rapid blocking solution (Beijing Solarbio Science)

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Beijing Solarbio Science rapid blocking solution
Rapid Blocking Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+block+solution/protein+free+rapid+blocking+solution/pmc12002075-99-7-10
Average 90 stars, based on 1 article reviews

rapid blocking solution - by Bioz Stars, 2026-11

90/100 stars

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Membrane:

Article Title: Acupotomy Ameliorates KOA Related Chondrocyte Premature Senescence Through YAP/FOXD1 Pathway
Article Snippet: A 12% SDS-PAGE (Nanjing Aoqing Bioengineering Institute, Nanjing, China) was used to load 3 μL of marker and 10 μL of protein samples at 160 V for 30 min. Proteins were then transferred to a 0.22 μm polyvinylidene difluoride (PVDF) membrane (Millipore, Boston, Mass, USA) at 400 mA for 25 minutes. .. The PVDF membrane was placed in a rapid blocking solution (Solarbio, Wuhan, China) at room temperature for 20 minutes, followed by overnight incubation at 4°C with primary antibodies against anti-P-YAP (1:1000, Ser127, Affbiotech, Cincinnati, OH, USA), YAP (1:1000, Bioss, Beijing, China), FOXD1 (1:2000, Bioss, Beijing, China), Rb1 (1:2000, https://doi.org/10.2147/JPR.S475829 Journal of Pain Research 2025:18 2014 Powered by TCPDF (www.tcpdf.org) Bioss, Beijing, China), p53 (1:1000, Proteintech, Chicago, United States) and GAPDH (1:10,000, Abcam, Cambridge, UK). .. The membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies at room temperature for 1 hour, and finally, the ECL chemiluminescence detection system (Servicebio, Wuhan, China) was scanned and analyzed via an image analysis system (Image Lab (TM) Touch Software, version 2.4.0.03, Bio-Rad, CA, US).

Article Title: Unraveling the crucial role of SDF-1 in osteoarthritis progression: IL6/HIF-1α positive feedback and chondrocyte ferroptosis.
Article Snippet: Background: Osteoarthritis (OA) is a common joint disease with an incompletely understood pathogenesis.. SDF-1, a key factor in cartilage matrix degradation, is involved in OA cartilage degeneration, yet its mechanism, especially regarding ferroptosis, remains unclear.. This study focuses on elucidating the role of SDF-1-induced chondrocyte ferroptosis and the IL6/HIF-1α signalling axis in OA.

Article Title: Curcumin ameliorates heatstroke-induced lung injury by activating the PI3K/AKT pathway.
Article Snippet: Heatstroke (HS) poses a significant threat to public health.. Curcumin, a polyphenolic compound, has been reported to possess anti-inflammatory and antioxidant properties.. This study aimed to investigate the potential therapeutic effects of curcumin on HS-induced lung injury and to elucidate its underlying molecular mechanisms.

Article Title: Acupotomy Ameliorates KOA Related Chondrocyte Premature Senescence Through YAP/FOXD1 Pathway
Article Snippet: A 12% SDS-PAGE (Nanjing Aoqing Bioengineering Institute, Nanjing, China) was used to load 3 μL of marker and 10 μL of protein samples at 160 V for 30 min. Proteins were then transferred to a 0.22 μm polyvinylidene difluoride (PVDF) membrane (Millipore, Boston, Mass, USA) at 400 mA for 25 minutes. .. The PVDF membrane was placed in a rapid blocking solution (Solarbio, Wuhan, China) at room temperature for 20 minutes, followed by overnight incubation at 4°C with primary antibodies against anti-P-YAP (1:1000, Ser127, Affbiotech, Cincinnati, OH, USA), YAP (1:1000, Bioss, Beijing, China), FOXD1 (1:2000, Bioss, Beijing, China), Rb1 (1:2000, Bioss, Beijing, China), p53 (1:1000, Proteintech, Chicago, United States) and GAPDH (1:10,000, Abcam, Cambridge, UK). .. The membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies at room temperature for 1 hour, and finally, the ECL chemiluminescence detection system (Servicebio, Wuhan, China) was scanned and analyzed via an image analysis system (Image Lab (TM) Touch Software, version 2.4.0.03, Bio-Rad, CA, US).

Article Title: Electroacupuncture Exerts Chondroprotective Effect in Knee Osteoarthritis of Rabbits Through the Mitophagy Pathway
Article Snippet: Electrophoresis was carried out with 12% SDS-polyacrylamide gel (SDSPAGE) at 80V for 2h, and then transferred to 0.22μm PVDF membranes (Millipore, Boston, Mass, USA) for 30 min at 100V. .. Subsequently, the PVDF membrane was placed in rapid blocking solution (Solarbio, Wuhan, China) for 20 min at room temperature. ..

