protoarray human protein micro array v5 0 (Thermo Fisher)

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Thermo Fisher protoarray human protein micro array v5 0

Auto-Ab identification methods used in this study. ( A ) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. ( b ) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). ( C ) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in <t>ProtoArray</t> and AlphaScreen systems. ( D ) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.

Protoarray Human Protein Micro Array V5 0, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protoarray+human+protein+microarray+v5+0/Collagen/pmc04539100-139-7-12
Average 99 stars, based on 1 article reviews

protoarray human protein micro array v5 0 - by Bioz Stars, 2026-10

99/100 stars

Images

1) Product Images from "Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD"

Article Title: Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD

Journal: Biomarker Insights

doi: 10.4137/BMI.S28209

Figure Legend Snippet: Auto-Ab identification methods used in this study. ( A ) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. ( b ) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). ( C ) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. ( D ) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.

Techniques Used: Immunofluorescence, Amplified Luminescent Proximity Homogenous Assay, Enzyme-linked Immunosorbent Assay

Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with ( A ) ProtoArray and ( b ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between ( C ) ProtoArray and ( D ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).

Figure Legend Snippet: Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with ( A ) ProtoArray and ( b ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between ( C ) ProtoArray and ( D ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).

Techniques Used: Amplified Luminescent Proximity Homogenous Assay

Concentration Assay:

Article Title: Substituted 6,7-dihydro-5H-benzo[7]annulene compounds and their derivatives, processes for their preparation and therapeutic uses thereof
Article Snippet: .. MCF7 cells (ATCC) were seeded in 384 wells microplate (collagen coated) at a concentration of 10000 cells/30 μL per well in red phenol free MEM alpha medium (invitrogen) containing 5% charcoal dextran striped FBS. ..

Staining:

Article Title: Shexiang Tongxin Dropping Pills targeting Piezo1/Ca 2+ /NLRP3 axis attenuates vascular endothelial inflammation
Article Snippet: .. Antibodies used include: Anti-Piezo1 (DF12083, Affinity, Changzhou, China), ICAM-1 (sc-107, Santa, USA)、VCAM-1 (sc-13160, Santa, USA), NLRP3 (AF4620, Affinity, China), NLRP3 (45889, Novus, China), mature-IL-1β (WL00891, Wanlei, China), F4/80(A18637, ABclonal, China), Pro-IL-1β (WL02257, Wanlei, China), Caspase-1 (PA5-87536, Invitrogen, USA), GAPDH (60004-1-1G, Proteintech, China), Anti-mouse (7076 s, CST, USA), Anti-rabbit (7074 s, CST, USA), Donkey-anti-Sheep 555 (A21436, Invitrogen, USA), Donkey-anti-Rabbit 488 (A21206, Invitrogen, USA), Donkey-anti-mouse 488 (A21202, Invitrogen, USA), Donkey-anti-mouse 555 ( A31570 , Invitrogen, USA), Collagen fiber and elastic fiber staining kit (G1597, Solarbio, China), ELISA kits (IL-18: SEKM-0019, IL-1β: SEKM-0002, Solarbio, China). ..

Enzyme-linked Immunosorbent Assay:

Cell Culture:

Article Title: RAB27A silencing modulates miR-30d-5p expression and extracellular vesicle non-coding RNA cargo in diabetic podocytes.
Article Snippet: .. Cells were cultured on collagen type I-coated (Gibco, Thermo-Fisher Scientific, Waltham, MA, USA) flasks or plates in the Roswell Park Memorial Institute (RPMI) medium 1640 supplemented with 10% fetal bovine serum (FBS), 1% penicillinstreptomycin (both from Biowest, Nuaillé, France), and 1% insulin-transferrinselenium (ITS, Gibco, Thermo-Fisher Scientific, Waltham, MA, USA). ..

other:

Article Title: Platinum Cross-linked Collagen Matrices with Tunable Stiffness as a Platform to Investigate Cellular Mechanosensing
Article Snippet: Platinum concentrations were determined from a standard curve generated using a platinum standard solution (Fujifilm Wako Pure Chemical).

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