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protein digestion solution  (MedChemExpress)


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    Structured Review

    MedChemExpress protein digestion solution
    Protein Digestion Solution, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteolysis+solution/Protein+A/pm38788831-80-12-34
    Average 95 stars, based on 35 article reviews
    protein digestion solution - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: SERPINI1 functions as a tumor suppressor in glioma.
    Article Snippet: For Co-IP assays, clarified cell lysates from T98G cells were incubated overnight at 4 °C with gentle rotation with either an anti-SERPINI1 antibody (2 μg; Proteintech, China) or control IgG (2 μg; Byotime, China). .. Subsequently, 30 μL of Protein A Sepharose bead (MCE, China) slurry was added to each sample, followed by incubation for an additional 2 hours at 4°C to capture the antibody-protein complexes. ..

    Article Title: A tRNA-derived fragment tRF3019a promotes LUAD metastasis by stabilizing hnRNPK and enhancing MYH11 expression
    Article Snippet: Non-denaturing cell lysates were prepared using Western and IP Lysis Buffer (Beyotime) supplemented with 1× protease inhibitor cocktail without EDTA (MedChemExpress). .. The supernatants were incubated overnight with the indicated antibodies (2 μg of antibody per 500 μg of protein) and Protein A + G magnetic beads (MedChemExpress) for 3 h at 4 °C. .. The antibodies used for immunoprecipitation were: hnRNPK (ab52600, Abcam), anti-His-tag (ab213204, Abcam), hnRNPK (ab23644, Abcam), and FBXO4 (MA5-21,639, Thermo Scientific).

    Article Title: Fat mass and obesity–mediated N 6 -methyladenosine modification modulates neuroinflammatory responses after traumatic brain injury
    Article Snippet: .. To evaluate protein stability, the BV2 cells were incubated with 30 μg/mL cycloheximide (MedChemExpress) for 0, 2, 4, 6 and 8 hours and harvested, followed by western blotting detection of ADAM17. ..

    Article Title: Huanglian muxiang decoction alleviates FTH–mediated ferroptosis in colitis by inhibiting the PI3K–AKT pathway
    Article Snippet: Background: Dysregulated iron metabolism and subsequent ferroptosis play a critical role in the pathogenesis of ulcerative colitis (UC), highlighting iron homeostasis as a therapeutic target.. Huanglian muxiang decoction (HMD), a traditional Chinese herbal formula, is widely utilized for gastrointestinal disorders such as colitis.. However, its underlying molecular mechanisms remain unclear.

    Magnetic Beads:

    Article Title: A tRNA-derived fragment tRF3019a promotes LUAD metastasis by stabilizing hnRNPK and enhancing MYH11 expression
    Article Snippet: Non-denaturing cell lysates were prepared using Western and IP Lysis Buffer (Beyotime) supplemented with 1× protease inhibitor cocktail without EDTA (MedChemExpress). .. The supernatants were incubated overnight with the indicated antibodies (2 μg of antibody per 500 μg of protein) and Protein A + G magnetic beads (MedChemExpress) for 3 h at 4 °C. .. The antibodies used for immunoprecipitation were: hnRNPK (ab52600, Abcam), anti-His-tag (ab213204, Abcam), hnRNPK (ab23644, Abcam), and FBXO4 (MA5-21,639, Thermo Scientific).

    other:

    Article Title: Ochratoxin A drives hepatocellular carcinoma progression via GRPR-mediated activation of PLCβ/IP3R/Ca²⁺ and RPS15A/p53 pathways by regulating cancer cell fate.
    Article Snippet: Protein bands were visualized using a Western Lightning Plus-ECL kit (Syngene, Cambridge, UK), and band intensities were quantified with the ImageJ software relative to β-actin (Zhang et al., 2023).

    Article Title: Uridine metabolism promotes lung adenocarcinoma progression by activating FBL transcription via YBX1.
    Article Snippet: Protein degradation assay was performed by treating cells with CHX (MedChemExpress, HY-12320, 20 μg/ml) at indicated time points, then cells were lysed and followed by immunoblotting.

