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Ribobio co prime script rt reagent kit
Prime Script Rt Reagent Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+reagent+kit/primescripttm+rt+reagent+kit/pm39504594-79-6-11
Average 90 stars, based on 1 article reviews
prime script rt reagent kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Synthesized:

Article Title: Promotion of Bronchopulmonary Dysplasia Progression Using Circular RNA circabcc4 via Facilitating PLA2G6 Expression by Sequestering miR-663a.
Article Snippet: For circRNA and mRNA, RNA was reversely transcribed into cDNA using a PrimeScriptTM RT Master Mix reagent kit (TaKaRa, Shiga, Japan). .. For miRNA, cDNA was synthesized by the PrimeScriptTM RT reagent kit (RiboBio, GuangZhou, China). qRT-PCR was performed in the LightCycler480II (Roche) using SYBR Green technology (Takara). ..

Article Title: Promotion of Bronchopulmonary Dysplasia Progression Using Circular RNA circabcc4 via Facilitating PLA2G6 Expression by Sequestering miR-663a
Article Snippet: For circRNA and mRNA, RNA was reversely transcribed into cDNA using a PrimeScript TM RT Master Mix reagent kit (TaKaRa, Shiga, Japan). .. For miRNA, cDNA was synthesized by the PrimeScript TM RT reagent kit (RiboBio, GuangZhou, China). qRT-PCR was performed in the LightCycler480II (Roche) using SYBR Green technology (Takara). ..

Article Title: CircPICALM promotes neonatal acute kidney injury triggered by hypoxia/reoxygenation via sponging microRNA-204-5p.
Article Snippet: Background: Circular RNAs (circRNAs) have been documented to regulate neonatal acute kidney injury (AKI).. Based on previous RNA-sequence findings, circPICALM exhibited significantly disparate expression between AKI newborns and Controls.. This study aimed to provide further insights into the regulatory mechanism of circPICALM in neonatal AKI.

Quantitative RT-PCR:

Article Title: Promotion of Bronchopulmonary Dysplasia Progression Using Circular RNA circabcc4 via Facilitating PLA2G6 Expression by Sequestering miR-663a.
Article Snippet: For circRNA and mRNA, RNA was reversely transcribed into cDNA using a PrimeScriptTM RT Master Mix reagent kit (TaKaRa, Shiga, Japan). .. For miRNA, cDNA was synthesized by the PrimeScriptTM RT reagent kit (RiboBio, GuangZhou, China). qRT-PCR was performed in the LightCycler480II (Roche) using SYBR Green technology (Takara). ..

Article Title: Promotion of Bronchopulmonary Dysplasia Progression Using Circular RNA circabcc4 via Facilitating PLA2G6 Expression by Sequestering miR-663a
Article Snippet: For circRNA and mRNA, RNA was reversely transcribed into cDNA using a PrimeScript TM RT Master Mix reagent kit (TaKaRa, Shiga, Japan). .. For miRNA, cDNA was synthesized by the PrimeScript TM RT reagent kit (RiboBio, GuangZhou, China). qRT-PCR was performed in the LightCycler480II (Roche) using SYBR Green technology (Takara). ..

SYBR Green Assay:

Article Title: Promotion of Bronchopulmonary Dysplasia Progression Using Circular RNA circabcc4 via Facilitating PLA2G6 Expression by Sequestering miR-663a.
Article Snippet: For circRNA and mRNA, RNA was reversely transcribed into cDNA using a PrimeScriptTM RT Master Mix reagent kit (TaKaRa, Shiga, Japan). .. For miRNA, cDNA was synthesized by the PrimeScriptTM RT reagent kit (RiboBio, GuangZhou, China). qRT-PCR was performed in the LightCycler480II (Roche) using SYBR Green technology (Takara). ..

Article Title: Promotion of Bronchopulmonary Dysplasia Progression Using Circular RNA circabcc4 via Facilitating PLA2G6 Expression by Sequestering miR-663a
Article Snippet: For circRNA and mRNA, RNA was reversely transcribed into cDNA using a PrimeScript TM RT Master Mix reagent kit (TaKaRa, Shiga, Japan). .. For miRNA, cDNA was synthesized by the PrimeScript TM RT reagent kit (RiboBio, GuangZhou, China). qRT-PCR was performed in the LightCycler480II (Roche) using SYBR Green technology (Takara). ..

RNA Extraction:

Article Title: The Role of miR-107 as a Potential Biomarker and Cellular Factor for Acute Aortic Dissection.
Article Snippet: Acute aortic dissection (AD) is one of the most severe and highly mortality vascular disease.. Its actual prevalence may be seriously underestimated.. We studied different expression genes to understand gene profile change between acute AD and nondiseased individuals, and then discover potential biomarkers and therapeutic targets of acute AD.

