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EZBioscience primescript rt kit
Primescript Rt Kit, supplied by EZBioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt-qpcr+kit/color+reverse+transcription+kit/ppr0834979-61-0-3
Average 90 stars, based on 1 article reviews
primescript rt kit - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Identification of pyroptosis related genes and subtypes in atherosclerosis using multiomic and single cell analysis.
Article Snippet: Real-Time quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted using the Tissue RNA Purification Kit PLUS (EZB-RN001-plus, EZBioscience, China) or the EZ-press RNA Purification Kit (B0004D, EZBioscience) according to the manufacturer’s instructions.

Article Title: Surface-Engineered Umbilical Cord Mesenchymal Stem Cell-Derived sEVs for Targeted Therapy of Osteoarthritis.
Article Snippet: Small extracellular vesicles (sEVs) from human umbilical-cord-derived mesenchymal stem cells (UCMSCs) hold promise for cartilage regeneration in osteoarthritis (OA).. However, their therapeutic effectiveness is significantly limited by rapid clearance through blood and lymphatic vessels in the synovial tissue after intra-articular injection.. This study aimed to enhance the chondrocyte-targeting ability and improve the cartilage repair potential of UCMSC-derived sEVs by conjugating them with a chondrocyte-affinity peptide (CAP) using copper-free click chemistry.

Reverse Transcription:

Article Title: Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly.
Article Snippet: Total RNA was extracted via an EZ-press RNA Purification Kit (EZBioscience). .. Reverse transcription and qRT-PCR were performed via a Color Reverse Transcription Kit (EZBioscience) and 2× Color SYBR Green qPCR Master Mix (EZBioscience) according to the manufacturer’s instructions. ..

Article Title: ATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity.
Article Snippet: After 2 days of induction, total RNA was extracted from the cells using the EZ-press RNA Purification Kit (EZBiosciences) according to the manufacturer’s instructions. .. The total RNA was reverse-transcribed using the Color Reverse Transcription Kit (A0010CGQ, EZBioscience). cDNA amplification was performed using 2 £ SYBR Green qPCR Master Mix (A0012-R1, EZBioscience). ..

Article Title: Circ-0000197 derived from porcine milk small extracellular vesicles promotes intestinal barrier function by sponging miR-429.
Article Snippet: Briefly, total RNA was isolated by TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s protocol. .. The cDNAs were obtained by Color Reverse Transcription Kit (EZBioscience, CA, USA). .. Quantification of mRNA, miRNA and circRNA was performed by a SBRY Green PCR Kit (Takara, Shiga, Japan).

Article Title: ATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity
Article Snippet: After 2 days of induction, total RNA was extracted from the cells using the EZ-press RNA Purification Kit (EZBiosciences) according to the manufacturer’s instructions. .. The total RNA was reverse-transcribed using the Color Reverse Transcription Kit (A0010CGQ, EZBioscience). .. cDNA amplification was performed using 2 × SYBR Green qPCR Master Mix (A0012-R1, EZBioscience).

Article Title: Genome-wide analyses identify circAQR, a circular RNA suppressing proliferation but promoting migration and invasion in thyroid cancer.
Article Snippet: J circAQR expression accelerated themigration and invasion of BCPAP compared to the negative control group, as assessed by the Transwell assay. npj Precision Oncology | (2025) 9:171 9 Qiao Xin Zhou Biotechnology Co., Ltd. All cell lines were confirmed to be mycoplasma negative and were supplemented with 10% fetal bovine serum (Gibco, USA), 1% penicillin/streptomycin with RPMI 1640 medium at 37 °C,95% O2 and 5% CO2. .. We used EZ-press RNA Purification Kit (EZBioscience, USA) to extract total RNA from the cell lines according to the manufacturer’s instructions. cDNA was synthesized by using the Color Reverse Transcription Kit (EZBioscience, USA) according to the manufacturer’s instructions. .. Then, quantitative real-time PCR (qRT-PCR) was performed using SYBR Green qPCRMasterMixKit (EZBioscience,USA) according to themanufacturer’s instructions.

Article Title: Loss of TACC2 impairs chemokine CCL3 and CCL4 expression and reduces response to anti-PD-1 therapy in soft tissue sarcoma.
Article Snippet: The antibodies used included anti-TACC2 (11407-1-AP, Proteintech), anti-Ki-67 (ab16667, Abcam), anti-CD4 (ab183685, Abcam), anti-CD8 (ab209775, Abcam), and anti-CD11b (ab133357, Abcam), along with goat anti-rabbit (PV6001, ZSGB-BIO) and goat anti-mouse (PV-6002, ZSGBBIO) secondary antibodies. .. Total RNA was extracted from tissues and cells using the TRIzolTM reagent (Sigma-Aldrich), and cDNAs were synthesized with the A0010CG Color Reverse Transcription Kit (EZBioscience, China) according to the manufacturer’s protocol. .. Quantitative real-time PCR (qRT-PCR) was performed using SYBR Green Master Mix (EZBioscience) on a Bio-Rad CFX96 PCR instrument.

Quantitative RT-PCR:

Article Title: Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly.
Article Snippet: Total RNA was extracted via an EZ-press RNA Purification Kit (EZBioscience). .. Reverse transcription and qRT-PCR were performed via a Color Reverse Transcription Kit (EZBioscience) and 2× Color SYBR Green qPCR Master Mix (EZBioscience) according to the manufacturer’s instructions. ..

