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profilerpro separa tion buffer  (Revvity)


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Bioz Manufacturer Symbol Revvity manufactures this product  
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    Structured Review

    Revvity profilerpro separa tion buffer
    Profilerpro Separa Tion Buffer, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/profilerpro+separation+buffer/LabChip+ProfilerPro+Separation+Buffer+(400+ml)+including+Coating+Reagent+8%2C+for+EZ+Reader/pm28723271-135-8-14
    Average 90 stars, based on 4 article reviews
    profilerpro separa tion buffer - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: A Stapled Peptide Mimic of the Pseudosubstrate Inhibitor PKI Inhibits Protein Kinase A
    Article Snippet: After 45 min (PKA-Cα or 20 min (PKA-Cβ) of incubation in the dark at RT, samples were drawn into a four-sipper mode ProfilerPro Chip (Perkin Elmer) using a LabChip DeskTop Profiler (Caliper Life Sciences).

    Article Title: A Stapled Peptide Mimic of the Pseudosubstrate Inhibitor PKI Inhibits Protein Kinase A
    Article Snippet: After 45 min (PKA-Cα or 20 min (PKA-Cβ) of incubation in the dark at RT, samples were drawn into a four-sipper mode ProfilerPro Chip (Perkin Elmer) using a LabChip DeskTop Profiler (Caliper Life Sciences).

    Article Title: Development of a high-throughput screening assay to identify inhibitors of the lipid kinase PIP5K1C
    Article Snippet: ProfilerPro separation buffer (760367) and coating-reagent 8 (CR-8; 760278) were purchased from PerkinElmer.

    Article Title: Development of Three Orthogonal Assays Suitable for the Identification and Qualification of PIKfyve Inhibitors.
    Article Snippet: FYVE-type zinc finger-containing phosphoinositide kinase (PIKfyve) catalyzes the formation of phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) from phosphatidylinositol 3-phosphate (PI(3)P).. PIKfyve has been implicated in multiple cellular processes, and its role in the regulation of toll-like receptor (TLR) pathways and the production of proinflammatory cytokines has sparked interest in developing small-molecule PIKfyve inhibitors as potential therapeutics to treat autoimmune and inflammatory diseases.. We developed three orthogonal assays to identify and qualify small-molecule inhibitors of PIKfyve: (1) a purified component microfluidic enzyme assay that measures the conversion of fluorescently labeled PI(3)P to PI(3,5)P2 by purified recombinant full-length human 6His-PIKfyve (rPIKfyve); (2) an intracellular protein stabilization assay using the kinase domain of PIKfyve expressed in HEK293 cells; and (3) a cell-based functional assay that measures the production of interleukin (IL)-12p70 in human peripheral blood mononuclear cells stimulated with TLR agonists lipopolysaccharide and R848.

    Article Title: Aurora Isoform Selectivity: Design and Synthesis of Imidazo[4,5-b]pyridine Derivatives as Highly Selective Inhibitors of Aurora-A Kinase in Cells
    Article Snippet: The assay was performed on an EZ Reader II (PerkinElmer Life Sciences, Waltham, MA) using separation buffer (no. 760367, PerkinElmer Life Sciences) containing CR-8 (500 nM, no. 760278, PerkinElmer Life Sciences).



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