Real-time Polymerase Chain Reaction:

Article Title: The Role of miR-107 as a Potential Biomarker and Cellular Factor for Acute Aortic Dissection.
Article Snippet: Acute aortic dissection (AD) is one of the most severe and highly mortality vascular disease.. Its actual prevalence may be seriously underestimated.. We studied different expression genes to understand gene profile change between acute AD and nondiseased individuals, and then discover potential biomarkers and therapeutic targets of acute AD.

Reverse Transcription:

Article Title: The Role of miR-107 as a Potential Biomarker and Cellular Factor for Acute Aortic Dissection.
Article Snippet: Acute aortic dissection (AD) is one of the most severe and highly mortality vascular disease.. Its actual prevalence may be seriously underestimated.. We studied different expression genes to understand gene profile change between acute AD and nondiseased individuals, and then discover potential biomarkers and therapeutic targets of acute AD.

Article Title: Down-regulation of Exosomal miR-214-3p Targeting CCN2 Contributes to Endometriosis Fibrosis and the Role of Exosomes in the Horizontal Transfer of miR-214-3p.
Article Snippet: Endometriosis (EMs) is defined as the presence of tissue which somewhat resembles endometrial glands and stroma outside the uterus, and elicits fibrosis.. Fibrosis is the main factor resulting in pain and infertility, while the aetiology of endometrial fibrosis is unknown.. There is strong evidence from numerous experiments showing that connective tissue growth factor (CCN2) plays a central role in fibrogenesis.

Article Title: LncRNA H19 sponges miR-103-3p to promote the high phosphorus-induced osteoblast phenotypic transition of vascular smooth muscle cells by upregulating Runx2.
Article Snippet: Elucidating the mechanism of the osteogenic phenotypic transdifferentiation of vascular smooth muscle cells (VSMCs) is the key to determining the diagnosis and treatment of arterial medial calcification (AMC).. Long noncoding RNAs (lncRNAs) have been reported to participate in the regulation of vascular physiology and pathology.. Here, we investigated the effect and mechanism of the lncRNA H19 on the osteoblastic differentiation of VSMCs induced by high phosphorus.

Article Title: A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse model.
Article Snippet: .. Reverse transcription of miRNA and mRNA from 1 μg of total RNA was performed using a first-strand cDNA synthesis kit and a PrimeScript RT kit (RiboBio C10712-2), respectively, and the resulting products were subsequently used as templates for PCR amplification. ..

Isolation:

Article Title: Down-regulation of Exosomal miR-214-3p Targeting CCN2 Contributes to Endometriosis Fibrosis and the Role of Exosomes in the Horizontal Transfer of miR-214-3p.
Article Snippet: Endometriosis (EMs) is defined as the presence of tissue which somewhat resembles endometrial glands and stroma outside the uterus, and elicits fibrosis.. Fibrosis is the main factor resulting in pain and infertility, while the aetiology of endometrial fibrosis is unknown.. There is strong evidence from numerous experiments showing that connective tissue growth factor (CCN2) plays a central role in fibrogenesis.

Polymerase Chain Reaction:

Article Title: LncRNA H19 sponges miR-103-3p to promote the high phosphorus-induced osteoblast phenotypic transition of vascular smooth muscle cells by upregulating Runx2.
Article Snippet: Elucidating the mechanism of the osteogenic phenotypic transdifferentiation of vascular smooth muscle cells (VSMCs) is the key to determining the diagnosis and treatment of arterial medial calcification (AMC).. Long noncoding RNAs (lncRNAs) have been reported to participate in the regulation of vascular physiology and pathology.. Here, we investigated the effect and mechanism of the lncRNA H19 on the osteoblastic differentiation of VSMCs induced by high phosphorus.

Article Title: A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse model.
Article Snippet: .. Reverse transcription of miRNA and mRNA from 1 μg of total RNA was performed using a first-strand cDNA synthesis kit and a PrimeScript RT kit (RiboBio C10712-2), respectively, and the resulting products were subsequently used as templates for PCR amplification. ..

cDNA Synthesis:

Article Title: A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse model.
Article Snippet: .. Reverse transcription of miRNA and mRNA from 1 μg of total RNA was performed using a first-strand cDNA synthesis kit and a PrimeScript RT kit (RiboBio C10712-2), respectively, and the resulting products were subsequently used as templates for PCR amplification. ..

Amplification:

Article Title: A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse model.
Article Snippet: .. Reverse transcription of miRNA and mRNA from 1 μg of total RNA was performed using a first-strand cDNA synthesis kit and a PrimeScript RT kit (RiboBio C10712-2), respectively, and the resulting products were subsequently used as templates for PCR amplification. ..



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