    Stability:

    Article Title: Fat mass and obesity–mediated N 6 -methyladenosine modification modulates neuroinflammatory responses after traumatic brain injury
    Article Snippet: .. To evaluate protein stability, the BV2 cells were incubated with 30 μg/mL cycloheximide (MedChemExpress) for 0, 2, 4, 6 and 8 hours and harvested, followed by western blotting detection of ADAM17. ..

    Western Blot:

    Article Title: Fat mass and obesity–mediated N 6 -methyladenosine modification modulates neuroinflammatory responses after traumatic brain injury
    Article Snippet: .. To evaluate protein stability, the BV2 cells were incubated with 30 μg/mL cycloheximide (MedChemExpress) for 0, 2, 4, 6 and 8 hours and harvested, followed by western blotting detection of ADAM17. ..

    Stability Assay:

    Article Title: FBXO39 promotes LDHA-mediated aerobic glycolysis and colorectal cancer progression by p53 degradation
    Article Snippet: Colony counting was performed using ImageJ (v1.8). .. For the protein stability assay, cells stably transduced with lentivirus were treated with 25 μg/ml cycloheximide (CHX; HY12320, MedChemExpress) for specified time periods. .. After treatment, cells were harvested and lysed using cell lysis buffer (87787, Thermo Fisher) supplemented with both protease and phosphatase inhibitors (78442, Thermo Fisher).

    Article Title: FBXO39 promotes LDHA-mediated aerobic glycolysis and colorectal cancer progression by p53 degradation.
    Article Snippet: .. For the protein stability assay, cells stably transduced with lentivirus were treated with 25 μg/ml cycloheximide (CHX; HY12320, MedChemExpress) for specified time periods. .. After treatment, cells were harvested and lysed using cell lysis buffer (87787, Thermo Fisher) supplemented with both protease and phosphatase inhibitors (78442, Thermo Fisher).

    Stable Transfection:

    Article Title: FBXO39 promotes LDHA-mediated aerobic glycolysis and colorectal cancer progression by p53 degradation
    Article Snippet: Colony counting was performed using ImageJ (v1.8). .. For the protein stability assay, cells stably transduced with lentivirus were treated with 25 μg/ml cycloheximide (CHX; HY12320, MedChemExpress) for specified time periods. .. After treatment, cells were harvested and lysed using cell lysis buffer (87787, Thermo Fisher) supplemented with both protease and phosphatase inhibitors (78442, Thermo Fisher).

    Article Title: FBXO39 promotes LDHA-mediated aerobic glycolysis and colorectal cancer progression by p53 degradation.
    Article Snippet: .. For the protein stability assay, cells stably transduced with lentivirus were treated with 25 μg/ml cycloheximide (CHX; HY12320, MedChemExpress) for specified time periods. .. After treatment, cells were harvested and lysed using cell lysis buffer (87787, Thermo Fisher) supplemented with both protease and phosphatase inhibitors (78442, Thermo Fisher).

    Transduction:

    Article Title: FBXO39 promotes LDHA-mediated aerobic glycolysis and colorectal cancer progression by p53 degradation
    Article Snippet: Colony counting was performed using ImageJ (v1.8). .. For the protein stability assay, cells stably transduced with lentivirus were treated with 25 μg/ml cycloheximide (CHX; HY12320, MedChemExpress) for specified time periods. .. After treatment, cells were harvested and lysed using cell lysis buffer (87787, Thermo Fisher) supplemented with both protease and phosphatase inhibitors (78442, Thermo Fisher).

    Article Title: FBXO39 promotes LDHA-mediated aerobic glycolysis and colorectal cancer progression by p53 degradation.
    Article Snippet: .. For the protein stability assay, cells stably transduced with lentivirus were treated with 25 μg/ml cycloheximide (CHX; HY12320, MedChemExpress) for specified time periods. .. After treatment, cells were harvested and lysed using cell lysis buffer (87787, Thermo Fisher) supplemented with both protease and phosphatase inhibitors (78442, Thermo Fisher).



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