SYBR Green Assay:

Article Title: Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly.
Article Snippet: Total RNA was extracted via an EZ-press RNA Purification Kit (EZBioscience). .. Reverse transcription and qRT-PCR were performed via a Color Reverse Transcription Kit (EZBioscience) and 2× Color SYBR Green qPCR Master Mix (EZBioscience) according to the manufacturer’s instructions. ..

Article Title: ATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity.
Article Snippet: After 2 days of induction, total RNA was extracted from the cells using the EZ-press RNA Purification Kit (EZBiosciences) according to the manufacturer’s instructions. .. The total RNA was reverse-transcribed using the Color Reverse Transcription Kit (A0010CGQ, EZBioscience). cDNA amplification was performed using 2 £ SYBR Green qPCR Master Mix (A0012-R1, EZBioscience). ..

Real-time Polymerase Chain Reaction:

Article Title: Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly.
Article Snippet: Total RNA was extracted via an EZ-press RNA Purification Kit (EZBioscience). .. Reverse transcription and qRT-PCR were performed via a Color Reverse Transcription Kit (EZBioscience) and 2× Color SYBR Green qPCR Master Mix (EZBioscience) according to the manufacturer’s instructions. ..

Article Title: ATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity.
Article Snippet: After 2 days of induction, total RNA was extracted from the cells using the EZ-press RNA Purification Kit (EZBiosciences) according to the manufacturer’s instructions. .. The total RNA was reverse-transcribed using the Color Reverse Transcription Kit (A0010CGQ, EZBioscience). cDNA amplification was performed using 2 £ SYBR Green qPCR Master Mix (A0012-R1, EZBioscience). ..

Amplification:

Article Title: ATP1A1-Driven Intercellular Contact Between Dental Pulp Stem Cell and Endothelial Cell Enhances Vasculogenic Activity.
Article Snippet: After 2 days of induction, total RNA was extracted from the cells using the EZ-press RNA Purification Kit (EZBiosciences) according to the manufacturer’s instructions. .. The total RNA was reverse-transcribed using the Color Reverse Transcription Kit (A0010CGQ, EZBioscience). cDNA amplification was performed using 2 £ SYBR Green qPCR Master Mix (A0012-R1, EZBioscience). ..

Purification:

Article Title: Genome-wide analyses identify circAQR, a circular RNA suppressing proliferation but promoting migration and invasion in thyroid cancer.
Article Snippet: J circAQR expression accelerated themigration and invasion of BCPAP compared to the negative control group, as assessed by the Transwell assay. npj Precision Oncology | (2025) 9:171 9 Qiao Xin Zhou Biotechnology Co., Ltd. All cell lines were confirmed to be mycoplasma negative and were supplemented with 10% fetal bovine serum (Gibco, USA), 1% penicillin/streptomycin with RPMI 1640 medium at 37 °C,95% O2 and 5% CO2. .. We used EZ-press RNA Purification Kit (EZBioscience, USA) to extract total RNA from the cell lines according to the manufacturer’s instructions. cDNA was synthesized by using the Color Reverse Transcription Kit (EZBioscience, USA) according to the manufacturer’s instructions. .. Then, quantitative real-time PCR (qRT-PCR) was performed using SYBR Green qPCRMasterMixKit (EZBioscience,USA) according to themanufacturer’s instructions.

Synthesized:

Article Title: Genome-wide analyses identify circAQR, a circular RNA suppressing proliferation but promoting migration and invasion in thyroid cancer.
Article Snippet: J circAQR expression accelerated themigration and invasion of BCPAP compared to the negative control group, as assessed by the Transwell assay. npj Precision Oncology | (2025) 9:171 9 Qiao Xin Zhou Biotechnology Co., Ltd. All cell lines were confirmed to be mycoplasma negative and were supplemented with 10% fetal bovine serum (Gibco, USA), 1% penicillin/streptomycin with RPMI 1640 medium at 37 °C,95% O2 and 5% CO2. .. We used EZ-press RNA Purification Kit (EZBioscience, USA) to extract total RNA from the cell lines according to the manufacturer’s instructions. cDNA was synthesized by using the Color Reverse Transcription Kit (EZBioscience, USA) according to the manufacturer’s instructions. .. Then, quantitative real-time PCR (qRT-PCR) was performed using SYBR Green qPCRMasterMixKit (EZBioscience,USA) according to themanufacturer’s instructions.

Article Title: Loss of TACC2 impairs chemokine CCL3 and CCL4 expression and reduces response to anti-PD-1 therapy in soft tissue sarcoma.
Article Snippet: The antibodies used included anti-TACC2 (11407-1-AP, Proteintech), anti-Ki-67 (ab16667, Abcam), anti-CD4 (ab183685, Abcam), anti-CD8 (ab209775, Abcam), and anti-CD11b (ab133357, Abcam), along with goat anti-rabbit (PV6001, ZSGB-BIO) and goat anti-mouse (PV-6002, ZSGBBIO) secondary antibodies. .. Total RNA was extracted from tissues and cells using the TRIzolTM reagent (Sigma-Aldrich), and cDNAs were synthesized with the A0010CG Color Reverse Transcription Kit (EZBioscience, China) according to the manufacturer’s protocol. .. Quantitative real-time PCR (qRT-PCR) was performed using SYBR Green Master Mix (EZBioscience) on a Bio-Rad CFX96 PCR instrument.



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