pro co2 controller (BioSpherix)

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BioSpherix pro co2 controller

Figure 1. Hypercapnia limits AT2 cell proliferation in 3D culture organoids. (A) Schematic of experiments designed to coculture AT2 cells isolated from SftpCreERT2 R26REYFP mice (SftpcEYFP AT2) and WT mesenchymal cells. Alveolar organoids were switched to normocapnia (5% <t>CO2;</t> NC) or hypercapnia (20% CO2; HC) media on day 7 and cultured until day 21. (B) Representative images of organoid cultures in normocapnia or hypercapnia. Scale bars: 500 μm. (C) Graph depicts the inhibitory effect of hypercapnia on organoid size. Median with interquartile range. n = 8. (D) Graph depicts the effect of hypercapnia exposure for 21 days on colony forming efficiency (CFE). n = 8. (E) Immunofluorescence analysis of SFTPC (AT2 marker) and Podoplanin (AT1 marker) revealed a reduction in AT2 cell proliferation in organoids exposed to hypercapnia for 14 days relative to normocapnia. Nuclear DNA is stained with DAPI. Scale bars: 50 μm. (C) ANOVA plus Sidak’s multiple comparisons test. (D) Student’s t test. *P < 0.05; ***P < 0.001, ****P < 0.0001.

Pro Co2 Controller, supplied by BioSpherix, used in various techniques. Bioz Stars score: 96/100, based on 430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pro-co2+controller/C-Chamber/pm36626234-166-9-12
Average 96 stars, based on 430 article reviews

pro co2 controller - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells."

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

Journal: JCI insight

doi: 10.1172/jci.insight.159331

Figure Legend Snippet: Figure 1. Hypercapnia limits AT2 cell proliferation in 3D culture organoids. (A) Schematic of experiments designed to coculture AT2 cells isolated from SftpCreERT2 R26REYFP mice (SftpcEYFP AT2) and WT mesenchymal cells. Alveolar organoids were switched to normocapnia (5% CO2; NC) or hypercapnia (20% CO2; HC) media on day 7 and cultured until day 21. (B) Representative images of organoid cultures in normocapnia or hypercapnia. Scale bars: 500 μm. (C) Graph depicts the inhibitory effect of hypercapnia on organoid size. Median with interquartile range. n = 8. (D) Graph depicts the effect of hypercapnia exposure for 21 days on colony forming efficiency (CFE). n = 8. (E) Immunofluorescence analysis of SFTPC (AT2 marker) and Podoplanin (AT1 marker) revealed a reduction in AT2 cell proliferation in organoids exposed to hypercapnia for 14 days relative to normocapnia. Nuclear DNA is stained with DAPI. Scale bars: 50 μm. (C) ANOVA plus Sidak’s multiple comparisons test. (D) Student’s t test. *P < 0.05; ***P < 0.001, ****P < 0.0001.

Techniques Used: Isolation, Cell Culture, Immunofluorescence, Marker, Staining

Figure 2. Transcriptomic analysis of isolated AT2 cells reveals inhibition of βcat signaling during hypercapnia. (A) Hypercapnia decreases the number of cells expressing Ki67 in the alveolar region of the adult mouse lung exposed to room air (RA) or 10% CO2 (HC) for 21 days, as revealed by immunoflu- orescence. White arrows indicate SPTPC+Ki67+ AT2 cells. Scale bars: 20 μm. (B) Graph depicting the inhibitory effect of hypercapnia exposure for 21 days on proliferation. RA, n = 4; HC, n = 3 mice. Student’s t test. **P < 0.01. (C–F) Bulk RNA-Seq was performed on flow cytometry sorted AT2 cells from mice breathing RA (n = 6) or exposed to HC. Heatmap shows clustering of differentially expressed genes (FDR q < 0.05) in AT2 cells after 7 (n = 5) or 21 (n = 5) days of hypercapnia exposure. (D and E) Volcano plots. (F) GO biological processes. (G–J) Expression of selected DEG (FDR q < 0.05) regulated by hypercap- nia involved in the Wnt/βcat pathway.

