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Structured Review

Valiant Co Ltd bn loading dye
Bn Loading Dye, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+loading+buffer/Protein+Loading+Buffer+(SDS-PAGE)/pm38570685-592-21-29
Average 94 stars, based on 5 article reviews
bn loading dye - by Bioz Stars, 2026-10
94/100 stars

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Related Articles

Centrifugation:

Article Title: Pharmacological Inhibition of JAK/STAT-IL2 Axis Alleviated Cisplatin-Induced Ototoxicity.
Article Snippet: Sensorineural hearing loss (SNHL) is recognized as one of the most common sensory disorders and is characterized by irreversible damage to cochlear hair cells (HCs).. Our research, which utilized the HEI-OC1 cell line for drug screening, revealed that inhibiting the JAK/STAT signaling pathway protects HEI-OC1 cells from cisplatin-induced ototoxicity.. Further studies with cochlear explants showed that ifidancitinib, an inhibitor of both JAK1 and JAK3, offered superior protection compared with other JAK inhibitors.

Protein Concentration:

Article Title: Ancestral allele of DNA polymerase gamma modifies antiviral tolerance.
Article Snippet: .. The clarified supernatants were measured for protein concentration using the BCA assay and equal amounts of protein samples were mixed with BN loading dye (0.25% (w/v) Coomassie blue G250 (MP Biomedicals), 75 mM α-amino n-caproic acid) before BN electrophoresis using cathode buffer (50 mM tricine, 15 mM Bis-Tris, pH 7.0, 0.02% (w/v) Coomassie blue G250) and anode buffer (50 mM Bis-Tris, pH 7.0) on self-casted 1-mm-thick 5–12% gradient polyacrylamide gels. ..

BIA-KA:

Article Title: Ancestral allele of DNA polymerase gamma modifies antiviral tolerance.
Article Snippet: .. The clarified supernatants were measured for protein concentration using the BCA assay and equal amounts of protein samples were mixed with BN loading dye (0.25% (w/v) Coomassie blue G250 (MP Biomedicals), 75 mM α-amino n-caproic acid) before BN electrophoresis using cathode buffer (50 mM tricine, 15 mM Bis-Tris, pH 7.0, 0.02% (w/v) Coomassie blue G250) and anode buffer (50 mM Bis-Tris, pH 7.0) on self-casted 1-mm-thick 5–12% gradient polyacrylamide gels. ..

Electrophoresis:

Article Title: Ancestral allele of DNA polymerase gamma modifies antiviral tolerance.
Article Snippet: .. The clarified supernatants were measured for protein concentration using the BCA assay and equal amounts of protein samples were mixed with BN loading dye (0.25% (w/v) Coomassie blue G250 (MP Biomedicals), 75 mM α-amino n-caproic acid) before BN electrophoresis using cathode buffer (50 mM tricine, 15 mM Bis-Tris, pH 7.0, 0.02% (w/v) Coomassie blue G250) and anode buffer (50 mM Bis-Tris, pH 7.0) on self-casted 1-mm-thick 5–12% gradient polyacrylamide gels. ..

Nucleic Acid Electrophoresis:

Article Title: MLL1 downregulation drives hair cell ferroptosis via mitochondrial and endoplasmic reticulum stress mechanisms through PERK–eIF2α–Atf4–Chop and PI3K/Akt–Lrp1 signaling pathway
Article Snippet: Protein extracts were obtained from HEI-OC1 cells for each group using radioimmunoprecipitation assay (RIPA) buffer (P0013B; Beyotime Biotechnology) containing a protease inhibitor (04693132001; Sigma-Aldrich). .. Then, the lysates were kept on ice for 30 min while being shaken every 10 min. After being centrifuged at 12,000 revolutions per minute (r = 8 cm) and 4°C for 10 min, the resulting supernatants were treated with protein loading buffer (08W00015; MP Biomedicals, Santa Ana, CA, USA) at 95°C for denaturation and were then size-separated by 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). .. Proteins were subsequently transferred to polyvinylidene difluoride (PVDF) membranes (IPVH00010; Merck Millipore, Zug, Switzerland) in an ice bath.

SDS Page:

Article Title: Manganese porphyrin reduces retinal injury induced by ocular hypertension in rats.
Article Snippet: Horseradish peroxidaseeconjugated goat anti-rabbit IgG (1:10,000 dilution; Zymed Laboratories, San Francisco, CA) was used as a secondary antibody, and immunoreactive proteins were visualized by chemiluminescence via ECL reagent (Amersham Pharmacia Biotech, Buckinghamshire, England). .. Proteins separated by SDS-PAGE were also visualized by Electro-Blue Staining solution (Qbiogene, Heidelberg, Germany) to confirm equal protein loading in each lane (Cort et al., 2010). .. Samples were transferred to an ultrafiltration unit and centrifuged through a 10 kDa molecular mass cut-off filter (Centricon, Millipore Corporation) for 1 h to remove protein.

Staining:

Article Title: Manganese porphyrin reduces retinal injury induced by ocular hypertension in rats.
Article Snippet: Horseradish peroxidaseeconjugated goat anti-rabbit IgG (1:10,000 dilution; Zymed Laboratories, San Francisco, CA) was used as a secondary antibody, and immunoreactive proteins were visualized by chemiluminescence via ECL reagent (Amersham Pharmacia Biotech, Buckinghamshire, England). .. Proteins separated by SDS-PAGE were also visualized by Electro-Blue Staining solution (Qbiogene, Heidelberg, Germany) to confirm equal protein loading in each lane (Cort et al., 2010). .. Samples were transferred to an ultrafiltration unit and centrifuged through a 10 kDa molecular mass cut-off filter (Centricon, Millipore Corporation) for 1 h to remove protein.



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Image Search Results


Journal: Frontiers in Oncology

Article Title: Integrating network pharmacology, molecular docking, and experimental validation, this study explores the mechanism of action of Typhonii Rhizoma in colon cancer

doi: 10.3389/fonc.2026.1815506

Figure Lengend Snippet: Experiment-related reagents.

Article Snippet: 5× Protein Loading Buffer (DTT-containing) , Epizyme , 4% Hematoxylin and Eosin Fixative , Solarbio.

Techniques: CCK-8 Assay, Protease Inhibitor, Western Blot, Membrane, Electrophoresis