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cm10 proteinchip arrays  (Bio-Rad)


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    Structured Review

    Bio-Rad cm10 proteinchip arrays
    Cm10 Proteinchip Arrays, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteinchip+array+cm10/ProteinChip+CM10+Arrays/pmc05472376-76-0-3
    Average 93 stars, based on 86 article reviews
    cm10 proteinchip arrays - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: Isolation and Proteomic Analysis of Platelets by SELDI-TOF MS
    Article Snippet: We highly recommend that all binding buffers also be purchased from Bio-Rad to avoid any quality control issues that may arise from researchers preparing their own buffers.

    Article Title: Investigation of serum protein profiles in scrapie infected sheep by means of SELDI-TOF-MS and multivariate data analysis
    Article Snippet: A Weak cation exchange array (ProteinChip® CM10 Array, Bio-Rad) in combination with high stringency buffer, 50 mM HEPES pH 7.0 as binding and washing buffer was used to analyse the flow through (FT) fraction in this work.

    Article Title: Clinical decision support (CDS) for radiotherapy in prostate cancer
    Article Snippet: The chromatographic chips were part of BIORAD PROTEINCHIP CM10 ARRAYS.

    Article Title: Putative salivary biomarkers useful to differentiate patients with fibromyalgia.
    Article Snippet: Please cite this article as: Federica Ciregia, Camillo Giacomelli, Laura Giusti, Claudia Boldrini, Isabella Piga, Pasquale Pepe, Arianna Consensi, Sara Gori, Antonio Lucacchini, Maria R. Mazzoni, Laura Bazzichi , Putative salivary biomarkers useful to differentiate patients with fibromyalgia.. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Isolation and Proteomic Analysis of Platelets by SELDI-TOF MS
    Article Snippet: CM10 ProteinChip Arrays (Bio-Rad).

    Article Title: Isolation and Proteomic Analysis of Platelets by SELDI-TOF MS
    Article Snippet: There are other chemistries available such as Immobilized Metal Affinity Capture (IMAC30) and Hydrophobic (H50) and the corresponding buffers, available from Bio-Rad, should be used in conjunction with the other chemistries. list-behavior=enumeratedprefix-word=mark-type=decimalmax-label-size=0 Equilibrate CM10 ProteinChip Arrays with 150 μL CM10 Low Stringency Buffer for 5 min with vigorous shaking at room temperature.

    Binding Assay:

    Article Title: A Fragment of the LG3 Peptide of Endorepellin Is Present in the Urine of Physically Active Mining Workers: A Potential Marker of Physical Activity
    Article Snippet: The protein concentration of each urinary protein sample was determined in triplicate by bicinchoninic acid (BCA) protein assay (Pierce, Rockford, IL, USA) as per the manufacturer's instructions and adjusted to 0.42 mg/mL in binding buffer (10 mM sodium acetate (NaAc), pH 4.5). .. CM10 (weak cation exchange) ProteinChip arrays (Bio-Rad, Hercules, CA, USA) were then pre-equilibrated twice with 50-μl of binding buffer using a ProteinChip Cassette – Compatible Bioprocessor (Cat # C50-30011) (BioRad) and placed on a plate shaker for 5 min. Triplicate 35 μL sub-samples of urinary protein and a single QC replicate sample were applied to the surface of the CM10 arrays in a semi-randomised fashion to ensure that no replicate samples occupied a second spot on individual arrays. ..

    QC/EQA/Proficiency:

    Article Title: A Fragment of the LG3 Peptide of Endorepellin Is Present in the Urine of Physically Active Mining Workers: A Potential Marker of Physical Activity
    Article Snippet: The protein concentration of each urinary protein sample was determined in triplicate by bicinchoninic acid (BCA) protein assay (Pierce, Rockford, IL, USA) as per the manufacturer's instructions and adjusted to 0.42 mg/mL in binding buffer (10 mM sodium acetate (NaAc), pH 4.5). .. CM10 (weak cation exchange) ProteinChip arrays (Bio-Rad, Hercules, CA, USA) were then pre-equilibrated twice with 50-μl of binding buffer using a ProteinChip Cassette – Compatible Bioprocessor (Cat # C50-30011) (BioRad) and placed on a plate shaker for 5 min. Triplicate 35 μL sub-samples of urinary protein and a single QC replicate sample were applied to the surface of the CM10 arrays in a semi-randomised fashion to ensure that no replicate samples occupied a second spot on individual arrays. ..

