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primescript fast rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript fast rt reagent kit
    Primescript Fast Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pmc13091134-83-11-20
    Average 99 stars, based on 89259 article reviews
    primescript fast rt reagent kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Single‐Cell Multiomics Integration Reveals Cell Trajectories and Regulatory Networks in Breast Cancer Progression
    Article Snippet: MCF‐7 (luminal, ER+/PR+/HER2‐, ATCC HTB‐22) and MDA‐MB‐231 (basal/TNBC, ATCC HTB‐26) cell lines were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 μ g/mL streptomycin at 37°C/5% CO2. .. Total RNA was extracted using TRIzol (Invitrogen); first‐strand cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara). .. Quantitative RT‐PCR was performed on a QuantStudio 5 system (Applied Biosystems) using SYBR Green Master Mix.

    Article Title: Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in Euglena gracilis
    Article Snippet: .. Complementary DNA was synthesized using PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Japan). .. qRT‐PCR was performed using TB Green Premix Ex Taq II Fast qPCR (Takara Bio, Japan) with a Thermal Cycler Dice Real Time System (Takara Bio, Japan).

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: Total RNA was extracted from serum samples and MC3T3-E1 cells subsequent to transfection and differentiation, utilizing TRIzol LS and TRIzol reagent (Invitrogen, Waltham, MA). .. cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP).

    Reverse Transcription:

    Article Title: AcNR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis.
    Article Snippet: .. Total RNA of A. cantonensis was extracted using Trizol (Invitrogen, USA) and reverse transcribed to cDNA using PrimeScript RT reagent Kit (Takara, Japan) after removing contaminating genomic DNA with DNase I (Sigma, USA). .. qPCR was performed using SYBR Green Real-Time PCR Master Mix (Toyobo, Japan) on a Light Cycler 480 Instrument II (Roche, Switzerland).

    Article Title: Pharmacological inhibition of REV-ERBα ameliorates motor deficits in the 6-OHDA-lesioned mouse model of Parkinson’s disease
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. .. For RT–PCR, 1 μg of each RNA sample was reverse-transcribed using the PrimeScript RT reagent (Takara Bio). .. Aliquots of the cDNA were then subjected to real-time qPCR in the presence of SYBR Green I (Enzynomics).

    Article Title: Ac NR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis
    Article Snippet: .. Total RNA of A. cantonensis was extracted using Trizol (Invitrogen, USA) and reverse transcribed to cDNA using PrimeScript RT reagent Kit (Takara, Japan) after removing contaminating genomic DNA with DNase I (Sigma, USA). .. qPCR was performed using SYBR Green Real-Time PCR Master Mix (Toyobo, Japan) on a Light Cycler 480 Instrument II (Roche, Switzerland).

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: Total RNA was extracted from serum samples and MC3T3-E1 cells subsequent to transfection and differentiation, utilizing TRIzol LS and TRIzol reagent (Invitrogen, Waltham, MA). .. cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP).

    cDNA Synthesis:

    Article Title: Genome-wide analysis of common structural variants in Alzheimer's disease identifies a risk-associated regulatory CCG repeat in FAM193B.
    Article Snippet: .. We performed cDNA synthesis with a PrimeScript RT Reagent Kit (Takara, RR037A) followed by quantitative polymerase chain reaction (qPCR) using Taq Pro Universal SYBR Master Mix (Vazyme, Q712). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Genome-wide analysis of common structural variants in Alzheimer's disease identifies a risk-associated regulatory CCG repeat in FAM193B.
    Article Snippet: .. We performed cDNA synthesis with a PrimeScript RT Reagent Kit (Takara, RR037A) followed by quantitative polymerase chain reaction (qPCR) using Taq Pro Universal SYBR Master Mix (Vazyme, Q712). ..



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