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omni easytm protein sample loading buffer  (Epizyme Inc)

 
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    Epizyme Inc omni easytm protein sample loading buffer
    Omni Easytm Protein Sample Loading Buffer, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+loading+buffer/easytm+omni/pm42265110-389-23-31
    Average 86 stars, based on 1 article reviews
    omni easytm protein sample loading buffer - by Bioz Stars, 2026-11
    86/100 stars

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    SDS Page:

    Article Title: Bushen Yijing formula exerts chondroprotective effects in knee osteoarthritis via the ERα/PI3K/AKT signaling pathway: Insights from network pharmacology and experimental validation.
    Article Snippet: Total protein concentration was assessed with the BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Equal amounts of protein were separated by SDS-PAGE using gels prepared with the Omni-EasyTM One-Step Color PAGE Gel Rapid Preparation Kit (Epizyme Biotech, PG212). .. The total proteins were transferred to a PVDF membrane (Merck Millipore, IPVH00010) and blocked with Rapid Blocking Solution (Epizyme Biotech, PS108P) to minimize non-specific binding.

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Article Title: Grass carp catalase inhibits GCRV replication through ROS scavenging-mediated immune suppression and autophagy.
    Article Snippet: .. Protein samples were prepared by adding Omni-EasyTM 5× SDS-PAGE Loading Buffer (EpiZyme, Cat# LT101), mixing thoroughly, and denaturing at 100°C for 10 minutes. .. Proteins were separated on 4-20% gradient SDS-PAGE gels (GenScript, Cat# M01004) and subsequently transferred onto PVDF membranes (Millipore, Cat# 3010040001).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Bushen Yijing formula exerts chondroprotective effects in knee osteoarthritis via the ERα/PI3K/AKT signaling pathway: Insights from network pharmacology and experimental validation.
    Article Snippet: Total protein concentration was assessed with the BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Equal amounts of protein were separated by SDS-PAGE using gels prepared with the Omni-EasyTM One-Step Color PAGE Gel Rapid Preparation Kit (Epizyme Biotech, PG212). .. The total proteins were transferred to a PVDF membrane (Merck Millipore, IPVH00010) and blocked with Rapid Blocking Solution (Epizyme Biotech, PS108P) to minimize non-specific binding.

    Article Title: CLPX acquires an iron-sulfur cluster to sustain mitochondrial proteostasis in cancer cells.
    Article Snippet: Cell samples or tumor tissue specimens were washed in pre-cooled PBS and lysed in RIPA Lysis buffer (PC101, Epizyme) containing protease inhibitor phenylsulfonyl fluoride (PMSF, T0789, Target Molecule Corporation). .. The protein concentration of the lysates was quantified by the Protein Quantification Kit (KTD3001, Abbkine Scientific), after which the samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with gels prepared using the Omni-EasyTM One-step Color PAGE Gel Rapid Preparation Kit (PG210, Epizyme). .. The separated proteins were then electrotransferred onto polyvinylidene fluoride (PVDF) membranes (0.22μm, WJ002S, Epizyme).

    Article Title: Hyperactivated glycolysis drives spatially patterned Kupffer cell depletion in MASLD
    Article Snippet: Chemical compound, drug , Antifade Mounting Medium with DAPI , Beyotime , Cat# P0131 , . .. Chemical compound, drug , Omni-Easy One-Step PAGE Gel Fast Preparation Kit , Epizyme , Cat# PG213 , . .. Chemical compound, drug , SDS , BBI , Cat# A600485-0500 , .

    Article Title: ALA-PDT induces apoptosis in HPV-transformed cells through mtDNA release-mediated activation of the cGAS-STING pathway.
    Article Snippet: .. Samples were denatured at 100 ◦ C for 10 min in a metal bath, cooled to room temperature, and aliquoted for long-term storage at –80 ◦ C. Gels were prepared using the Omni-EasyTM One-Step PAGE Gel Fast Preparation Kit (10%) (EpiZyme, China) in BIO-RAD mini gel systems. .. After electrophoresis, membrane transfer, and blocking, membranes were incubated with antibodies and detected by chemiluminescence using a Tanon imaging system (Tanon, China).

    Protein Concentration:

    Article Title: CLPX acquires an iron-sulfur cluster to sustain mitochondrial proteostasis in cancer cells.
    Article Snippet: Cell samples or tumor tissue specimens were washed in pre-cooled PBS and lysed in RIPA Lysis buffer (PC101, Epizyme) containing protease inhibitor phenylsulfonyl fluoride (PMSF, T0789, Target Molecule Corporation). .. The protein concentration of the lysates was quantified by the Protein Quantification Kit (KTD3001, Abbkine Scientific), after which the samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with gels prepared using the Omni-EasyTM One-step Color PAGE Gel Rapid Preparation Kit (PG210, Epizyme). .. The separated proteins were then electrotransferred onto polyvinylidene fluoride (PVDF) membranes (0.22μm, WJ002S, Epizyme).

    other:

    Article Title: Nitazoxanide cooperates with cytarabine to inhibit cytarabine-resistant acute myeloid leukemia progression via mitochondrial dysfunction and PLK1 suppression.
    Article Snippet: Acute myeloid leukemia (AML) remains a therapeutic challenge due to frequent resistance to cytarabine (Ara-C), the standard chemotherapeutic agent.. Recent studies implicate mitochondrial oxidative phosphorylation (OXPHOS) as a pivotal driver of this resistance, conferring metabolic adaptability and survival advantages to leukemic cells.. In this study, we evaluated the therapeutic potential of repurposing nitazoxanide (NTZ), an FDAapproved antiparasitic drug, to to overcome Ara-C resistance in AML.

    Cell Counting:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Staining:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Article Title: CLPX acquires an iron-sulfur cluster to sustain mitochondrial proteostasis in cancer cells.
    Article Snippet: .. For Coomassie blue staining, the insoluble fraction was resuspended in pre-cooled RIPA Lysis buffer containing 1mM PMSF, mixed with 1/4 volume of 5× Omni-EasyTM Protein Sample Loading Buffer (Denaturing, Reducing, LT101, Epizyme), separated by SDS-PAGE, and visualized byCoomassie Blue Staining Solution (P0017B, Beyotime Biotechnology). ..

    Recombinant:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Activity Assay:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Calcium Colorimetric Assay:

    Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
    Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.

    Lysis:

    Article Title: CLPX acquires an iron-sulfur cluster to sustain mitochondrial proteostasis in cancer cells.
    Article Snippet: .. For Coomassie blue staining, the insoluble fraction was resuspended in pre-cooled RIPA Lysis buffer containing 1mM PMSF, mixed with 1/4 volume of 5× Omni-EasyTM Protein Sample Loading Buffer (Denaturing, Reducing, LT101, Epizyme), separated by SDS-PAGE, and visualized byCoomassie Blue Staining Solution (P0017B, Beyotime Biotechnology). ..



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