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LifeGene Inc primescripttm rt reagent kit
Primescripttm Rt Reagent Kit, supplied by LifeGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+reagent+kit/primescripttm+rt+reagent+kit/pmc10315057-84-29-33
Average 90 stars, based on 1 article reviews
primescripttm rt reagent kit - by Bioz Stars, 2026-10
90/100 stars

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RNA Extraction:

Article Title: Tryptophol Acetate and Tyrosol Acetate, Small-Molecule Metabolites Identified in a Probiotic Mixture, Inhibit Hyperinflammation
Article Snippet: NF‐κB (after 30 min) was assayed using cell lysates (70 μg), separated on SDS-PAGE and analyzed using the following: antibody raised against the anti-NF‐κB (p-65) (1:1,000; abcam, ab32536) and β-actin (1:1,000; MP Biomedicals, Santa Ana, CA, USA). .. Total mRNA from RAW 264.7 macrophages was extracted using a RNA extraction kit (ISOLATE II RNA Mini Kit, Bioline). cDNA was synthesized from 1 μg of RNA using a PrimeScriptTM RT reagent kit (Lifegene, Bio-52073). .. Subsequent real-time PCR was performed with an iCycler iQ TM Real-Time PCR Detection System (Bio-Rad).

Synthesized:

Article Title: Tryptophol Acetate and Tyrosol Acetate, Small-Molecule Metabolites Identified in a Probiotic Mixture, Inhibit Hyperinflammation
Article Snippet: NF‐κB (after 30 min) was assayed using cell lysates (70 μg), separated on SDS-PAGE and analyzed using the following: antibody raised against the anti-NF‐κB (p-65) (1:1,000; abcam, ab32536) and β-actin (1:1,000; MP Biomedicals, Santa Ana, CA, USA). .. Total mRNA from RAW 264.7 macrophages was extracted using a RNA extraction kit (ISOLATE II RNA Mini Kit, Bioline). cDNA was synthesized from 1 μg of RNA using a PrimeScriptTM RT reagent kit (Lifegene, Bio-52073). .. Subsequent real-time PCR was performed with an iCycler iQ TM Real-Time PCR Detection System (Bio-Rad).



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