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protoarray human protein microarray v5 0  (Thermo Fisher)


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    Thermo Fisher protoarray human protein microarray v5 0
    Protoarray Human Protein Microarray V5 0, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoarray+human+protein+microarray+v5+0/HEPES/pm34949741-46-1-6
    Average 99 stars, based on 1 article reviews
    protoarray human protein microarray v5 0 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Staining:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure
    Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM HEPES (ThermoFisher Scientific), pH = 7.4, cultured at 37 °C and 5% CO 2 with the addition of 25 μmol/L of cytochalasin D (Cayman) in the media for 48 h. 10 μg/ml Hoechst 33258 (Invitrogen, H3569), a DNA-binding dye was utilized to stain cardiomyocytes nuclei. ..

    Polymerase Chain Reaction:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Western Blot:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Recombinant:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    Software:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: S33102 HEPES Merck Cat#: H3375 MgCl2 Merck Cat#: M8266 CaCl2 Merck Cat#:449709 Critical commercial assays NucleoSpin Gel and PCR Clean-up Kit Macherey-Nagel Cat#: 740609.250 Deposited data Original western blot images Mendeley: https://doi.org/10.17632/ wccscb4vt3.1 N/A Experimental models: Cell lines W8 immortalized mouse embryonic fibroblasts Jenuwein lab W8 D5 immortalized mouse embryonic fibroblasts Jenuwein lab D5 NIH 3T3 HP1α-EGFP immortalized mouse embryonic fibroblasts Jenuwein lab N/A Oligonucleotides MSR_for (5’-TGGAATATGGC GAGAAAACTG) Ching et al.73 N/A MSR_rev (5’-AGGTCCTTCAG TGGGCATTT) Ching et al.73 N/A Recombinant DNA pCAGGS-Suv39h1-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #693 pCAGGS-Suv39h2-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #694 pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro Velazquez et al.8 Laboratory of Thomas Jenuwein Database: #703 pCAGGS-BD-Suv39h1-EGFP-IRES-Puro This study Laboratory of Thomas Jenuwein Database: #696 Software and algorithms R (v4.4.3) R Core Team https://www.r-project.org/ Clustal Omega EMBL-EBI https://www.ebi.ac.uk/Tools/msa/clustalo/ GraphPad Prism 10 GraphPad Software https://www.graphpad.com/ Peptides (v2.4.6) Charif & Lobry, 2007 https://cran.r-project.org/package=Peptides ggplot2 (v3.5.1) Hadley Wickham https://cran.r-project.org/package=ggplot2 ggrepel (v0.9.6) Slowikowski et al. https://cran.r-project.org/package=ggrepel IUPred2 Dosztányi et al., 2005 https://iupred2a.elte.hu/ Zen Black software Zeiss https://www.zeiss.com/microscopy/ int/products/microscope-software/zen.html Fiji (v2.9.0) Schindelin et al., 2012 https://fiji.sc/ Marvin JS (22.11.1) Chemaxon internal http://www.chemaxon.com e2 iScience 29, 115626, May 15, 2026 ..

    other:

    Article Title: Establishment of a broiler jejunal organoid model based on optimized activators or optimized two-step media and its assessment
    Article Snippet: Media compositions were as follows: CMDM: 50% ( v / v ) CM, 37% ( v / v ) Advanced DMEM/F12 (12634010, Gibco, NY, USA), 10% ( v / v ) fetal bovine serum ( FBS ; 16000044, Gibco, NY, USA), 1% ( v/v ) penicillin / streptomycin ( P/S , 15140122, Gibco, NY, USA), 1% ( v / v ) Glutamax (200 mM, 35050061, Gibco, NY, USA), 1% ( v / v ) HEPES (1 M, 15630106, Gibco, NY, USA), 1 mM N-Acetyl- l -Cysteine ( NAC , A7250, Sigma, Tokyo, Japan), 10 μM Y-27632 2HCl (1254, R&D System, MN, USA), 10 μM SB202190 (1614, R&D System, MN, USA), 0.5 μM A83-01 (SML0788, Sigma, Tokyo, Japan), and 50 ng/mL EGF (PMG8043, Gibco, NY, USA).

    Isolation:

    Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure
    Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM HEPES (ThermoFisher Scientific), pH = 7.4, cultured at 37 °C and 5% CO 2 with the addition of 25 μmol/L of cytochalasin D (Cayman) in the media for 48 h. 10 μg/ml Hoechst 33258 (Invitrogen, H3569), a DNA-binding dye was utilized to stain cardiomyocytes nuclei. ..

    Cell Culture:

    Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure
    Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM HEPES (ThermoFisher Scientific), pH = 7.4, cultured at 37 °C and 5% CO 2 with the addition of 25 μmol/L of cytochalasin D (Cayman) in the media for 48 h. 10 μg/ml Hoechst 33258 (Invitrogen, H3569), a DNA-binding dye was utilized to stain cardiomyocytes nuclei. ..

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold
    Article Snippet: .. All cell culture media and supplements were purchased from PAN-Biotech, except for non-essential amino acids (NEAA), sodium pyruvate, and (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) solution, which was purchased from ThermoFisher. .. Chemical reagents such as Triton X-100, formaldehyde, ALP buffer and NaCl were obtained from Sigma Aldrich, and HCl was obtained from POCh.



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