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proteolysis solution  (Millipore)


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    Structured Review

    Millipore proteolysis solution
    Proteolysis Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteolysis+solution/proteolysis+solution/pmc02394868-53-62-59
    Average 90 stars, based on 1 article reviews
    proteolysis solution - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    In Situ:

    Article Title: Taxonomic and Strain-Specific Identification of the Probiotic Strain Lactobacillus rhamnosus 35 within the Lactobacillus casei Group
    Article Snippet: .. Cells included in the agarose blocks were lysed in situ with 10 ml of lysis solution (1 mg/ml lysozyme [Sigma-Aldrich, St-Quentin-Fallavier, France], 400 U mutanolysin [Sigma-Aldrich], 6 mM Tris-HCl, 1 M NaCl, 100 mM EDTA [pH 7.6], 0.2% sodium deoxycholate, 1% Na-lauroylsarcosine) at 37°C for 24 h and then with 5 ml of proteolysis solution (1 mg/ml proteinase K [Sigma-Aldrich], 250 mM EDTA [pH 8], 1% Na-lauroylsarcosine, 0.2% sodium deoxycholate) at 50°C for 48 h. The agarose blocks were washed under agitation once at 37°C for 1 h with 20 ml of 1 mM phenylmethylsulfonyl fluoride in 1× TE (10 mM Tris-HCl [pH 8], 1 mM EDTA), once at room temperature for 1 h with 20 ml of 1 mM phenylmethylsulfonyl fluoride in 1× TE, and three times at room temperature for 1 h with 20 ml of 1× TE. ..

    Lysis:

    Article Title: Taxonomic and Strain-Specific Identification of the Probiotic Strain Lactobacillus rhamnosus 35 within the Lactobacillus casei Group
    Article Snippet: .. Cells included in the agarose blocks were lysed in situ with 10 ml of lysis solution (1 mg/ml lysozyme [Sigma-Aldrich, St-Quentin-Fallavier, France], 400 U mutanolysin [Sigma-Aldrich], 6 mM Tris-HCl, 1 M NaCl, 100 mM EDTA [pH 7.6], 0.2% sodium deoxycholate, 1% Na-lauroylsarcosine) at 37°C for 24 h and then with 5 ml of proteolysis solution (1 mg/ml proteinase K [Sigma-Aldrich], 250 mM EDTA [pH 8], 1% Na-lauroylsarcosine, 0.2% sodium deoxycholate) at 50°C for 48 h. The agarose blocks were washed under agitation once at 37°C for 1 h with 20 ml of 1 mM phenylmethylsulfonyl fluoride in 1× TE (10 mM Tris-HCl [pH 8], 1 mM EDTA), once at room temperature for 1 h with 20 ml of 1 mM phenylmethylsulfonyl fluoride in 1× TE, and three times at room temperature for 1 h with 20 ml of 1× TE. ..



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