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geci genetically encoded indicators of neuronal activity  (Nagai Nori USA INC)

 
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    Structured Review

    Nagai Nori USA INC geci genetically encoded indicators of neuronal activity
    Geci Genetically Encoded Indicators Of Neuronal Activity, supplied by Nagai Nori USA INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+function/functional+fluorescent+ca2++indicator+proteins/pmc07969681-78-15-12
    Average 90 stars, based on 1 article reviews
    geci genetically encoded indicators of neuronal activity - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Imaging:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Expressing:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Functional Assay:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Transgenic Assay:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Control:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Concentration Assay:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    In Vitro:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Binding Assay:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Fluorescence:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    In Vivo:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.

    Activity Assay:

    Article Title: Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging.
    Article Snippet: 570 Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, 571 Griesbeck O, Tsien RY, Nagai T, Miyawaki A, Denk W (2004) Functional fluorescent Ca2+ indicator 572 proteins in transgenic mice under TET control.

    Article Title: Photo-transformable genetically-encoded optical probes for functional highlighting in vivo.
    Article Snippet: Studying the brain requires knowledge about both structure (i.e., connectome) and function of its constituents (neurons and glia alike).. This need has prompted the development of novel tools and techniques, in particular optical techniques to examine cells remotely.. Early works (1900’s) led to the development of novel cell-staining techniques that, when combined with the use of a very simple light microscope, visualized individual neurons and their subcellular compartments in fixed tissues.

    Article Title: Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
    Article Snippet: We estimated the Ca2+ concentration in live cells based on the in vitro calibration in this work, and the values were consistent with previous reports on classical genetically encoded Ca2+ probes (Nagai et al., PNAS 2004, Miyawaki et al., PNAS 1999), so we believe this method is reliable enough.

    Article Title: Methods of measuring presynaptic function with fluorescence probes
    Article Snippet: Calcium binding to GECI causes conformational rearrangement and fluorescence (Perez Koldenkova and Nagai ), and GECI makes it easy to observe calcium for a few days in vitro or in vivo by genetic modifications (genetically encoded indicators of neuronal activity).

    Article Title: Reduced Ca 2+ transient amplitudes may signify increased or decreased depolarization depending on the neuromodulatory signaling pathway.
    Article Snippet: Ca2+ imaging is a powerful and widely used approach (Patriarchi et al., 2018; Corkrum et al., 2020; Redolfi et al., 2021) and, thus far, preferred over fluorescent voltage indicators due to superior sensitivity, kinetics, and ease of expression (Vogt, 2015; Inagaki and Nagai, 2016; Mollinedo-Gajate et al., 2019; Inoue, 2021; Zhu et al., 2021).

    Article Title: Mismatch negativity (MMN) as a tool for translational investigations into early psychosis: A review.
    Article Snippet: Several CHR criteria were used such as the Bonn scale for the assessment of basic symptoms (BSABS) (Gross et al., 1987) (MMN studies: BrockhausDumke et al., 2005; Bodatsch et al., 2011), the structured interview for prodromal symptoms (SIPS) (Miller et al., 2002) (MMN studies: Jahshan et al., 2012; Nagai et al., 2013b; Perez et al., 2014; SolísVivanco et al., 2014; Lavoie et al., 2018) or the comprehensive assessment of at-risk mental state (CAARMS) (Yung et al., 2005) (MMN studies: Shin et al., 2009; Atkinson et al., 2012; Hsieh et al., 2012; Shaikh et al., 2012; Higuchi et al., 2013; Higuchi et al., 2014; Atkinson et al., 2017).

    Article Title: Harnessing the new emerging imaging technologies to uncover the role of Ca 2+ signalling in plant nutrient homeostasis.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Increasing crop yields by using eco-friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide.



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