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ptt5 c1 based expression vectors  (Addgene inc)


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    Structured Review

    Addgene inc ptt5 c1 based expression vectors
    Ptt5 C1 Based Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptt5+expression+vector/pTT5-hSDC1-N20+(Plasmid+%2352355)/pm39856351-312-4-7
    Average 93 stars, based on 4 article reviews
    ptt5 c1 based expression vectors - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends
    Article Snippet: .. To overexpress proteins in HEK293T cells, cDNAs of the human proteins were cloned into pTT5-based expression vectors (#52355; Addgene), which also had a SII and fluorescent tags (GFP or mCherry) to generate SII-GFP-CP110, CEP97-GFP-SII, CEP97^CP110-GFP-SII, CPAP-N WT -mCherry-SII, and CPAP-N MUT -mCherry-SII. ..

    Article Title: Microtubule plus-end regulation by centriolar cap proteins
    Article Snippet: .. To overexpress proteins in HEK293T cells, cDNAs of the human proteins were cloned into pTT5 based expression vectors (Addgene #52355). ..

    Article Title: Centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends.
    Article Snippet: .. To overexpress proteins in HEK293T cells, cDNAs of the human proteins were cloned into pTT5-based expression vectors (#52355; Addgene), which also had a SII and fluorescent tags (GFP or mCherry) to generate SII-GFP-CP110, CEP97-GFP-SII, CEP97^CP110-GFP-SII, CPAP-NWT-mCherry-SII, and CPAPNMUT-mCherry-SII. ..

    Article Title: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth
    Article Snippet: All proteins except CEP104 were cloned into modified pEGFP-C1 or pmCherry-C1 vectors with a StrepII tag and expressed in HEK293T cells (American Type Culture Collection, CRL-3216). .. CEP104 was cloned into pTT5-C1-based expression vectors (Addgene, 52355), which also had a StrepII tag and fluorescent tags (GFP or Cherry). ..

    Article Title: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth.
    Article Snippet: DNA constructs, cell lines and cell culture All proteins except CEP104 were cloned into modified pEGFP-C1 or pmCherry-C1 vectors with a StrepII tag and expressed in HEK293T cells (American Type Culture Collection, CRL-3216). .. CEP104 was cloned into pTT5-C1-based expression vectors (Addgene, 52355), which also had a StrepII tag and fluorescent tags (GFP or Cherry). ..

    Expressing:

    Article Title: Centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends
    Article Snippet: .. To overexpress proteins in HEK293T cells, cDNAs of the human proteins were cloned into pTT5-based expression vectors (#52355; Addgene), which also had a SII and fluorescent tags (GFP or mCherry) to generate SII-GFP-CP110, CEP97-GFP-SII, CEP97^CP110-GFP-SII, CPAP-N WT -mCherry-SII, and CPAP-N MUT -mCherry-SII. ..

    Article Title: Microtubule plus-end regulation by centriolar cap proteins
    Article Snippet: .. To overexpress proteins in HEK293T cells, cDNAs of the human proteins were cloned into pTT5 based expression vectors (Addgene #52355). ..

    Article Title: Aurora B controls microtubule stability to regulate abscission dynamics in stem cells.
    Article Snippet: .. The transfectionmix was added on top of the cells and the cells were placed in the incubator overnight, and typically rinsed the nextmorning and imaged in the afternoon. pTT5 SII-GFP-MCAK (a kind gift from Joyce Meiring, Akhmanova lab) was obtained by cloning cDNAs of the human proteins into pTT5 based expression vectors (Addgene #52355), which also has a Twin-Strep-tag (SII) and a GFP tag. ..

    Article Title: Centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends.
    Article Snippet: .. To overexpress proteins in HEK293T cells, cDNAs of the human proteins were cloned into pTT5-based expression vectors (#52355; Addgene), which also had a SII and fluorescent tags (GFP or mCherry) to generate SII-GFP-CP110, CEP97-GFP-SII, CEP97^CP110-GFP-SII, CPAP-NWT-mCherry-SII, and CPAPNMUT-mCherry-SII. ..

    Article Title: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth
    Article Snippet: All proteins except CEP104 were cloned into modified pEGFP-C1 or pmCherry-C1 vectors with a StrepII tag and expressed in HEK293T cells (American Type Culture Collection, CRL-3216). .. CEP104 was cloned into pTT5-C1-based expression vectors (Addgene, 52355), which also had a StrepII tag and fluorescent tags (GFP or Cherry). ..

    Article Title: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth.
    Article Snippet: DNA constructs, cell lines and cell culture All proteins except CEP104 were cloned into modified pEGFP-C1 or pmCherry-C1 vectors with a StrepII tag and expressed in HEK293T cells (American Type Culture Collection, CRL-3216). .. CEP104 was cloned into pTT5-C1-based expression vectors (Addgene, 52355), which also had a StrepII tag and fluorescent tags (GFP or Cherry). ..

    Cloning:

    Article Title: Aurora B controls microtubule stability to regulate abscission dynamics in stem cells.
    Article Snippet: .. The transfectionmix was added on top of the cells and the cells were placed in the incubator overnight, and typically rinsed the nextmorning and imaged in the afternoon. pTT5 SII-GFP-MCAK (a kind gift from Joyce Meiring, Akhmanova lab) was obtained by cloning cDNAs of the human proteins into pTT5 based expression vectors (Addgene #52355), which also has a Twin-Strep-tag (SII) and a GFP tag. ..



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