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R&D Systems Hematology protein chips
Protein Chips, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 95/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+chip+array/Proteome+Profiler+Rat+Cytokine+Array+Kit%2C+Panel+A/pm37078751-74-8-14
Average 95 stars, based on 94 article reviews
protein chips - by Bioz Stars, 2026-10
95/100 stars

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Article Title: Doxazosin Alleviates Chronic Orofacial Pain
Article Snippet: Serum Cytokine Detection Serum cytokine levels in male rats were quantified using the Proteome Profiler Rat Cytokine Array Kit, Panel A (Cat. # ARY008; R&D Systems, Minneapolis, MN, USA) that simultaneously detects 29 different cytokines following the manufacturer’s protocol.

Article Title: Hyperbaric oxygen protects against periodontal bone loss by modulating inflammation and bone remodeling via RANKL/OPG expression in ligature-induced periodontitis
Article Snippet: For this study, data interpretation focused on the following targets relevant to periodontitis pathophysiology: CINC-1 (cytokine-induced neutrophil chemoattractant-1 / also known as CXCL1), CINC-2α/β (cytokine-induced neutrophil chemoattractant-2 alpha/beta / also known as CXCL3), sICAM-1 (soluble intercellular adhesion molecule-1), IL-1α (interleukin-1 alpha), IL-1β (interleukin-1 beta), IL-1ra (interleukin-1 receptor antagonist), LIX (lipopolysaccharide-induced CXC chemokine / CXCL5), L-selectin (leukocyte adhesion molecule CD62L), Thymus chemokine (also known as CCL25 or thymus-expressed chemokine), and TIMP-1 (tissue inhibitor of metalloproteinases-1).

Article Title: Doxazosin Alleviates Chronic Orofacial Pain
Article Snippet: Serum cytokine levels in male rats were quantified using the Proteome Profiler Rat Cytokine Array Kit, Panel A (Cat. # ARY008; R&D Systems, Minneapolis, MN, USA) that simultaneously detects 29 different cytokines following the manufacturer’s protocol.

Expressing:

Article Title: Sustained bioactivity of nucleic acid-encoded EPO- and NGF-functionalized conduits enhances regeneration in long-gap peripheral nerve injuries
Article Snippet: .. Total protein was extracted, and cytokine expression was profiled using the Proteome Profiler Rat Cytokine Array Kit (R&D Systems, ARY008). .. Membranes were processed per manufacturer’s instructions, and chemiluminescence signals were detected using a ChemiDoc imaging system (Bio-Rad).

Article Title: Hippocampal glial alterations are associated with Lamin B1 dysregulation and abnormal nuclear morphology in a rat model of fragile X syndrome.
Article Snippet: .. For cytokine expression analysis from peripheral plasma of WT and Fmr1-Δexon 8 rats, a Proteome Profiler Array (Cat. ARY008, Rat Cytokine Array, Panel A; R&D Systems, Minneapolis, MN, USA) was also used. .. Up to 2.5 mL of trunk blood was collected from each animal into 15 mL tubes with EDTA solution (concentration 1:10) (Cat. 15,575–038, Invitrogen, Thermo Fisher Scientific).

Article Title: Hippocampal glial alterations are associated with Lamin B1 dysregulation and abnormal nuclear morphology in a rat model of fragile X syndrome.
Article Snippet: After homogenization, Triton X-100 (Cat. X100, Sigma-Aldrich) was added to a final concentration of 1%, and the tissue lysates were centrifuged at 10,000 ×g for 5min to remove cell debris. .. The protein concentration of the lysates was determined by BCA protein assay (Cat. 23,227, Pierce, Thermo Scientific), and the relative expression of the cytokines was analyzed using the Proteome Profiler Array (Cat. ARY008, Rat Cytokine Array, Panel A; R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol with minor modifications (for cytokine array coordinates see Table 2). ..

Clinical Proteomics:

Article Title: Hippocampal glial alterations are associated with Lamin B1 dysregulation and abnormal nuclear morphology in a rat model of fragile X syndrome.
Article Snippet: .. For cytokine expression analysis from peripheral plasma of WT and Fmr1-Δexon 8 rats, a Proteome Profiler Array (Cat. ARY008, Rat Cytokine Array, Panel A; R&D Systems, Minneapolis, MN, USA) was also used. .. Up to 2.5 mL of trunk blood was collected from each animal into 15 mL tubes with EDTA solution (concentration 1:10) (Cat. 15,575–038, Invitrogen, Thermo Fisher Scientific).

