snippet: .. The in vitro transcripts of sgRNA expression vector (pUC57-sgRNA, Addgene, 51132) and another expression vector (pST1374-Cas9, Addgene 44758) were purified, mixed and injected into single-cell fertilised eggs of C57BL/6 mice using the FemtoJet 5247 microinjection system. ..

    Article Title: RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis
    Article Snippet: SpyCas9 protein was obtained from IDT (Alt-R Cas9 Nuclease v3) or purified in-house as His-MBP-SpyCas9 (Supporting Information Figure S2 ). .. Immediately before use, the protein was diluted in PBS (Merk, Sigma-Aldrich). sgRNAs were designed, amplified, in vitro transcribed, and purified using the Precision gRNA Synthesis Kit (ThermoFisher Scientific, Invitrogen) as described in Supporting Information Scramble control sgRNA (Scr) was amplified before in vitro transcription from pUC57-sgRNA expression vector, a gift from Xingxu Huang (Addgene plasmid # 51132; http://n2t.net/addgene:51132 ; RRID:Addgene_51132) .. To be able to measure the change in melting curves before and after cleavage, we designed short synthetic dsDNA oligonucleotides (IDT) containing a target sequence as reporters of Cas9 activity.

    Purification:

    Article Title: SMYD2-mediated methylation of STAT1 protects against hepatic ischaemia/reperfusion injury by blocking JAK-STAT1 signalling pathways
    Article Snippet: .. The in vitro transcripts of sgRNA expression vector (pUC57-sgRNA, Addgene, 51132) and another expression vector (pST1374-Cas9, Addgene 44758) were purified, mixed and injected into single-cell fertilised eggs of C57BL/6 mice using the FemtoJet 5247 microinjection system. ..

    Article Title: RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis
    Article Snippet: SpyCas9 protein was obtained from IDT (Alt-R Cas9 Nuclease v3) or purified in-house as His-MBP-SpyCas9 (Supporting Information Figure S2 ). .. Immediately before use, the protein was diluted in PBS (Merk, Sigma-Aldrich). sgRNAs were designed, amplified, in vitro transcribed, and purified using the Precision gRNA Synthesis Kit (ThermoFisher Scientific, Invitrogen) as described in Supporting Information Scramble control sgRNA (Scr) was amplified before in vitro transcription from pUC57-sgRNA expression vector, a gift from Xingxu Huang (Addgene plasmid # 51132; http://n2t.net/addgene:51132 ; RRID:Addgene_51132) .. To be able to measure the change in melting curves before and after cleavage, we designed short synthetic dsDNA oligonucleotides (IDT) containing a target sequence as reporters of Cas9 activity.

    Injection:

    Article Title: SMYD2-mediated methylation of STAT1 protects against hepatic ischaemia/reperfusion injury by blocking JAK-STAT1 signalling pathways
    Article Snippet: .. The in vitro transcripts of sgRNA expression vector (pUC57-sgRNA, Addgene, 51132) and another expression vector (pST1374-Cas9, Addgene 44758) were purified, mixed and injected into single-cell fertilised eggs of C57BL/6 mice using the FemtoJet 5247 microinjection system. ..

    Single Cell:

    Article Title: SMYD2-mediated methylation of STAT1 protects against hepatic ischaemia/reperfusion injury by blocking JAK-STAT1 signalling pathways
    Article Snippet: .. The in vitro transcripts of sgRNA expression vector (pUC57-sgRNA, Addgene, 51132) and another expression vector (pST1374-Cas9, Addgene 44758) were purified, mixed and injected into single-cell fertilised eggs of C57BL/6 mice using the FemtoJet 5247 microinjection system. ..

    Microinjection:

    Article Title: SMYD2-mediated methylation of STAT1 protects against hepatic ischaemia/reperfusion injury by blocking JAK-STAT1 signalling pathways
    Article Snippet: .. The in vitro transcripts of sgRNA expression vector (pUC57-sgRNA, Addgene, 51132) and another expression vector (pST1374-Cas9, Addgene 44758) were purified, mixed and injected into single-cell fertilised eggs of C57BL/6 mice using the FemtoJet 5247 microinjection system. ..

    Amplification:

    Article Title: RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis
    Article Snippet: SpyCas9 protein was obtained from IDT (Alt-R Cas9 Nuclease v3) or purified in-house as His-MBP-SpyCas9 (Supporting Information Figure S2 ). .. Immediately before use, the protein was diluted in PBS (Merk, Sigma-Aldrich). sgRNAs were designed, amplified, in vitro transcribed, and purified using the Precision gRNA Synthesis Kit (ThermoFisher Scientific, Invitrogen) as described in Supporting Information Scramble control sgRNA (Scr) was amplified before in vitro transcription from pUC57-sgRNA expression vector, a gift from Xingxu Huang (Addgene plasmid # 51132; http://n2t.net/addgene:51132 ; RRID:Addgene_51132) .. To be able to measure the change in melting curves before and after cleavage, we designed short synthetic dsDNA oligonucleotides (IDT) containing a target sequence as reporters of Cas9 activity.

    Control:

    Article Title: RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis
    Article Snippet: SpyCas9 protein was obtained from IDT (Alt-R Cas9 Nuclease v3) or purified in-house as His-MBP-SpyCas9 (Supporting Information Figure S2 ). .. Immediately before use, the protein was diluted in PBS (Merk, Sigma-Aldrich). sgRNAs were designed, amplified, in vitro transcribed, and purified using the Precision gRNA Synthesis Kit (ThermoFisher Scientific, Invitrogen) as described in Supporting Information Scramble control sgRNA (Scr) was amplified before in vitro transcription from pUC57-sgRNA expression vector, a gift from Xingxu Huang (Addgene plasmid # 51132; http://n2t.net/addgene:51132 ; RRID:Addgene_51132) .. To be able to measure the change in melting curves before and after cleavage, we designed short synthetic dsDNA oligonucleotides (IDT) containing a target sequence as reporters of Cas9 activity.



    Similar Products

    99
    New England Biolabs puc57 vector 357
    Puc57 Vector 357, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/EcoRI/10__1016_slash_j__omtn__2025__102809-135-8-14
    Average 99 stars, based on 1 article reviews
    puc57 vector 357 - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Sangon Biotech puc57 vectors
    Puc57 Vectors, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/orf1+pcv2+pcv2+plasmid+pmd18+positive/pm42243997-82-19-22
    Average 86 stars, based on 1 article reviews
    puc57 vectors - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Sangon Biotech puc57 vector
    Puc57 Vector, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/orf1+pcv2+pcv2+plasmid+pmd18+positive/pm41812557-90-29-16
    Average 86 stars, based on 1 article reviews
    puc57 vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Addgene inc puc57 sgrna 647
    Puc57 Sgrna 647, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pm41655632-259-15-17
    Average 95 stars, based on 1 article reviews
    puc57 sgrna 647 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Addgene inc sgrna expression vector
    Sgrna Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pmc12911573-45-5-9
    Average 95 stars, based on 1 article reviews
    sgrna expression vector - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Azenta puc57 kan gw ok vector
    Puc57 Kan Gw Ok Vector, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/puc57/pmc13001375-55-13-16
    Average 86 stars, based on 1 article reviews
    puc57 kan gw ok vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Addgene inc bbsi sites
    Bbsi Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pm41383737-67-16-21
    Average 95 stars, based on 1 article reviews
    bbsi sites - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Addgene inc puc57 sgrna
    Puc57 Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pmc12884795-207-1-3
    Average 95 stars, based on 1 article reviews
    puc57 sgrna - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results