kit components and software for data analysis of the multiplexed immunoassay (Myriad RBM)
90
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Myriad RBM
kit components and software for data analysis of the multiplexed immunoassay
Kit Components And Software For Data Analysis Of The Multiplexed Immunoassay, supplied by Myriad RBM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/principle+component+analysis+software/kit+components+and+software+for+data+analysis+of+the+multiplexed+immunoassay/pmc06858783-101-11-16
Average 90 stars, based on 1 article reviews
Kit Components And Software For Data Analysis Of The Multiplexed Immunoassay, supplied by Myriad RBM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/principle+component+analysis+software/kit+components+and+software+for+data+analysis+of+the+multiplexed+immunoassay/pmc06858783-101-11-16
Average 90 stars, based on 1 article reviews
kit components and software for data analysis of the multiplexed immunoassay - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer"
Article Title: A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer
Journal: Journal for Immunotherapy of Cancer
doi: 10.1186/s40425-019-0796-5
Figure Legend Snippet: Pam 3 Cys-GDPKHPKSF (XS15) is a TLR1/2 ligand activating immune cells and stimulating DCs and cytokine release . ( a ) Structure of Pam 3 Cys-GDPKHPKSF: Skeletal structural formula of the molecular structure of the lipopeptide Pam 3 Cys-GDPKHPKSF termed XS15. ( b ) Dual-luciferase assay on HEK293T cells transfected with TLR2: HEK293T cells were transiently transfected with a human TLR2 plasmid and a NF-κB luciferase reporter plasmid or left untreated (− ctrl.). Culture medium was replaced after 30 h and stimuli added at the stated concentrations. The cells were incubated for 18 h and lysates were prepared and analysed by dual-luciferase assay. Pam 3 CysSK 4 (P3CSK4) and two different lots of XS15 (XS15#1/ XS15#2) were used. ( c ) HEK-Dual hTLR2 cells, stably expressing a NF-κB/AP-1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter, were incubated for 1 h with TLR1, TLR2 and TLR6 blocking antibodies, isotype control or negative controls (no Abs) (4 μg/ml). Then, cells were stimulated for 24 h with the established TLR2/6 agonist FSL-1 (1 ng/ml), XS15 (10 ng/ml) or left unstimulated (− ctrl.). Supernatants were collected and SEAP levels determined using QUANTI-Blue detection assay. Error bars represent SD. The graph shows the mean + SEM of n = 2 experiments, significance was assessed by two-way ANOVA. ( d ) Immune cell activation by XS15: Fresh PBMCs were cultured for 40 h in the presence of Phytohemagglutinin-L (PHA) + Pokeweed (PWM) (P + P), Pam 3 CysSK 4 (P3CSK4), XS15 or left untreated (− ctrl.). Activated NK ( left panel ) and B cells ( right panel ) were assessed with the marker CD69 following the gating strategy: time gate, single cells (FSC-H/ FSC-A), living cells (Zombie-Aqua/ FSC-A), lymphocytes (FSC-A/ SSC-A); B-cells were defined as CD14 neg CD3 neg CD19 + cells and NK cells as CD14 neg CD3 neg CD19 neg CD56 + cells. Healthy donors ( n = 6), means are shown, significance was assessed by one-way ANOVA. ( e ) Dendritic cell (DC) stimulation by XS15: DCs were differentiated from blood monocytes and then matured as described in the material and methods section. Gating strategy was: time gate, single cells (FSC-H/ FSC-A), living cells (Zombie Aqua/ FSC-A). Upper panel : scatter plots for healthy donors ( n = 6), means are shown significance was assessed by one-way ANOVA. Lower panel : modal histograms and median fluorescences for one representative donor. Medium control without maturation cocktail = − ctrl. Standard maturation cocktail = Mat. ( f ) Induction of cytokine release by XS15: Anticoagulated whole blood was incubated with XS15 (10 μg/ml) as well as LPS (100 ng/ml) and PHA (2 μg/ml)/ PWM (1 μg/ml) as positive (+ ctrl.) and medium only as negative controls (− ctrl.) and supernatants harvested after 20 h. Multiplexed bead-based sandwich immunoassays were performed using a LUMINEX device with a 42-analyte panel. Exemplary findings obtained in three healthy donors (HD) for IL-8 ( left ), MCP1 ( middle ) and MIP-1β ( right ) are shown with means. HD1 (blue square) designates the vaccinated volunteer characterized in more detail subsequently. Additional results are provided in Additional file : Table S1. In case of saturation, the upper limit of quantification (ULOQ) was assigned. p ≤ 0.05*; ** p ≤ 0.01; *** p ≤ 0.001
Techniques Used: Luciferase, Transfection, Plasmid Preparation, Incubation, Stable Transfection, Expressing, Blocking Assay, Control, Detection Assay, Activation Assay, Cell Culture, Marker, Luminex
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