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protoscript ii first strand cdna synthesis kit  (New England Biolabs)


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    Structured Review

    New England Biolabs protoscript ii first strand cdna synthesis kit
    Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pm42129231-419-14-21
    Average 99 stars, based on 1832 article reviews
    protoscript ii first strand cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Detection, persistence, and rising prevalence of oncogenic viruses revealed by wastewater metagenomics.
    Article Snippet: After bead beating, samples were centrifuged at 14–17 × 1,000 RPM for 2 min. Once centrifuged, the DNA and RNA were extracted using the Qiagen QIAamp VIRAL RNA Mini Kit. .. Reverse transcription of RNA to cDNA was first carried out with the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), NEBNext Ultra II Non-Direc tional RNA Second Strand Module (New England Biolabs Inc.), and Random Primer 6 (New England Biolabs Inc.) before mixing it with DNA for library construction. .. Reverse transcription of RNA to cDNA was first carried out with the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), NEBNext Ultra II Non-Direc tional RNA Second Strand Module (New England Biolabs Inc.), and Random Primer 6 (New England Biolabs Inc.) before mixing it with DNA for library construction.

    Article Title: Functional Characterization of OTU Domain-Containing Deubiquitinases from Plant Pathogenic Fungi Reveals Distinct Immune Modulatory Mechanisms
    Article Snippet: .. First-strand cDNA was synthesized from 750 ng of total RNA using random primers and reverse transcriptase (ProtoScript II First Strand cDNA Synthesis Kit, NEB, Ipswich, MA, USA). ..

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. For gene expression analysis by reverse transcription quantitative PCR (RT-qPCR), 1 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) using oligo-dT primers to ensure selective reverse transcription of mRNA. .. RT-qPCR was performed on a Opus qPCR CFX384 detection system (Bio-Rad, 12011452) using the NZYSpeedy qPCR Green Master Mix (2×) (NZYtech, MB224).

    Article Title: Premature cleavage and polyadenylation in the minor intron of PTEN modulate its expression and generate a functional long non-coding RNA
    Article Snippet: Protein concentrations were measured using the Bio-Rad protein assay. .. For reverse transcription, 1 μg of RNA was converted to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs), according to the manufacturer’s protocol. cDNA was then diluted 10-fold in nuclease-free water and used as input for PCR. ..

    cDNA Synthesis:

    Article Title: Detection, persistence, and rising prevalence of oncogenic viruses revealed by wastewater metagenomics.
    Article Snippet: After bead beating, samples were centrifuged at 14–17 × 1,000 RPM for 2 min. Once centrifuged, the DNA and RNA were extracted using the Qiagen QIAamp VIRAL RNA Mini Kit. .. Reverse transcription of RNA to cDNA was first carried out with the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), NEBNext Ultra II Non-Direc tional RNA Second Strand Module (New England Biolabs Inc.), and Random Primer 6 (New England Biolabs Inc.) before mixing it with DNA for library construction. .. Reverse transcription of RNA to cDNA was first carried out with the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), NEBNext Ultra II Non-Direc tional RNA Second Strand Module (New England Biolabs Inc.), and Random Primer 6 (New England Biolabs Inc.) before mixing it with DNA for library construction.

    Article Title: Pampa biome plant extracts and flavonoids show antiviral activity against Bovine Herpesvirus and Bovine Viral Diarrhea Virus.
    Article Snippet: The final RNA was eluted in 30 μL of ultrapure sterile water (RMBIO) and quantified using a NanoDropTM One Spectrophotometer (Thermo ScientificTM). .. Samples were stored at –80 °C until use. cDNA synthesis was performed using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs Inc.), following the manufacturer’s instructions. ..

    Article Title: Prospective metagenomic sequencing of wastewater across the United States yields robust viral enrichment and concordance with digital PCR measurements
    Article Snippet: .. cDNA synthesis was performed with ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs #E6560L, Ipswich, MA, USA) using the neat nucleic acid as template. .. cDNA was purified using Kapa HyperPure Beads (Roche, Basel, Switzerland), and the cDNA concentration was measured using Qubit (Thermo Fisher).

