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protein arrays  (Thermo Fisher)


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    Structured Review

    Thermo Fisher protein arrays
    Protein Arrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoarray+human+protein+microarray+v5+0/Tween+20/pmc06210676-96-21-30
    Average 99 stars, based on 1 article reviews
    protein arrays - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Proteolysis Targeting Chimera (PROTAC) Linkerology Enhances the CDK-Degradation Selectivity Profile of a Multi-CDK Inhibitor: Discovery of Potent, Anti-Leukemic CDK9 Degraders
    Article Snippet: .. The liganded beads were blocked with excess biotin and washed with blocking buffer (SeaBlock (Pierce), 1% BSA, 0.05% Tween 20, 1 mM DTT) to remove the unbound ligand and to reduce non-specific binding. ..

    Article Title: Allosteric inhibition of human mitochondrial ClpP by hijacking the dordaviprone (ONC201) binding site
    Article Snippet: .. The liganded beads were blocked with excess biotin and washed with blocking buffer (SeaBlock (Pierce), 1% BSA, 0.05% Tween 20, 1mM DTT) to remove unbound ligand and to reduce nonspecific phage binding. ..

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to separate lysates with 4-12% Bis-Tris gels (Thermo Scientific) before transfer to PVDF membranes using an iBlot3 (Invitrogen). .. Blocking with 5% BSA in Tris-buffered saline with Tween 20 (TBST) for 1 h at room temperature was followed by overnight incubation with primary antibodies diluted in SuperBlock blocking buffers (Thermo Fisher Scientific) at 4 °C. ..

    Nonspecific Binding:

    Article Title: Proteolysis Targeting Chimera (PROTAC) Linkerology Enhances the CDK-Degradation Selectivity Profile of a Multi-CDK Inhibitor: Discovery of Potent, Anti-Leukemic CDK9 Degraders
    Article Snippet: .. The liganded beads were blocked with excess biotin and washed with blocking buffer (SeaBlock (Pierce), 1% BSA, 0.05% Tween 20, 1 mM DTT) to remove the unbound ligand and to reduce non-specific binding. ..

    Membrane:

    Article Title: PIK3CA mutant cervical cancer is selectively suppressed by PI3Kα inhibition (Alpelisib/BYL-719 and Inavolisib/GDC-0077) and cooperates with HPV directed T cell therapy
    Article Snippet: According to the manufacturer's protocols, whole-cell protein lysates were prepared from cell lines using RIPA lysis buffer supplemented with Complete Protease Inhibitor Cocktail (Santa Cruz, CA, United States). .. In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% BSA at 4°C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution from1:250 to 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666).

    Saline:

    Article Title: PIK3CA mutant cervical cancer is selectively suppressed by PI3Kα inhibition (Alpelisib/BYL-719 and Inavolisib/GDC-0077) and cooperates with HPV directed T cell therapy
    Article Snippet: According to the manufacturer's protocols, whole-cell protein lysates were prepared from cell lines using RIPA lysis buffer supplemented with Complete Protease Inhibitor Cocktail (Santa Cruz, CA, United States). .. In total, 20 - 40 micrograms of whole cell protein were separated in a 4–12% NuPAGE Bis-Tris-gel, transferred to PVDF membrane (Thermo Fisher Scientific, Carlsbad, CA, United States), and immunoblotted with primary antibodies in 0.05% Tween 20-Tris-buffered saline (TBST) containing 5% BSA at 4°C with shaking overnight. .. The primary antibodies used in this study were: rabbit monoclonal anti-PD-L1, (Cell Signaling Technology Cat# 13684, RRID: AB_2687655), YAP1, (Cell Signaling Technology Cat# 14074, RRID: AB_2650491), EGFR (Cell Signaling Technology Cat# 4267, RRID: AB_2800085), CTGF (Cell Signaling Technology Cat# 86641, RRID: AB_2800085), Integrin α6 (Cell Signaling Technology Cat# 3750, RRID: AB_2249263), IRF1 (Cell Signaling Technology Cat# 8478, RRID: AB_10949108) at a dilution from1:250 to 1:500; mouse monoclonal anti-HPV16 E7 at 1:100 (Santa Cruz Cat# sc-51951, RRID: AB_629662), and goat monoclonal anti-p16 at 1:500 (R&D system Cat# AF5779, RRID: AB_1964666).

    Article Title: Inhibition of WNT signaling sensitizes CRC cells to anti-tumor effects of retinoids: basis for new drug combinations in oncology
    Article Snippet: For each sample, cell lysates containing 40 μg of protein were loaded to 4%–20% SDS‒PAGE gel (Bio-Rad, Hercules, CA, USA) for electrophoresis at 200 volts for approximately 40–45 min, followed by transferring at 100 volts for 75–80 min to a 0.45 μm PVDF film (Thermo Fisher Scientific, Waltham, MA, USA). .. Blots were blocked in BLOTTO solution freshly prepared with 5% nonfat dry milk powder in 1 × Tris-buffered saline (Bio-Rad, Hercules, CA, USA) with 0.1% Tween 20 (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h, before incubation in primary antibodies diluted in 5% BLOTTO on a rocking platform at 4 °C overnight. ..

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to separate lysates with 4-12% Bis-Tris gels (Thermo Scientific) before transfer to PVDF membranes using an iBlot3 (Invitrogen). .. Blocking with 5% BSA in Tris-buffered saline with Tween 20 (TBST) for 1 h at room temperature was followed by overnight incubation with primary antibodies diluted in SuperBlock blocking buffers (Thermo Fisher Scientific) at 4 °C. ..