    Mass Spectrometry:

    Article Title: Functionalized cerium oxide nanoparticles mitigate the oxidative stress and pro-inflammatory activity associated to the portal vein endothelium of cirrhotic rats
    Article Snippet: .. Protein profiling was performed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) using the eight-spot format CM10 ProteinChip arrays (Bio-Rad, Hercules, CA, USA). ..



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    Image Search Results


    Classification results

    Journal: Proteome Science

    Article Title: A critical assessment of SELDI-TOF-MS for biomarker discovery in serum and tissue of patients with an ovarian mass

    doi: 10.1186/1477-5956-10-45

    Figure Lengend Snippet: Classification results

    Article Snippet: Protein profiles of serum and tissue samples were generated using anionic surfaces of CM10 and cationic surfaces of Q10 ProteinChip arrays (Ciphergen Biosystems Inc., Fremont, CA.).

    Techniques:

    Pairwise comparisons between tumors of low malignant potential and hierarchical clustering of tissue data. Table with pairwise comparisons between mucinous and serous tumors of low malignant potential, cancer and benign tumor tissue. The number of upregulated (+) and downregulated (−) peaks is given for each comparison. Hierarchical clustering of CM10 tissue data. Clustering of 84 tissue samples using complete linkage and Pearson correlation distance on the 62 peaks that were differentially expressed (adjusted p-value < 0.05) between cancer, low malignant potential (divided in mucinous and serous), and benign tumor. The Z-score is calculated on the columns by subtracting the mean expression value of a column from each of the values and then dividing the resulting values by the standard deviation of the column. Color in the heat maps, therefore, indicates the relative expression level, with red being higher and blue lower than the mean expression value. The vertical side bar indicates the sample type: benign (red), mucinous LMP (green), serous LMP (purple), cancer (blue).

    Journal: Proteome Science

    Article Title: A critical assessment of SELDI-TOF-MS for biomarker discovery in serum and tissue of patients with an ovarian mass

    doi: 10.1186/1477-5956-10-45

    Figure Lengend Snippet: Pairwise comparisons between tumors of low malignant potential and hierarchical clustering of tissue data. Table with pairwise comparisons between mucinous and serous tumors of low malignant potential, cancer and benign tumor tissue. The number of upregulated (+) and downregulated (−) peaks is given for each comparison. Hierarchical clustering of CM10 tissue data. Clustering of 84 tissue samples using complete linkage and Pearson correlation distance on the 62 peaks that were differentially expressed (adjusted p-value < 0.05) between cancer, low malignant potential (divided in mucinous and serous), and benign tumor. The Z-score is calculated on the columns by subtracting the mean expression value of a column from each of the values and then dividing the resulting values by the standard deviation of the column. Color in the heat maps, therefore, indicates the relative expression level, with red being higher and blue lower than the mean expression value. The vertical side bar indicates the sample type: benign (red), mucinous LMP (green), serous LMP (purple), cancer (blue).

    Article Snippet: Protein profiles of serum and tissue samples were generated using anionic surfaces of CM10 and cationic surfaces of Q10 ProteinChip arrays (Ciphergen Biosystems Inc., Fremont, CA.).

    Techniques: Expressing, Standard Deviation

    Comparison of detected peaks in serum and tissue

    Journal: Proteome Science

    Article Title: A critical assessment of SELDI-TOF-MS for biomarker discovery in serum and tissue of patients with an ovarian mass

    doi: 10.1186/1477-5956-10-45

    Figure Lengend Snippet: Comparison of detected peaks in serum and tissue

    Article Snippet: Protein profiles of serum and tissue samples were generated using anionic surfaces of CM10 and cationic surfaces of Q10 ProteinChip arrays (Ciphergen Biosystems Inc., Fremont, CA.).

    Techniques: