nsp7 97 096 (ProSci Incorporated)
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nsp7 97 096
Nsp7 97 096, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+structures/SARS-CoV-2+(COVID-19)+NSP7+Recombinant+Protein/pmc08577385-152-5-14
Average 93 stars, based on 2 article reviews
Nsp7 97 096, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+structures/SARS-CoV-2+(COVID-19)+NSP7+Recombinant+Protein/pmc08577385-152-5-14
Average 93 stars, based on 2 article reviews
nsp7 97 096 - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin"
Article Title: Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin
Journal: Journal of Virology
doi: 10.1128/JVI.01396-21
Figure Legend Snippet: SARS-CoV2 nucleocapsid protein (NP) is a potent inducer of human endothelial cell activation. (a) HLMECs were incubated with SARS-CoV2 structural proteins (S, N, and E proteins; 1 μg/ml) and five nonstructural proteins (NSP1, NSP3, NSP5, NSP7, and NSP8; 1 μg/ml) for 8 h. (b) HLMECs were treated with 1 μg/ml of NP or 10 ng/ml of TNF-α for different incubation periods as indicated. (c) HLMECs were incubated with indicated concentrations of NP for 8 h. TNF-α at 10 ng/ml served as a positive control. (d) Different cultured cells, including mouse lung vascular endothelial cells (MECs), A549 cells, 293T cells, HUVECs, HAECs, HCAECs, HDMECs, and HLMECs, were treated with NP (1 μg/ml) for 8 h. The expression of ICAM-1, VCAM-1, and VE-cadherin was detected by Western blotting. β-Actin served as a loading control. (e) HLMECs were treated with PBS, NP (1 μg/ml), TNF-α (10 ng/ml), or lipopolysaccharide (LPS) (1 μg/ml) for 8 h. The total RNA was isolated and qPCR was performed for measuring the mRNA levels of TNF-α, ICAM-1, VCAM-1, MCP-1, and IL-6. (f) HLMECs were treated with PBS, NP (1 μg/ml), or TNF-α (10 ng/ml) for 8 h and cocultured with Zombie Red-labeled THP-1 cells for 1 h. After being washed, the adherent cells were imaged and quantitatively analyzed.
Techniques Used: Activation Assay, Incubation, Positive Control, Cell Culture, Expressing, Western Blot, Control, Isolation, Labeling
Related Articles
Activation Assay:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Incubation:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Positive Control:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Cell Culture:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Expressing:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Western Blot:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Control:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Isolation:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). Labeling:Article Title: SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Article Snippet: SARSCoV-2 NSP1 (97-095), NSP5 (10-116), NSP7 (97-096), and Article Title: Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin Article Snippet: SARS-CoV-2 nucleocapsid protein (NUN-C5227), S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). |
