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primescripttm rt mastermix kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher primescripttm rt mastermix kit
    Primescripttm Rt Mastermix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+mastermix+kit/primescripttm+rt+mastermix+kit/pm34951563-45-8-12
    Average 90 stars, based on 1 article reviews
    primescripttm rt mastermix kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Depleting circ_0088364 restrained cell growth and motility of human hepatocellular carcinoma via circ_0088364-miR-1270-COL4A1 ceRNA pathway.
    Article Snippet: The RNA of tissues or cells was separated according to producer’s protocols of TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. Complementary DNA (cDNA) was synthesized and amplified using PrimeScriptTM RT MasterMix kit (Invitrogen) and SYBR Green qRT-PCR Master Mixed (Thermo Fisher Scientific, Waltham, MA, USA), respectively. .. Threshold cycle (Ct) values of circ_0088364, miR1270 and COL4A1 were determined on ABI 7500 thermal cycler (Applied Biosystems, Foster City, CA, USA), as well as the internal controls including β-actin and U6 small nuclear RNA (U6).

    Article Title: LINC00565 promotes the progression of colorectal cancer by upregulating EZH2
    Article Snippet: Total RNA was extracted from the tissues and cells using the TRIzol kit (Invitrogen; Thermo Fisher Scientific Inc.), respectively, followed by measurement of RNA concentration using an ultraviolet spectrophotometer (Hitachi Ltd.). .. The complementary Deoxyribose Nucleic Acid (cDNA) was synthesized according to the instructions of the PrimeScriptTM RT MasterMix kit (Invitrogen; Thermo Fisher Scientific Inc.). qPCR was subsequently conducted following the recommendations of SYBR Premix Ex TaqTM (Takara Bio, Inc.). ..

    Amplification:

    Article Title: Depleting circ_0088364 restrained cell growth and motility of human hepatocellular carcinoma via circ_0088364-miR-1270-COL4A1 ceRNA pathway.
    Article Snippet: The RNA of tissues or cells was separated according to producer’s protocols of TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. Complementary DNA (cDNA) was synthesized and amplified using PrimeScriptTM RT MasterMix kit (Invitrogen) and SYBR Green qRT-PCR Master Mixed (Thermo Fisher Scientific, Waltham, MA, USA), respectively. .. Threshold cycle (Ct) values of circ_0088364, miR1270 and COL4A1 were determined on ABI 7500 thermal cycler (Applied Biosystems, Foster City, CA, USA), as well as the internal controls including β-actin and U6 small nuclear RNA (U6).

    SYBR Green Assay:

    Article Title: Depleting circ_0088364 restrained cell growth and motility of human hepatocellular carcinoma via circ_0088364-miR-1270-COL4A1 ceRNA pathway.
    Article Snippet: The RNA of tissues or cells was separated according to producer’s protocols of TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. Complementary DNA (cDNA) was synthesized and amplified using PrimeScriptTM RT MasterMix kit (Invitrogen) and SYBR Green qRT-PCR Master Mixed (Thermo Fisher Scientific, Waltham, MA, USA), respectively. .. Threshold cycle (Ct) values of circ_0088364, miR1270 and COL4A1 were determined on ABI 7500 thermal cycler (Applied Biosystems, Foster City, CA, USA), as well as the internal controls including β-actin and U6 small nuclear RNA (U6).

    Quantitative RT-PCR:

    Article Title: Depleting circ_0088364 restrained cell growth and motility of human hepatocellular carcinoma via circ_0088364-miR-1270-COL4A1 ceRNA pathway.
    Article Snippet: The RNA of tissues or cells was separated according to producer’s protocols of TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. Complementary DNA (cDNA) was synthesized and amplified using PrimeScriptTM RT MasterMix kit (Invitrogen) and SYBR Green qRT-PCR Master Mixed (Thermo Fisher Scientific, Waltham, MA, USA), respectively. .. Threshold cycle (Ct) values of circ_0088364, miR1270 and COL4A1 were determined on ABI 7500 thermal cycler (Applied Biosystems, Foster City, CA, USA), as well as the internal controls including β-actin and U6 small nuclear RNA (U6).

    Real-time Polymerase Chain Reaction:

    Article Title: LINC00565 promotes the progression of colorectal cancer by upregulating EZH2
    Article Snippet: Total RNA was extracted from the tissues and cells using the TRIzol kit (Invitrogen; Thermo Fisher Scientific Inc.), respectively, followed by measurement of RNA concentration using an ultraviolet spectrophotometer (Hitachi Ltd.). .. The complementary Deoxyribose Nucleic Acid (cDNA) was synthesized according to the instructions of the PrimeScriptTM RT MasterMix kit (Invitrogen; Thermo Fisher Scientific Inc.). qPCR was subsequently conducted following the recommendations of SYBR Premix Ex TaqTM (Takara Bio, Inc.). ..



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