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luciferase vectors prl-null  (Promega)

 
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    Promega luciferase vectors prl-null
    a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the <t>pGL3</t> basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).
    Luciferase Vectors Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prl-+null+vector/pmc03594296-172-1-26?v=Promega
    Average 90 stars, based on 1 article reviews
    luciferase vectors prl-null - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells"

    Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0058500

    a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).
    Figure Legend Snippet: a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).

    Techniques Used: Construct, Plasmid Preparation, Luciferase

    a) Localization of the different Cx43 3′UTR constructs used in this work. All constructs contain the SV40 promoter (SV40-P) and the Luciferase coding region in addition to the pGL3 polyadenylation signal after the 3′UTR segments (not shown in graphics). The position of a putative polyadenylation signal is indicated. The S1516 region is shown in light grey. b, c) Luciferase assay of the first set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. d, e) Luciferase assay of the second set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05, reported to the pGL3-pr construct).
    Figure Legend Snippet: a) Localization of the different Cx43 3′UTR constructs used in this work. All constructs contain the SV40 promoter (SV40-P) and the Luciferase coding region in addition to the pGL3 polyadenylation signal after the 3′UTR segments (not shown in graphics). The position of a putative polyadenylation signal is indicated. The S1516 region is shown in light grey. b, c) Luciferase assay of the first set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. d, e) Luciferase assay of the second set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05, reported to the pGL3-pr construct).

    Techniques Used: Construct, Luciferase

    Luciferase assay in MCF7 cells transfected with different constructs including the pGL3 control, the pGL3-Pr, in addition to the full length 3′untranslated region (pGL3-Pr-3′UTR) or the S1516 regulatory element (pGL3-Pr-S1516). The experiments were performed at least three times in quadruplicates (* p <0.05).
    Figure Legend Snippet: Luciferase assay in MCF7 cells transfected with different constructs including the pGL3 control, the pGL3-Pr, in addition to the full length 3′untranslated region (pGL3-Pr-3′UTR) or the S1516 regulatory element (pGL3-Pr-S1516). The experiments were performed at least three times in quadruplicates (* p <0.05).

    Techniques Used: Luciferase, Transfection, Construct



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    Image Search Results


    a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).

    Journal: PLoS ONE

    Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells

    doi: 10.1371/journal.pone.0058500

    Figure Lengend Snippet: a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).

    Article Snippet: The luciferase vectors pGL3 (basic promoterless vector containing firefly luciferase), pGL3-pr (bearing an SV40 promoter) and pRL-Null (promoterless vector containing Renilla reniformis luciferase) were purchased from Promega (Madison, WI).

    Techniques: Construct, Plasmid Preparation, Luciferase

    a) Localization of the different Cx43 3′UTR constructs used in this work. All constructs contain the SV40 promoter (SV40-P) and the Luciferase coding region in addition to the pGL3 polyadenylation signal after the 3′UTR segments (not shown in graphics). The position of a putative polyadenylation signal is indicated. The S1516 region is shown in light grey. b, c) Luciferase assay of the first set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. d, e) Luciferase assay of the second set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05, reported to the pGL3-pr construct).

    Journal: PLoS ONE

    Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells

    doi: 10.1371/journal.pone.0058500

    Figure Lengend Snippet: a) Localization of the different Cx43 3′UTR constructs used in this work. All constructs contain the SV40 promoter (SV40-P) and the Luciferase coding region in addition to the pGL3 polyadenylation signal after the 3′UTR segments (not shown in graphics). The position of a putative polyadenylation signal is indicated. The S1516 region is shown in light grey. b, c) Luciferase assay of the first set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. d, e) Luciferase assay of the second set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05, reported to the pGL3-pr construct).

    Article Snippet: The luciferase vectors pGL3 (basic promoterless vector containing firefly luciferase), pGL3-pr (bearing an SV40 promoter) and pRL-Null (promoterless vector containing Renilla reniformis luciferase) were purchased from Promega (Madison, WI).

    Techniques: Construct, Luciferase

    Luciferase assay in MCF7 cells transfected with different constructs including the pGL3 control, the pGL3-Pr, in addition to the full length 3′untranslated region (pGL3-Pr-3′UTR) or the S1516 regulatory element (pGL3-Pr-S1516). The experiments were performed at least three times in quadruplicates (* p <0.05).

    Journal: PLoS ONE

    Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells

    doi: 10.1371/journal.pone.0058500

    Figure Lengend Snippet: Luciferase assay in MCF7 cells transfected with different constructs including the pGL3 control, the pGL3-Pr, in addition to the full length 3′untranslated region (pGL3-Pr-3′UTR) or the S1516 regulatory element (pGL3-Pr-S1516). The experiments were performed at least three times in quadruplicates (* p <0.05).

    Article Snippet: The luciferase vectors pGL3 (basic promoterless vector containing firefly luciferase), pGL3-pr (bearing an SV40 promoter) and pRL-Null (promoterless vector containing Renilla reniformis luciferase) were purchased from Promega (Madison, WI).

    Techniques: Luciferase, Transfection, Construct