    Incubation:

    Article Title: Inhibition of WNT signaling sensitizes CRC cells to anti-tumor effects of retinoids: basis for new drug combinations in oncology
    Article Snippet: For each sample, cell lysates containing 40 μg of protein were loaded to 4%–20% SDS‒PAGE gel (Bio-Rad, Hercules, CA, USA) for electrophoresis at 200 volts for approximately 40–45 min, followed by transferring at 100 volts for 75–80 min to a 0.45 μm PVDF film (Thermo Fisher Scientific, Waltham, MA, USA). .. Blots were blocked in BLOTTO solution freshly prepared with 5% nonfat dry milk powder in 1 × Tris-buffered saline (Bio-Rad, Hercules, CA, USA) with 0.1% Tween 20 (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h, before incubation in primary antibodies diluted in 5% BLOTTO on a rocking platform at 4 °C overnight. ..

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. Cells were resuspended in Cell Lysis Buffer [25 mM KCl (Sigma–Aldrich, 27810.295), 5 mM TRIS (Sigma–Aldrich, 0497–5KG) pH 8.3, 1.25 mM MgCl 2 (VWR BDH7899-1), 0.225% IGEPAL (Sigma–Aldrich, I8896-50 ML), and 0.225% Tween20 (VWR)] supplemented with 0.4 μg/μl Proteinase K (Thermo Fisher Scientific, EO0492), vortexed, and incubated sequentially at 65°C for 6 min and 95°C for 2 min. Lysates were cooled down, and 1–2 μl were used directly for polymerase chain reaction (PCR). .. For applications requiring high-purity gDNA, including Sanger sequencing, gDNA was isolated using the NZY Tissue gDNA Isolation Kit (NZYTech, MB13503) according to the manufacturer’s instructions.

    Article Title: Distinct hyaluronan content and hierarchical organization of collagen-elastic fiber assemblies in subcutaneous fascia
    Article Snippet: .. Samples were incubated with elastin monoclonal antibody BA-4 (MA1-27129, Thermo Fisher, Waltham, MA, USA; dilution 1: 100 in T-PBS containing 0.1% BSA) for 1 h at room temperature, washed three times for 5 min each with T-PBS (0.1% Tween-20 in PBS), and then reacted with Alexa Fluor 594–conjugated goat anti-mouse IgG (A11032; Thermo Fisher; dilution 1:1000 in T-PBS containing 0.1% BSA) for 1 h at room temperature [ ]. .. According to the manufacturer's protocol, 20 μM CHP (FLU60; 3Helix/Funakoshi) dissolved in PBS was heated at 80 °C for 5 min, rapidly cooled in an ice bath, and centrifuged; the supernatant was then used without further dilution [ , , ].

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to separate lysates with 4-12% Bis-Tris gels (Thermo Scientific) before transfer to PVDF membranes using an iBlot3 (Invitrogen). .. Blocking with 5% BSA in Tris-buffered saline with Tween 20 (TBST) for 1 h at room temperature was followed by overnight incubation with primary antibodies diluted in SuperBlock blocking buffers (Thermo Fisher Scientific) at 4 °C. ..

    Lysis:

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. Cells were resuspended in Cell Lysis Buffer [25 mM KCl (Sigma–Aldrich, 27810.295), 5 mM TRIS (Sigma–Aldrich, 0497–5KG) pH 8.3, 1.25 mM MgCl 2 (VWR BDH7899-1), 0.225% IGEPAL (Sigma–Aldrich, I8896-50 ML), and 0.225% Tween20 (VWR)] supplemented with 0.4 μg/μl Proteinase K (Thermo Fisher Scientific, EO0492), vortexed, and incubated sequentially at 65°C for 6 min and 95°C for 2 min. Lysates were cooled down, and 1–2 μl were used directly for polymerase chain reaction (PCR). .. For applications requiring high-purity gDNA, including Sanger sequencing, gDNA was isolated using the NZY Tissue gDNA Isolation Kit (NZYTech, MB13503) according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. Cells were resuspended in Cell Lysis Buffer [25 mM KCl (Sigma–Aldrich, 27810.295), 5 mM TRIS (Sigma–Aldrich, 0497–5KG) pH 8.3, 1.25 mM MgCl 2 (VWR BDH7899-1), 0.225% IGEPAL (Sigma–Aldrich, I8896-50 ML), and 0.225% Tween20 (VWR)] supplemented with 0.4 μg/μl Proteinase K (Thermo Fisher Scientific, EO0492), vortexed, and incubated sequentially at 65°C for 6 min and 95°C for 2 min. Lysates were cooled down, and 1–2 μl were used directly for polymerase chain reaction (PCR). .. For applications requiring high-purity gDNA, including Sanger sequencing, gDNA was isolated using the NZY Tissue gDNA Isolation Kit (NZYTech, MB13503) according to the manufacturer’s instructions.

    Binding Assay:

    Article Title: Allosteric inhibition of human mitochondrial ClpP by hijacking the dordaviprone (ONC201) binding site
    Article Snippet: .. The liganded beads were blocked with excess biotin and washed with blocking buffer (SeaBlock (Pierce), 1% BSA, 0.05% Tween 20, 1mM DTT) to remove unbound ligand and to reduce nonspecific phage binding. ..



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