Figure Legend Snippet: Figure 2. Transcriptomic analysis of isolated AT2 cells reveals inhibition of βcat signaling during hypercapnia. (A) Hypercapnia decreases the number of cells expressing Ki67 in the alveolar region of the adult mouse lung exposed to room air (RA) or 10% CO2 (HC) for 21 days, as revealed by immunoflu- orescence. White arrows indicate SPTPC+Ki67+ AT2 cells. Scale bars: 20 μm. (B) Graph depicting the inhibitory effect of hypercapnia exposure for 21 days on proliferation. RA, n = 4; HC, n = 3 mice. Student’s t test. **P < 0.01. (C–F) Bulk RNA-Seq was performed on flow cytometry sorted AT2 cells from mice breathing RA (n = 6) or exposed to HC. Heatmap shows clustering of differentially expressed genes (FDR q < 0.05) in AT2 cells after 7 (n = 5) or 21 (n = 5) days of hypercapnia exposure. (D and E) Volcano plots. (F) GO biological processes. (G–J) Expression of selected DEG (FDR q < 0.05) regulated by hypercap- nia involved in the Wnt/βcat pathway.

Techniques Used: Isolation, Inhibition, Expressing, RNA Sequencing, Flow Cytometry

Figure 3. Hypercapnia decreases Wnt/βcat signaling in AT2 cells. AT2 cells were isolated from mice exposed to room air (RA) or 10% CO2 (HC) for 21 days. (A) mRNA was isolated, and qPCR was performed. n = 8 mice. (B and C) In situ RNA hybridization showing decreased number of Axin2+ AT2 cells in mice exposed to HC. Yellow arrows indicate Sftpc+Axin2+ AT2 cells. Scale bars: 10 μm. n = 4 mice. (D) Number of lineage-labeled AT2 cells from Axin2CreERT2–TdTom mice determined by flow cytometry. n = 5 mice. Graph shows data from 1 of 3 independent experiments. Student’s t test. *P < 0.05; **P < 0.01.

Figure Legend Snippet: Figure 3. Hypercapnia decreases Wnt/βcat signaling in AT2 cells. AT2 cells were isolated from mice exposed to room air (RA) or 10% CO2 (HC) for 21 days. (A) mRNA was isolated, and qPCR was performed. n = 8 mice. (B and C) In situ RNA hybridization showing decreased number of Axin2+ AT2 cells in mice exposed to HC. Yellow arrows indicate Sftpc+Axin2+ AT2 cells. Scale bars: 10 μm. n = 4 mice. (D) Number of lineage-labeled AT2 cells from Axin2CreERT2–TdTom mice determined by flow cytometry. n = 5 mice. Graph shows data from 1 of 3 independent experiments. Student’s t test. *P < 0.05; **P < 0.01.

Techniques Used: Isolation, In Situ, Hybridization, Labeling, Flow Cytometry

Figure 4. Hypercapnia increases Wnt5a expression in PDGFRα+ fibroblasts. Lung PDGFRα+ fibroblasts were isolated via flow cytometry cell sorting from mice breathing room air (RA) or exposed to 10% CO2 (HC) for 10 days. (A) Expression of Wnt genes in PDGFRα+ fibroblasts as analyzed by population RNA-Seq. n = 3, with cells isolated from 3 mice in each replicate. #FDR q < 0.05). (B–D) mRNA was isolated, and qPCR was performed. (B) Wnt5a (n = 4). (C) Wnt2 (n = 3). (D) MLg2908 mouse lung fibroblast cells were preincubated in the presence or absence of UO126 (10 μM) or PD98059 (10 μM) for 90 minutes and exposed to media equilibrated to NC (5% CO2) or HC (20% CO2) for 24 hours. n = 3. (B and C) Student’s t test. (D) ANOVA plus Sidak’s multiple comparisons test. *P < 0.05; ** P < 0.01.

