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primescript rt reagent reverse  (TaKaRa)


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    Structured Review

    TaKaRa primescript rt reagent reverse
    Primescript Rt Reagent Reverse, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+reverse-transcribed+reagent+kit/PrimeScript+RT+Reagent+Kit/pmc09749222-84-14-29
    Average 99 stars, based on 89544 article reviews
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    Immunoprecipitation:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Purification:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Ethanol Precipitation:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Reverse Transcription:

    Article Title: PRAME Augments K63‐Linked Ubiquitination and Stabilization of METTL3 to Promote Lung Adenocarcinoma Metastasis
    Article Snippet: .. The immunoprecipitated RNA was purified by ethanol precipitation and reverse‐transcribed into cDNA using a PrimeScript RT reagent kit (Takara). ..

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: .. Total RNA was extracted using the RNA Extraction Kit (Novizan, RC112- 01) and reverse- transcribed into cDNA with the PrimeScript RT Reagent Kit (TaKaRa, RR037A). .. qPCR was performed using iTaq Universal SYBR Green Supermix (Bio- Rad, 1 725 121) on a CFX Touch Real- Time PCR System (Bio- Rad).

    Article Title: Polyomavirus Drives Cerebellar Lesions in AIDS Monkeys With Ataxia
    Article Snippet: Genomic DNA was purified from tissues, SH‐SY5Y cells, and culture supernatants using a TIANamp Genomic DNA Kit (DP304, TIANGEN, China). .. RNA was reverse transcribed into cDNA using a PrimeScript FAST RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Nucleic acid concentrations were quantified with a NanoDrop ND2000 spectrophotometer (Thermo, USA).

    Article Title: ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns.
    Article Snippet: .. 2.6 Quantitative real-time PCR (qRT-PCR) Total RNA was isolated using TRIzol Invitrogen (#15596018, Invitrogen) according to the manufacturer’s instruction and was reverse transcribed by using PrimeScript RT reagent Kit (#RR037Q, Takara, Kusatsu, Shiga, Japan). .. Real time PCR was performed using TB Green Premix Ex Taq II (#RR820Q, Takara) on a Real time PCR system (#ABI7900HT, Applied Biosystems, Carlsbad, California).

    cDNA Synthesis:

    Article Title: Genome-wide identification of the TIFY gene family in Sorghum bicolor and functional characterization of SbTIFY9 in salt stress tolerance.
    Article Snippet: The TIFY gene family is a plant-specific group of transcriptional regulators characterized by a conserved TIFY domain (TIF[F/Y]XG), participating in plant development, phytohormone signaling and abiotic stress responses.. In this study, 21 SbTIFY genes were genome-widely identified in sorghum (Sorghum bicolor L.).. According to phylogeny and protein structures, SbTIFY proteins were grouped into JAZ and ZML subfamilies, and the JAZ subfamily was further divided into five clades.

    Real-time Polymerase Chain Reaction:

    Article Title: Biomimetic Dichalcogenide-Hybrid Nanozymes With Glutathione Peroxidase-Like Activity for Liver Protection.
    Article Snippet: Glutathione peroxidase (GPX)-mimetic nanozymes are a promising strategy to restore redox homeostasis, but many existing systems rely on metals and remain limited by cytotoxicity, poor selectivity, and susceptibility to catalytic deactivation.. Inspired by the active-site chemistry of native GPX, we designed dichalcogenide-hybrid mesoporous silica nanoparticles (MSNs) as inorganic–organic nanozymes with tunable sulfur and selenium environments.. Structure–activity analysis showed that GPXlike performance is governed by chalcogen composition, chalcogen density, and spatial proximity of S/Se within the framework, which together regulate reversible disulfide/diselenide redox cycling and substrate access.

    Article Title: Single-cell RNA sequencing identifies a proinflammatory S100a8/a9 + macrophage subset contributing to acute kidney injury.
    Article Snippet: 1 Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China 2 Kidney and Blood Purification Laboratory of Shanghai, Shanghai, China 3 Shanghai Medical Center of Kidney, Shanghai, China 4 Kidney and Dialysis Institute of Shanghai, Shanghai, China 5 Hemodialysis Quality Control Center of Shanghai, Shanghai, China Abstract Objective Macrophages represent a heterogeneous cell population in the kidney.. However, macrophage subsets and their role in endotoxemia-induced acute kidney injury (AKI) remain elucidated.. Material and methods Single-cell RNA sequencing was performed to identify macrophage subpopulations in mouse kidney following lipopolysaccharide (LPS) challenge.

    Article Title: ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns.
    Article Snippet: .. 2.6 Quantitative real-time PCR (qRT-PCR) Total RNA was isolated using TRIzol Invitrogen (#15596018, Invitrogen) according to the manufacturer’s instruction and was reverse transcribed by using PrimeScript RT reagent Kit (#RR037Q, Takara, Kusatsu, Shiga, Japan). .. Real time PCR was performed using TB Green Premix Ex Taq II (#RR820Q, Takara) on a Real time PCR system (#ABI7900HT, Applied Biosystems, Carlsbad, California).

    Polymerase Chain Reaction:

    Article Title: Single-cell RNA sequencing identifies a proinflammatory S100a8/a9 + macrophage subset contributing to acute kidney injury.
    Article Snippet: 1 Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China 2 Kidney and Blood Purification Laboratory of Shanghai, Shanghai, China 3 Shanghai Medical Center of Kidney, Shanghai, China 4 Kidney and Dialysis Institute of Shanghai, Shanghai, China 5 Hemodialysis Quality Control Center of Shanghai, Shanghai, China Abstract Objective Macrophages represent a heterogeneous cell population in the kidney.. However, macrophage subsets and their role in endotoxemia-induced acute kidney injury (AKI) remain elucidated.. Material and methods Single-cell RNA sequencing was performed to identify macrophage subpopulations in mouse kidney following lipopolysaccharide (LPS) challenge.

    RNA Extraction:

    Article Title: CDK4/6 Inhibitors and Curcumin Synergistically Suppress HER2- Breast Cancer via Lethal ROS and Inhibition of Metastasis.
    Article Snippet: .. Total RNA was extracted using the RNA Extraction Kit (Novizan, RC112- 01) and reverse- transcribed into cDNA with the PrimeScript RT Reagent Kit (TaKaRa, RR037A). .. qPCR was performed using iTaq Universal SYBR Green Supermix (Bio- Rad, 1 725 121) on a CFX Touch Real- Time PCR System (Bio- Rad).

    Isolation:

    Article Title: ZRANB1-mediated EZH2 stabilization drives S100B/S100A4 transcriptional activation and corneal neovascularization in alkali burns.
    Article Snippet: .. 2.6 Quantitative real-time PCR (qRT-PCR) Total RNA was isolated using TRIzol Invitrogen (#15596018, Invitrogen) according to the manufacturer’s instruction and was reverse transcribed by using PrimeScript RT reagent Kit (#RR037Q, Takara, Kusatsu, Shiga, Japan). .. Real time PCR was performed using TB Green Premix Ex Taq II (#RR820Q, Takara) on a Real time PCR system (#ABI7900HT, Applied Biosystems, Carlsbad, California).



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