    Article Title: Functional Characterization of OTU Domain-Containing Deubiquitinases from Plant Pathogenic Fungi Reveals Distinct Immune Modulatory Mechanisms
    Article Snippet: .. First-strand cDNA was synthesized from 750 ng of total RNA using random primers and reverse transcriptase (ProtoScript II First Strand cDNA Synthesis Kit, NEB, Ipswich, MA, USA). ..

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. For gene expression analysis by reverse transcription quantitative PCR (RT-qPCR), 1 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) using oligo-dT primers to ensure selective reverse transcription of mRNA. .. RT-qPCR was performed on a Opus qPCR CFX384 detection system (Bio-Rad, 12011452) using the NZYSpeedy qPCR Green Master Mix (2×) (NZYtech, MB224).

    Article Title: Premature cleavage and polyadenylation in the minor intron of PTEN modulate its expression and generate a functional long non-coding RNA
    Article Snippet: Protein concentrations were measured using the Bio-Rad protein assay. .. For reverse transcription, 1 μg of RNA was converted to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs), according to the manufacturer’s protocol. cDNA was then diluted 10-fold in nuclease-free water and used as input for PCR. ..

    Article Title: Insights from healthy mares reveal that mammalian uteri harbor a diverse virome.
    Article Snippet: Nucleic acid quantification was done using a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Amplified samples were kept at 4°C for short-term storage. cDNA Synthesis and Library Preparation Up to 1 μg template RNA and ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA) were used in first strand cDNA synthesis following kit protocols. .. The second strand cDNA synthesis was done using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs Inc.) according to the kit protocols.

    Article Title: Proteomic profiling of ANO10-deficient SH-SY5Y cells reveals candidate pathways relevant to SCAR10.
    Article Snippet: .. Total RNA was extracted from edited cells using the NucleoSpin Mini Kit (Macherey Nagel GmbH & Co KG, Duren, Germany), and cDNA was synthesised with the ProtoScript First Strand cDNA Synthesis Kit (NEB) according to the manufacturer’s instructions. .. Three biological replicates were analysed per sample, each run in triplicate. qPCR was performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific), and data were analysed using the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.3 (Applied Biosystems).

    Synthesized:

    Article Title: Functional Characterization of OTU Domain-Containing Deubiquitinases from Plant Pathogenic Fungi Reveals Distinct Immune Modulatory Mechanisms
    Article Snippet: .. First-strand cDNA was synthesized from 750 ng of total RNA using random primers and reverse transcriptase (ProtoScript II First Strand cDNA Synthesis Kit, NEB, Ipswich, MA, USA). ..

    Gene Expression:

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. For gene expression analysis by reverse transcription quantitative PCR (RT-qPCR), 1 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) using oligo-dT primers to ensure selective reverse transcription of mRNA. .. RT-qPCR was performed on a Opus qPCR CFX384 detection system (Bio-Rad, 12011452) using the NZYSpeedy qPCR Green Master Mix (2×) (NZYtech, MB224).

    Real-time Polymerase Chain Reaction:

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. For gene expression analysis by reverse transcription quantitative PCR (RT-qPCR), 1 μg of RNA was reverse-transcribed into complementary DNA (cDNA) using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) using oligo-dT primers to ensure selective reverse transcription of mRNA. .. RT-qPCR was performed on a Opus qPCR CFX384 detection system (Bio-Rad, 12011452) using the NZYSpeedy qPCR Green Master Mix (2×) (NZYtech, MB224).

    Polymerase Chain Reaction:

    Article Title: Premature cleavage and polyadenylation in the minor intron of PTEN modulate its expression and generate a functional long non-coding RNA
    Article Snippet: Protein concentrations were measured using the Bio-Rad protein assay. .. For reverse transcription, 1 μg of RNA was converted to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs), according to the manufacturer’s protocol. cDNA was then diluted 10-fold in nuclease-free water and used as input for PCR. ..

    Amplification:

    Article Title: Insights from healthy mares reveal that mammalian uteri harbor a diverse virome.
    Article Snippet: Nucleic acid quantification was done using a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). .. Amplified samples were kept at 4°C for short-term storage. cDNA Synthesis and Library Preparation Up to 1 μg template RNA and ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA) were used in first strand cDNA synthesis following kit protocols. .. The second strand cDNA synthesis was done using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs Inc.) according to the kit protocols.



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