Figure Legend Snippet: Figure 4. Hypercapnia increases Wnt5a expression in PDGFRα+ fibroblasts. Lung PDGFRα+ fibroblasts were isolated via flow cytometry cell sorting from mice breathing room air (RA) or exposed to 10% CO2 (HC) for 10 days. (A) Expression of Wnt genes in PDGFRα+ fibroblasts as analyzed by population RNA-Seq. n = 3, with cells isolated from 3 mice in each replicate. #FDR q < 0.05). (B–D) mRNA was isolated, and qPCR was performed. (B) Wnt5a (n = 4). (C) Wnt2 (n = 3). (D) MLg2908 mouse lung fibroblast cells were preincubated in the presence or absence of UO126 (10 μM) or PD98059 (10 μM) for 90 minutes and exposed to media equilibrated to NC (5% CO2) or HC (20% CO2) for 24 hours. n = 3. (B and C) Student’s t test. (D) ANOVA plus Sidak’s multiple comparisons test. *P < 0.05; ** P < 0.01.

Techniques Used: Expressing, Isolation, Flow Cytometry, FACS, RNA Sequencing

Incubation:

Article Title: Nanobubble-Mediated Oxygen Delivery Mitigates Hypoxia-Induced ROS and HIF-1α Expression in UC-MSCs
Article Snippet: .. The cells were incubated in a hypoxic chamber (C-chamber, BioSpherix, NY, USA), while the control group was maintained under standard normoxic conditions (21% O 2 and 5% CO 2 ). .. To assess metabolic plasticity, UC-MSCs were treated for 24 h with metabolic inhibitors: 5 mM 2-Deoxy-D-glucose (2-DG; Sigma-Aldrich, Merck, Germany) to inhibit glycolysis or 1 μg/mL oligomycin (Calbiochem, Merck, Germany) to block mitochondrial oxidative phosphorylation.

Article Title: Nanobubble-Mediated Oxygen Delivery Mitigates Hypoxia-Induced ROS and HIF-1α Expression in UC-MSCs.
Article Snippet: .. The cells were incubated in a hypoxic chamber (C-chamber, BioSpherix, NY, USA), while the control group was maintained under standard normoxic conditions (21% O2 and 5% CO2). .. To assess metabolic plasticity, UC-MSCs were treated for 24 h with metabolic inhibitors: 5 mM 2-Deoxy-D-glucose (2-DG; Sigma-Aldrich, Merck, Germany) to inhibit glycolysis or 1 μg/mL oligomycin (Calbiochem, Merck, Germany) to block mitochondrial oxidative phosphorylation.

Control:

Cell Culture:

Article Title: TGM2-mediated serotonylation in tumor endothelial cells promotes angiogenesis and tumor growth in colorectal cancer.
Article Snippet: Angiogenesis fuels colorectal cancer (CRC) growth and metastasis by supplying oxygen and nutrients to expanding tumors.. Here, we define a TGM2-mediated serotonylation axis in tumor endothelial cells as a driver of angiogenesis and a key mediator of epigenetic reprogramming within the tumor microenvironment.. Across CRC single-cell datasets, TGM2 is broadly expressed in endothelial cells, and endothelial TGM2 correlates with the H3Q5Ser mark.

In Vitro:

Article Title: Hypercapnia-Regulated Molecular Pathways: Mechanistic Insights Into CO₂-Driven Cellular Signaling and Therapeutic Implications.
Article Snippet: Long dismissed as a passive marker of ventilatory failure, elevated carbon dioxide (PaCO2 > 45 mmHg) is now recognized as a potent signaling molecule that orchestrates complex cellular responses.. This review synthesizes recent advances revealing how hypercapnia modulates fundamental processes, immune regulation, tissue repair, and metabolism, through direct molecular mechanisms.. We detail how CO2 triggers noncanonical NF‐κB signaling, alters Wnt ligand secretion to impair alveolar regeneration, and exacerbates TLR4‐primed NLRP3 inflammasome activation; this pro‐inflammatory effect is most prominent in specific cell types like microglia and under conditions of sustained high CO2 levels.

Article Title: Systemic hypoxia suppresses solid tumor growth
Article Snippet: .. In vitro hypoxia experiments were carried out in C-Chambers (BioSpherix) controlled by a ProOx Model C21 (BioSpherix). ..

other:

Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxia in human brain organoids
Article Snippet: COs were exposed to low oxygen conditions on day 120 employing a hypoxic chamber (C-Chamber Three Shelf, Biospherix) and an oxygen controller (Pro Ox C21 Oxygen CO2 Single Chamber Controller).

Review