Quantitation Assay:

Article Title: Tumor-Macrophage-Nerve interactions drive neuroinflammation and neuropathic pain in prostate cancer perineural invasion.
Article Snippet: .. Quantitation of sample protein concentration using a total protein assay was done by using a Fluorescence Luminescence Multimode Microplate Reader (Tecan Infinite M200 Pro®, Tecan Group Ltd., Männedorf, Swiss); the samples (400 μg protein) were analysed using the Proteome ProfilerTM Rat Cytokine Array Kit (Cat.#ARY008; R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s protocol (DePeralta et al., 2016; Yazici et al., 2023). ..

Protein Concentration:

Article Title: Tumor-Macrophage-Nerve interactions drive neuroinflammation and neuropathic pain in prostate cancer perineural invasion.
Article Snippet: .. Quantitation of sample protein concentration using a total protein assay was done by using a Fluorescence Luminescence Multimode Microplate Reader (Tecan Infinite M200 Pro®, Tecan Group Ltd., Männedorf, Swiss); the samples (400 μg protein) were analysed using the Proteome ProfilerTM Rat Cytokine Array Kit (Cat.#ARY008; R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s protocol (DePeralta et al., 2016; Yazici et al., 2023). ..

Article Title: Hippocampal glial alterations are associated with Lamin B1 dysregulation and abnormal nuclear morphology in a rat model of fragile X syndrome.
Article Snippet: After homogenization, Triton X-100 (Cat. X100, Sigma-Aldrich) was added to a final concentration of 1%, and the tissue lysates were centrifuged at 10,000 ×g for 5min to remove cell debris. .. The protein concentration of the lysates was determined by BCA protein assay (Cat. 23,227, Pierce, Thermo Scientific), and the relative expression of the cytokines was analyzed using the Proteome Profiler Array (Cat. ARY008, Rat Cytokine Array, Panel A; R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol with minor modifications (for cytokine array coordinates see Table 2). ..

Fluorescence:

Article Title: Tumor-Macrophage-Nerve interactions drive neuroinflammation and neuropathic pain in prostate cancer perineural invasion.
Article Snippet: .. Quantitation of sample protein concentration using a total protein assay was done by using a Fluorescence Luminescence Multimode Microplate Reader (Tecan Infinite M200 Pro®, Tecan Group Ltd., Männedorf, Swiss); the samples (400 μg protein) were analysed using the Proteome ProfilerTM Rat Cytokine Array Kit (Cat.#ARY008; R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s protocol (DePeralta et al., 2016; Yazici et al., 2023). ..

Bicinchoninic Acid Protein Assay:

Article Title: Hippocampal glial alterations are associated with Lamin B1 dysregulation and abnormal nuclear morphology in a rat model of fragile X syndrome.
Article Snippet: After homogenization, Triton X-100 (Cat. X100, Sigma-Aldrich) was added to a final concentration of 1%, and the tissue lysates were centrifuged at 10,000 ×g for 5min to remove cell debris. .. The protein concentration of the lysates was determined by BCA protein assay (Cat. 23,227, Pierce, Thermo Scientific), and the relative expression of the cytokines was analyzed using the Proteome Profiler Array (Cat. ARY008, Rat Cytokine Array, Panel A; R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol with minor modifications (for cytokine array coordinates see Table 2). ..

Membrane:

Article Title: Region-Dependent Responses to Oxygen–Glucose Deprivation and Melatonin in Neonatal Brain Organotypic Slices
Article Snippet: .. The proteome profile of supernatants was assessed using a membrane-based sandwich immunoassay (Proteome Profiler Rat Cytokine Array Kit, R&D Systems, Minneapolis, MN, USA; Cat. No. ARY008). ..



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Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization <t>time-of-flight</t> <t>(SELDI-TOF)</t> mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.
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Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.

Journal: Breast Cancer Research : BCR

Article Title: Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer

doi: 10.1186/bcr3676

Figure Lengend Snippet: Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.

Article Snippet: All serum samples were initially denatured in buffer containing 8 M urea, 1% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfate) and analyzed by TOF MS on SELDI protein chip arrays (Bio-Rad, Hercules, CA, USA) as previously described [ ].

Techniques: Western Blot, Mass Spectrometry, Standard Deviation, Derivative Assay