primescripttm rt mastermix kit (Toyobo)
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primescripttm rt mastermix kit
Primescripttm Rt Mastermix Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+mastermix+kit/primescript+rt+master+mix/pmc09966794-147-14-18
Average 90 stars, based on 1 article reviews
Primescripttm Rt Mastermix Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+mastermix+kit/primescript+rt+master+mix/pmc09966794-147-14-18
Average 90 stars, based on 1 article reviews
primescripttm rt mastermix kit - by Bioz Stars,
2026-10
90/100 stars
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Reverse Transcription:Article Title: Diabetic Macrophage Exosomal miR-381-3p Inhibits Epithelial Cell Autophagy Via NR5A2. Article Snippet: Total RNA was extracted from cells using Nucleozol Reagent (Gene Company Limited, Hong Kong, China). .. The RNA was then reverse transcribed to generate first-strand cDNA using the Article Title: FoxO1 mediates odontoblast differentiation of hDPSCs via B cell-derived ANGPTL1 in dental caries: A laboratory investigation. Article Snippet: Int Endod J.. 2025;00:1–19.. | 1wileyonlinelibrary.com/journal/iej © 2025 British Endodontic Society. Article Title: Diabetic Macrophage Exosomal miR-381-3p Inhibits Epithelial Cell Autophagy Via NR5A2 Article Snippet: Total RNA was extracted from cells using Nucleozol Reagent (Gene Company Limited, Hong Kong, China). .. The RNA was then reverse transcribed to generate first-strand cDNA using the Real-time Polymerase Chain Reaction:Article Title: FoxO1 mediates odontoblast differentiation of hDPSCs via B cell-derived ANGPTL1 in dental caries: A laboratory investigation. Article Snippet: Int Endod J.. 2025;00:1–19.. | 1wileyonlinelibrary.com/journal/iej © 2025 British Endodontic Society. Quantitative RT-PCR:Article Title: FoxO1 mediates odontoblast differentiation of hDPSCs via B cell-derived ANGPTL1 in dental caries: A laboratory investigation. Article Snippet: Int Endod J.. 2025;00:1–19.. | 1wileyonlinelibrary.com/journal/iej © 2025 British Endodontic Society. Generated:Article Title: FoxO1 mediates odontoblast differentiation of hDPSCs via B cell-derived ANGPTL1 in dental caries: A laboratory investigation. Article Snippet: Int Endod J.. 2025;00:1–19.. | 1wileyonlinelibrary.com/journal/iej © 2025 British Endodontic Society. Article Title: Ferroptosis of macrophages facilitates bone loss in apical periodontitis via NRF2/FSP1/ROS pathway. Article Snippet: RAW 264.7 cells were prestimulated with 500 ng/mL LPS for 24 h. The supernatants of LPSstimulated RAW 264.7 macrophages were pre-treated with 10 μg/ml neutralizing antibodies against TNF-α (eBioscienceTM TN3-19.12, Thermo Fisher Scientific) for 30 min [33], then co-cultured with BMSCs as previously described. .. Total RNA was extracted from cells using an RNA extraction kit (Yishan Biotechnology, Shanghai, China) according to the product instructions, and cDNA was generated with Isolation:Article Title: Blocking CXCR1/2 attenuates experimental periodontitis by suppressing neutrophils recruitment. Article Snippet: Periodontitis (PD) is a common chronic oral inflammatory disease that cause alveolar bone loss.. Current strategies for bone regeneration achieve limited results in PD.. The aberrant host osteoimmunity to pathogenic bacteria is responsible for the destruction of alveolar bone in PD. Article Title: Restoring periodontal tissue homoeostasis prevents cognitive decline by reducing the number of Serpina3n high astrocytes in the hippocampus Article Snippet: To assess the suppressive 255 effects of NPsiNLRP3 on NLRP3 inflammasome activation, BMDMs were transfected 256 with NPsiNLRP3 or other formulations for 6 h. The NPs were loaded with 25 nM, 50 nM, 257 100 nM or 200 nM siNLRP3, while 100 nM free siNLPR3 and NPsiNC were used as 258 controls. .. To activate the NLRP3 inflammasome, 72 h after transfection, the cells were 259 primed with 500 ng/ml LPS for 3 h and were then stimulated with nigericin (10 μM) 260 for 30 min. After incubation, BMDMs and the culture supernatant were separated, and 261 BMDMs were collected by centrifugation at 400× g for 5 min. Total RNA was isolated 262 from BMDMs with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and cDNA was 263 synthesized using Transfection:Article Title: Restoring periodontal tissue homoeostasis prevents cognitive decline by reducing the number of Serpina3n high astrocytes in the hippocampus Article Snippet: To assess the suppressive 255 effects of NPsiNLRP3 on NLRP3 inflammasome activation, BMDMs were transfected 256 with NPsiNLRP3 or other formulations for 6 h. The NPs were loaded with 25 nM, 