qscript cdna supermix kit (Quanta Biosciences)

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Quanta Biosciences qscript cdna supermix kit
Qscript Cdna Supermix Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 4408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+reverse-transcribed+reagent+kit/qScript+cDNA+SuperMix/pmc05745906-109-10-17
Average 97 stars, based on 4408 article reviews

qscript cdna supermix kit - by Bioz Stars, 2026-10

97/100 stars

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RNA Extraction:

Article Title: Near-Infrared Light-Activated Polydopamine Nanoparticles Conjugated With Transferrin Peptides for Enhanced Brain Accumulation.
Article Snippet: The quantification of total RNA, as well as the purification levels, was performed using the NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). .. Following the RNA extraction, the cDNA synthesis was performed using qScript cDNA Supermix (Quantabio). .. The samples were amplified for 5 min at 25◦C, 30 min at 42◦C, 5 min at 85◦C, and held at 4◦C in the CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories, Inc).

cDNA Synthesis:

Article Title: Exercise volume modulates cardiac protection in a type 2 diabetic rat model: differential effects of high- and low-volume moderate-intensity endurance exercise training on diabetic cardiomyopathy
Article Snippet: Total RNA isolation was performed using an RNeasy Fibrous Tissue Kit (Qiagen Benelux B.V., Antwerp, Belgium), following the manufacturer’s protocol. .. RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Isogen Life Science B.V., Utrecht, Netherlands). cDNA synthesis was performed using a qScript cDNA SuperMix (QuantaBio, Leuven, Belgium). ..

Reverse Transcription:

Article Title: Dynamics of infectious salmon anemia virus in biofouling on well-boat hulls
Article Snippet: RNA was extracted using the Quick RNA Miniprep Kit (Zymo Research, USA). .. RNA samples (4 μL) were reverse transcribed using qScript cDNA SuperMix (QuantaBio, USA) according to manufacturer instructions to generate complementary DNA (cDNA). .. The resulting cDNA (4 μL) was used for RT-qPCR quantification of ISAV (QuantStudio 5, Thermo Fisher, USA) using ISAV-specific primers HPR-F: 5′-CGA AAG C-CCT GGA AAC TTT AGA-3′ and HPR-R: 5′-GAT GCC G-GAAGT-CGA TGA ACT-3′ (1 μL each, 500 nM final concentration) and probe HPR-HEX: HEX-5’AAG GCC AT-CGT CGC T-3’-NFQ (0,8 μL, 200 nM final concentration) (Christiansen et al. 2011) in 1X PerfeCTa MultiPlex qPCR ToughMix with ROX (QuantaBio, Massachusetts, USA) (4 μL), in a total reaction volume of 20 μL with nuclease-free water.

Concentration Assay:

Spectrophotometry:

other:

Article Title: Patterns of Proliferation in the Pacific Hagfish ( Eptatretus stoutii ) Retina Suggest an Ancient Origin for Adult Neurogenesis
Article Snippet: cDNA was made as per the manufacturer's instructions (Quantabio, Cat. No. 95048–100).

Synthesized:

Article Title: Autosomal recessive HOXA3 deficiency causes congenital athymia and laryngeal malformation.
Article Snippet: RNA was isolated from patient and control cells at the iPSC and TEP stages of differentiation using the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany). .. cDNA was synthesized using qScript cDNA SuperMix (Quantabio, Beverly, MA). .. Quantitative real-time PCR (qPCR) was performed on a 7500 Real-Time PCR System (Applied Biosystems, Waltham, MA) using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific).

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