Blocking Assay:

Article Title: lncRNA CHAF1B-2 contributes to the tumorigenesis of gastric cancer by activating the Wnt/β-catenin pathway.
Article Snippet: .. At room temperature, the membranes were blocked with protein-free rapid blocking solution (from Beijing Solarbio Science & Technology Co., Ltd.) for 15 min and then incubated overnight at 4 °C with primary antibodies targeting the following specific proteins: β-actin (1:1,000; Affinity, USA), β-catenin (1:1,000; CST, USA), GSK-3α/β (1:1,000; CST, USA), cyclin D1 (1:1,000; CST, USA), and c-myc (1:1,000; CST, USA). .. After a 2-hour incubation with secondary antibodies, the protein bands were subsequently visualized using a chemiluminescence (ECL) kit (from Beyotime Biotechnology, Shanghai).

Article Title: Acupotomy Ameliorates KOA Related Chondrocyte Premature Senescence Through YAP/FOXD1 Pathway
Article Snippet: A 12% SDS-PAGE (Nanjing Aoqing Bioengineering Institute, Nanjing, China) was used to load 3 μL of marker and 10 μL of protein samples at 160 V for 30 min. Proteins were then transferred to a 0.22 μm polyvinylidene difluoride (PVDF) membrane (Millipore, Boston, Mass, USA) at 400 mA for 25 minutes. .. The PVDF membrane was placed in a rapid blocking solution (Solarbio, Wuhan, China) at room temperature for 20 minutes, followed by overnight incubation at 4°C with primary antibodies against anti-P-YAP (1:1000, Ser127, Affbiotech, Cincinnati, OH, USA), YAP (1:1000, Bioss, Beijing, China), FOXD1 (1:2000, Bioss, Beijing, China), Rb1 (1:2000, Bioss, Beijing, China), p53 (1:1000, Proteintech, Chicago, United States) and GAPDH (1:10,000, Abcam, Cambridge, UK). .. The membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies at room temperature for 1 hour, and finally, the ECL chemiluminescence detection system (Servicebio, Wuhan, China) was scanned and analyzed via an image analysis system (Image Lab (TM) Touch Software, version 2.4.0.03, Bio-Rad, CA, US).

Incubation:

Article Title: Acupotomy Ameliorates KOA Related Chondrocyte Premature Senescence Through YAP/FOXD1 Pathway
Article Snippet: A 12% SDS-PAGE (Nanjing Aoqing Bioengineering Institute, Nanjing, China) was used to load 3 μL of marker and 10 μL of protein samples at 160 V for 30 min. Proteins were then transferred to a 0.22 μm polyvinylidene difluoride (PVDF) membrane (Millipore, Boston, Mass, USA) at 400 mA for 25 minutes. .. The PVDF membrane was placed in a rapid blocking solution (Solarbio, Wuhan, China) at room temperature for 20 minutes, followed by overnight incubation at 4°C with primary antibodies against anti-P-YAP (1:1000, Ser127, Affbiotech, Cincinnati, OH, USA), YAP (1:1000, Bioss, Beijing, China), FOXD1 (1:2000, Bioss, Beijing, China), Rb1 (1:2000, Bioss, Beijing, China), p53 (1:1000, Proteintech, Chicago, United States) and GAPDH (1:10,000, Abcam, Cambridge, UK). .. The membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies at room temperature for 1 hour, and finally, the ECL chemiluminescence detection system (Servicebio, Wuhan, China) was scanned and analyzed via an image analysis system (Image Lab (TM) Touch Software, version 2.4.0.03, Bio-Rad, CA, US).

other:

Article Title: Untargeted metabolomics and mendelian randomization analysis identify alpha-linolenic acid and linoleic acid as novel biomarkers of perioperative neurocognitive dysfunction.
Article Snippet: Untargeted metabolomics and mendelian randomization analysis identify alpha-linolenic acid and linoleic acid as novel biomarkers of perioperative neurocognitive dysfunction Xinxin Yang a, b, c, , Li Huang a, b, c, , Yanyan Zhang a, b, , Kaixin Wang a, b, , Shiya Liu a, b, , Xiaojin Li a, b, , Yuanyuan Ding a, b, , Daling Deng a, b, , Tianhao Zhang a, b, , Wenjing Zhao a, b, , Lulin Ma a, b, , Yafeng Wang a, b, , Shaofang Shu a, b, , Xiangdong Chen a, b, c, *

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