50 nM, 257 100 nM or 200 nM siNLRP3, while 100 nM free siNLPR3 and NPsiNC were used as 258 controls. .. To activate the NLRP3 inflammasome, 72 h after transfection, the cells were 259 primed with 500 ng/ml LPS for 3 h and were then stimulated with nigericin (10 μM) 260 for 30 min. After incubation, BMDMs and the culture supernatant were separated, and 261 BMDMs were collected by centrifugation at 400× g for 5 min. Total RNA was isolated 262 from BMDMs with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and cDNA was 263 synthesized using Incubation:Article Title: Restoring periodontal tissue homoeostasis prevents cognitive decline by reducing the number of Serpina3n high astrocytes in the hippocampus Article Snippet: To assess the suppressive 255 effects of NPsiNLRP3 on NLRP3 inflammasome activation, BMDMs were transfected 256 with NPsiNLRP3 or other formulations for 6 h. The NPs were loaded with 25 nM, 50 nM, 257 100 nM or 200 nM siNLRP3, while 100 nM free siNLPR3 and NPsiNC were used as 258 controls. .. To activate the NLRP3 inflammasome, 72 h after transfection, the cells were 259 primed with 500 ng/ml LPS for 3 h and were then stimulated with nigericin (10 μM) 260 for 30 min. After incubation, BMDMs and the culture supernatant were separated, and 261 BMDMs were collected by centrifugation at 400× g for 5 min. Total RNA was isolated 262 from BMDMs with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and cDNA was 263 synthesized using Centrifugation:Article Title: Restoring periodontal tissue homoeostasis prevents cognitive decline by reducing the number of Serpina3n high astrocytes in the hippocampus Article Snippet: To assess the suppressive 255 effects of NPsiNLRP3 on NLRP3 inflammasome activation, BMDMs were transfected 256 with NPsiNLRP3 or other formulations for 6 h. The NPs were loaded with 25 nM, 50 nM, 257 100 nM or 200 nM siNLRP3, while 100 nM free siNLPR3 and NPsiNC were used as 258 controls. .. To activate the NLRP3 inflammasome, 72 h after transfection, the cells were 259 primed with 500 ng/ml LPS for 3 h and were then stimulated with nigericin (10 μM) 260 for 30 min. After incubation, BMDMs and the culture supernatant were separated, and 261 BMDMs were collected by centrifugation at 400× g for 5 min. Total RNA was isolated 262 from BMDMs with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and cDNA was 263 synthesized using Synthesized:Article Title: Restoring periodontal tissue homoeostasis prevents cognitive decline by reducing the number of Serpina3n high astrocytes in the hippocampus Article Snippet: To assess the suppressive 255 effects of NPsiNLRP3 on NLRP3 inflammasome activation, BMDMs were transfected 256 with NPsiNLRP3 or other formulations for 6 h. The NPs were loaded with 25 nM, 50 nM, 257 100 nM or 200 nM siNLRP3, while 100 nM free siNLPR3 and NPsiNC were used as 258 controls. .. To activate the NLRP3 inflammasome, 72 h after transfection, the cells were 259 primed with 500 ng/ml LPS for 3 h and were then stimulated with nigericin (10 μM) 260 for 30 min. After incubation, BMDMs and the culture supernatant were separated, and 261 BMDMs were collected by centrifugation at 400× g for 5 min. Total RNA was isolated 262 from BMDMs with TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and cDNA was 263 synthesized using Article Title: Multiple exposures to low-dose ionizing radiation induced the initiation and progression of pro-atherosclerotic phenotypes in mice and vascular endothelial cell damage. Article Snippet: Total RNA extraction from the cells was carried out utilizing Trizol (Invitrogen). .. Subsequently, cDNA was synthesized from 1 μg of total RNA employing the Article Title: Multiple exposures to low-dose ionizing radiation induced the initiation and progression of pro-atherosclerotic phenotypes in mice and vascular endothelial cell damage Article Snippet: Total RNA extraction from the cells was carried out utilizing Trizol (Invitrogen). .. Subsequently, cDNA was synthesized from 1 μg of total RNA employing the RNA Extraction:Article Title: Ferroptosis of macrophages facilitates bone loss in apical periodontitis via NRF2/FSP1/ROS pathway. Article Snippet: RAW 264.7 cells were prestimulated with 500 ng/mL LPS for 24 h. The supernatants of LPSstimulated RAW 264.7 macrophages were pre-treated with 10 μg/ml neutralizing antibodies against TNF-α (eBioscienceTM TN3-19.12, Thermo Fisher Scientific) for 30 min [33], then co-cultured with BMSCs as previously described. .. Total RNA was extracted from cells using an RNA extraction kit (Yishan Biotechnology, Shanghai, China) according to the product instructions, and cDNA was generated with |